LOCUS       sequence-467742-       14760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid with gRNA1 for site gsdA, Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14760)
  AUTHORS   Matthias Steiger
  TITLE     Regulation of the Glucose-6-phosphate dehydrogenase encoding gene 
            gsdA and its impact on growth and citric acid production in 
            Aspergillus niger
  JOURNAL   PLOS One
REFERENCE   2  (bases 1 to 14760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLOS One"
COMMENT     Sequence Label: pSF511
FEATURES             Location/Qualifiers
     source          1..14760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4696..5469)
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     ncRNA           complement(5485..5552)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     misc_RNA        complement(5557..5632)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           complement(5653..5689)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     rep_origin      7282..7870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7771..7790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11450..12475)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(11450..12475)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     regulatory      13059..13068
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      complement(13224..13997)
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     CDS             complement(14005..14025)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(14038..14760,1..3381))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(14038..14760,1..3381))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(14038..14760,1..3381))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gtcccaatct tttttgcgtg cgatcagctt gtcgctgttc cttttcggga ggatactttc
       61 cttggagaag cctccggtct gtacttcggt ctttttaacg atgttcacct gcggcatgga
      121 caggaccttc cggactgtcg cgaaatccct acccttgtcc cacacgattt ctcctgtttc
      181 tccgtttgtt tcgataagtg gtcgcttccg aatctctcca ttggccagtg taatctcggt
      241 cttgaaaaaa ttcataatat tgctgtaaaa gaagtactta gcggtggcct tgcctatttc
      301 ctgctcagac tttgcgatca ttttcctaac atcgtacact ttatagtctc cgtaaacaaa
      361 ttcagattca agcttgggat attttttgat aagtgcagtg cctaccactg cattcaggta
      421 ggcatcatgc gcatggtggt aattgttgat ctctctcacc ttataaaact gaaagtcctt
      481 tctgaaatct gagacgagct tagacttcag agtaataact ttcacctctc gaatcagttt
      541 gtcattttca tcgtacttgg tgttcatgcg tgaatcgaga atttgggcca cgtgcttggt
      601 gatctggcgt gtctcaacaa gctgcctttt gatgaagccg gctttatcca actcagacag
      661 gccacctcgt tcagccttag tcagattatc gaacttccgt tgtgtgatca gtttggcgtt
      721 cagcagctgc cgccaataat ttttcatttt cttgacaact tcttctgagg ggacgttatc
      781 actcttccct ctatttttat cggatcttgt caacacttta ttatcaatag aatcatcttt
      841 gagaaaagac tggggcacga tatgatccac gtcgtagtcg gagagccgat tgatgtccag
      901 ttcctgatcc acgtacatgt ccctgccgtt ctgcaggtag tacaggtaga gcttctcatt
      961 ctgaagctgg gtgttttcaa ctgggtgttc cttaaggatt tgggacccca gttcttttat
     1021 accctcttca atcctcttca tcctttccct actgttcttc tgtcccttct gggtagtttg
     1081 gttctctcgg gccatctcga taacgatatt ctcgggctta tgccttccca ttactttgac
     1141 gagttcatcc acgaccttaa cggtctgcag tattcccttt ttgatagctg ggctacctgc
     1201 aagattagcg atgtgctcgt gaaggctgtc cccctggcca gaaacttgtg ctttctggat
     1261 gtcctcctta aaggtgagag agtcatcatg gatcaactgc atgaagttcc ggttggcaaa
     1321 tccatcggac ttaagaaaat ccaggattgt ctttccactc tgcttgtctc ggatcccatt
     1381 gatcagtttt cttgacagcc gcccccatcc tgtatatcgg cgcctcttga gctgtttcat
     1441 gactttgtcg tcgaagagat gagcgtaagt tttcaagcgt tcttcaatca tctccctatc
     1501 ttcaaacaac gtaagggtga ggacaatgtc ctcaagaatg tcctcgttct cctcattgtc
     1561 caggaagtcc ttgtctttaa tgattttcag gagatcgtga tacgttccca gggatgcgtt
     1621 gaagcgatcc tccactccgc tgatttcaac agagtcgaaa cattcaatct ttttgaaata
     1681 gtcctctttg agctgtttca cggtaacttt ccggttcgtc ttgaagagga ggtccacgat
     1741 agctttcttc tgctctccag acaggaatgc tggctttctc atcccttctg tgacgtattt
     1801 gaccttggtg agctcgttat aaactgtgaa gtactcgtac agcagagagt gtttaggaag
     1861 caccttttcg ttaggcagat ttttatcaaa gttagtcatc ctttcgatga aggactgggc
     1921 agaggccccc ttatccacga cttcctcgaa gttccaggga gtgatagtct cttctgattt
     1981 gcgagtcatc cacgcgaatc tggaatttcc ccgggcgagg gggcctacat agtagggtat
     2041 ccgaaatgtg aggattttct caatcttttc cctgttatct ttcaaaaagg ggtagaaatc
     2101 ctcttgccgc ctgaggatag cgtgcagttc gcccaggtga atctggtggg ggatgcttcc
     2161 attgtcgaaa gtgcgctgtt tgcgcaacag atcttctctg ttaagcttta ccagcagctc
     2221 ctcggtgccg tccatttttt ccaagatggg cttaataaat ttgtaaaatt cctcctggct
     2281 tgctccgccg tcaatgtatc cggcgtagcc atttttagac tgatcgaaga aaatttcctt
     2341 gtacttctca ggcagttgct gtctgacaag ggccttcagc aaagtcaagt cttggtggtg
     2401 ctcatcatag cgcttgatca tactagcgct cagcggagct ttggtgatct ccgtgttcac
     2461 tcgcagaata tcactcagca gaatggcgtc tgacaggttc tttgccgcca aaaaaaggtc
     2521 tgcgtactgg tcgccgatct gggccagcag attgtcgaga tcatcatcgt aggtgtcttt
     2581 gctcagttga agcttggcat cttcggccag gtcgaagtta gatttaaagt tgggggtcag
     2641 cccgagtgac agggcgataa gattaccaaa caggccgttc ttcttctccc cagggagctg
     2701 tgcgatgagg ttttcgagcc gccgggattt ggacagccta gcgctcagga ttgctttggc
     2761 gtcaactccg gatgcgttga tcgggttctc ttcgaaaagc tgattgtaag tctgaaccag
     2821 ttggataaag agtttgtcga catcgctgtt gtctgggttc aggtccccct cgatgaggaa
     2881 gtgtccccga aatttgatca tatgcgccag cgcgagatag atcaaccgca agtcagcctt
     2941 atcagtactg tctacaagct tcttcctcag atgatatatg gttgggtact tttcatggta
     3001 cgccacctcg tccacgatat tgccaaagat tgggtggcgc tcgtgctttt tatcctcctc
     3061 caccaaaaag gactcctcca gcctatggaa gaaagagtca tccaccttag ccatctcatt
     3121 actaaagatc tcctgcaggt agcagatccg attctttctg cgggtatatc tgcgccgtgc
     3181 tgttcttttg agccgcgtgg cttcggccgt ctccccggag tcgaacagga gggcgccaat
     3241 gaggttcttc tttatgctgt ggcgatcggt attgcccaga actttgaatt ttttgctcgg
     3301 caccttgtac tcgtccgtaa tgacggccca accgacgctg tttgtgccga tatcgagccc
     3361 aatggagtac ttcttgtcca tgtgtcctgg tgggtgggtt gcaatgacgg cagacaaggc
     3421 aaggacgttt ggagatgagg gagtttggag gttgaggtga gtttgaagcc gcttcggccc
     3481 gtggtcgcct gcccttagtc agcgccgggg attgattggt tgcggagctt ctccggctgc
     3541 ccgcagtgac ctggtccgca gacctactcg attgggtgtc cggcttaggg cttagggtct
     3601 ataatctgct ccagtggtca tctgcggtct gccgtagata aggaacacat gccccggccg
     3661 agcaatggcc gcgttcagcc cgggatcagc cggtgctaaa ctgcgagagc ggacgctagt
     3721 gtcggttcat tgcgaacgaa caaatgccgg cgccatatgt tcccccgggc taaaatgaat
     3781 tatcatacat cacttaatac cttacatcaa cccgtcccta tagtgactca gagttatgct
     3841 gatcatcctc ccgtgcctac aacaagcctg caccaccagc cctatcggtg acccgacatt
     3901 tgaaccaata tggagagtaa ggagatcaga gggtccaagc agggccgaat ctggcgacag
     3961 tagatataaa tatgaaaatt ccccaacgct tttactcatg gagctgcatg gctttctcgc
     4021 ggcaagcagc tggggtgaag ccatttccaa ccttccctgc aatgatgcgc caccgatcgt
     4081 tctcgtactc gtgcattgcc cgctgcagcc tttgtaccta ttcccttcat tagcatgtgt
     4141 gagacaggcg tagaaataca tcaggagcaa atcgaaccat ttcgtcactc caagctacat
     4201 ccttagcctt cagcttggtg cagtatcgga cttgcaaggt accgctcgtt cggcccggga
     4261 agaagtctgc aatttgcttc cacgtcaagt tcttgttttc cttcaactcc accagtagag
     4321 cgtcctcttc gggagtaaac ttcagttttg gtcctcgagg cctgggggct ggatcaggca
     4381 ttccaggctg gcccaccaca ctcggcgggc ccgagggcaa aggaggagat tccacattca
     4441 ctccatgacg ccctggggcg cgcagcgctg catggggcgg gagtcgatgg tgatggtgat
     4501 gttgcggcag ggccacagag tccgtgggcc caaagccatg gtttgacatg ggatgctgct
     4561 gagatgatgc gccatggaca ccgaagagca tttcgggaga gtgcgagggt tcgtgagggg
     4621 cgtcagctga gaggactgat agctgcggcc gcaggccatg gtcggggttc ccatcctctt
     4681 cggttctccc cggagcgagt ggagatgtgg agtgggcgct tacacagtac acgaggactt
     4741 ctagaaagaa ggattacctc taaacaagtg tacctgtgca ttctgggtaa acgactcata
     4801 ggagagttgt aaaaaagttt cggccggcgt attgggtgtt acggagcatt cactaggcaa
     4861 ccatgcatgg ttactattgt atacccatct tagtaggaat gattttcgag gtttatacct
     4921 acgatgaatg tgtgtcctgt aggcttgaga gttcaaggaa gaaacagtgc aattatcttt
     4981 gcgaacccag gggctggtga cggaattttc atagtcaagc tatcagagta aagaagagga
     5041 gcatgtcaaa gtacaattag agacaaatat atagtcgcgt ggagccaaga gcggattcct
     5101 cagtctcgta ggactcttga cgaccgttga tctgcttgat ctcgtctccc gaaaatgaaa
     5161 atagactctg ctaagctatt cttctgcttc gccggagcct gaagggcgta ctagggttgc
     5221 gaggtccaat gcattaatgc attgcagatg agctgtatct ggaagaggta aacccgaaac
     5281 gcgttttatt cttgttgaca tggagctatt aaatcactag aaggcactct ttgctgcttg
     5341 gacaaatgaa cgtatcttat cgagatcctg aacaccattt gtctcaactc cggagctgac
     5401 atcgacacca acgatcttat atccagattc gtcaagctgt ttgatgattt cagtaacgtt
     5461 aagtggatca agcagtccaa agctgtccca ttcgccatgc cgaagcatgt tgcccagccg
     5521 gcgccagcga ggaggctggg accatgccgg ccaaaagcac cgactcggtg ccactttttc
     5581 aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa accccagcgt
     5641 tttcaccacc gtgacgagct tactcgtttc gtcctcacgg actcatcaga cggtgcatgt
     5701 ttgaagatgg atgagaagtc ggtgatgaga tagacgttct agcggtgatg gtgggttgat
     5761 taaggggtgt ggtgtgggtg gaggatgtga gggtggcggt gtgtgagtgt caaaatcgat
     5821 cgcgtgaggt gtgtcgtaag gagggatgtt caaggcccgc tgaatttata tcgacgccac
     5881 ggaagcttct cgagccctgg gatggcggag tcagaggggc ggtatgcgct agtccgcttt
     5941 gggttagggc tgctgacatc atggatcggc caatctttcc ccgtacgttg cccgattgtt
     6001 ggctgaggtt ctgggctgcg ctttgcttgg gggtttgtgt aaggtgagtc agagtgagtc
     6061 ggttgtctgg gattcttatt gattatgctg tgatgacagt ggtatttgtg cttagtgctg
     6121 attaggaata tgccatttgg gtggtgttgt ttggctgtaa ttattgtgct ggttcgtcca
     6181 tagggtgtat attactatat ctggaagtaa gttgagtctt gtatacggta gtataggtcc
     6241 gaggtgaaga gagataaaat caatacactg cttttaactg gtgtcatatt ttcacatcaa
     6301 tatgccctat tttattggcg ttcgctggct tttatagtgt gtaaagacat atacatgctc
     6361 agcgttattt gtccaaccca atatactgat gaactatgat tccgtggtcc agcacacacc
     6421 atgtcctcat cctgacgctg tttctactac gtcagtatac gtaaatataa tgtctggcag
     6481 aaggattctc ttgacttcca gacttaactt tcgaaaaaca atcaacgaaa atcaagggaa
     6541 agcagaggac gccatcaaag atgaccaccc acaagctctc tccgacatgc gagtcacatg
     6601 cgtcgctgat gctgaaggag actatccaca gaccctttga tctaatgtca gatatactca
     6661 ggcctatctg gctccgggtg atcaaagacg aacgctacaa tctcagacgt cgtctctatg
     6721 acagggcaag attatatgag aagcataggc caagcaagtg caacatccaa aaagactgga
     6781 tcaagcgcta caaggagaac gaggagctac agttcgccct cctgcagcgg attaactgtc
     6841 tggaaagggt caaatacaga cgtggtattg agccagaaaa tcccttccag catgtcccgg
     6901 ggcccgcgga tccgaaacgt caagtctccc ttgcagaaga gcattactac cggtgccaga
     6961 aggcatggaa agcgttgacc cttgacaaac catctggtcc tatcatcgac gagtttgact
     7021 tccttcagca tcacagaaac gaagagggcc aaactttggc atgggaggag gacaagctca
     7081 tgtgcgcctc gcttggtggc tgttgcggtc gcagatgtag atgctgtgag agtcctctgc
     7141 ggcactatct cttgcccgag ggcggccaga aggaaatgat tggatttcat ggccactgta
     7201 cctccgatcg cgaaaaaacc ccgccctgtc aggggcgggg ttttttgcgc tccaccccgt
     7261 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7321 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7381 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7441 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7501 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7561 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7621 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7681 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7741 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7801 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7861 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7921 tgctcacagc gccactggac tctcaggctt tccattgggg tactaacata gccatcaaat
     7981 gccggcccgt gtttaatctc ctcgagatgg ctgggctcac atgcgttcta ctcaaaaact
     8041 cttataccta ggccctctcc caatcgtgta tgtttggacc ttcctctctc acactttgga
     8101 taacatcaag catactgcag attttgacct agttaacaag gcacatcatg ctatcacttc
     8161 caacactctt gctgagcgcg ttctgccttg cctttctggt tctacgaagt atccagtccc
     8221 gcctgcaacg ccgcagaaac gccaaaaggt ggggttgtca accgccagct agagctccat
     8281 cgggattctt tggcattccg ccgttcctcg gtggtcgcag ataactccct aaattccctg
     8341 agctaactct aagtcgacca tgccgtttat ggttagcgcc tcccaaaaag gaatggccga
     8401 cttagagtta cctcttgacc gactttttct ttcctccccc ttacatttcg ttaccacaac
     8461 acattcctat atccaaactc caggtacata actagtcgaa atctctttta aatctagtca
     8521 agaactagat acttaacctt catactgctt agagatagcc tttgaagctc ttatttcgct
     8581 ctttgtctca ctctcacctt cctcctcctc ctccaatcct ttttgctcgg gactagtcca
     8641 agactagtca acgattagta tgccgagcat tcgcgataac gatctccgaa agtccccaga
     8701 gtactgtcac tatctcgagg cagttaagga cggggagctt acgctgccgg atttcaagat
     8761 agtaagccga cccgactagc tttaagccta gttactgact agtccacagg acgacaatgg
     8821 cgtgcctgat atccatccat atgaagtcta ctgccgagtg aagggatgcc tcaagcgtac
     8881 agtgagtcta ctgctatact agtttctgac tagttattaa ctagttgaca ggttccctct
     8941 gccaacagaa acatattggt caagcacttg aaggacaaga actcccacgg catggagttt
     9001 acattgcaca atggacctcc cactatgaag gaactgatgg aggccaaagg caagtcctat
     9061 ctagattact tggtgactag tccctgacta gtcaagtagc atggtatgaa ggcttgtttg
     9121 aaggcactgt tctcccaacc ccgactccta ccaagaagcg caagcgagct gcgtaagttt
     9181 ctgtgagtct aactagtgta ttagctaata tatagcagca ccaagtccaa ggaccacaat
     9241 actaagggtg tcgagaattc gtgagtttct tctcccattt caactagtcc ttgactagtc
     9301 actaactact ttgcagcaac gagggtgaag ctggaaatga tcaggacaat ggcgagggcc
     9361 cgtaagtaca gccattcaat gcagactagt tgctaactaa tctgtgacta gttcaagtgg
     9421 tccgtacgcc gtgcataccc ctgtgactgg taggaatttg agcaagcctg tcttgccgcg
     9481 cgatgagaaa ggaaaggcaa gttacattca gccccgtacc taggatcagt gctaatttat
     9541 aacctctagc cactctttat gcagatccgc cgtgagggta gcaaggcagc taaatcagct
     9601 ggtgagaaag gaaccatacc ctgcaagacc tgtcgcaacg caaagggcaa aggtaagcta
     9661 tccaagctag tttgggacta gattctaact agtctcagca ccgtgtggtt caaagccata
     9721 ttgcgagttt tggcgctttt tctcatcgat tgacgaggca aagggagcga gtatgcaacc
     9781 tcgtaagtca gactcagaca aagaccaact agttattgac cagtcatcta gaaggctctg
     9841 ttgtggatct tgaggccctg gagagttcct ccaacaatcc ggagacaagc aagtcctcgt
     9901 cggactagtc actaactaga ctctaactag ttgcagacat ggataatgca aaagagacaa
     9961 gcaatgaaga aagtggtaag acatttctcc tttgtggttc tggactagtc tttgactagt
    10021 cacagtctta aacaaggaaa atgagcatga aaatgaggag gaaaaggctg ctgagcccga
    10081 ggaagtgcag ggtgatggca gacatggtag gttaatacct tgttagttat tgctagtcac
    10141 tgactagtca ataactagtc tctgaacacc ttgcaatcac tccgtttgcg cagctgaaca
    10201 gtggtgagga taatagtagt aagttattct agcttcagag ttataggaga ctagatacta
    10261 actagtatta gttgcaacta acctggatct cagagacttt ggcctcaatc tagaatctat
    10321 ctagttgtca actagactgt ggtatcattg tcttttattt tcctagtcct ggaactagct
    10381 tctaactagt ctccctaata tgtggctgtc ttgttttttt tttttgtttc cctacccgga
    10441 tatctagtcc ccttctaggt tctgttaacc tctcgggctc tgatttagtt taacgcaaac
    10501 ctgagattag tttctaacta gtctctaggt tttctatcca cctttaattg taataataaa
    10561 tacaagcaac gtttatacgt caaaagcatt tataaacttt taccctaaag tagcttgctt
    10621 gtgtgtttag tttataatta gtctcttatt aatttgatgt aggtaagccc gccacaaata
    10681 tatattttta acaagatacc gtggaaaaac ttcgtgctat cacaaaacag tatacaaaaa
    10741 ataagcttgt cgacgaattc agatatcacg aattctggat ccgatacgta acgcgtctgc
    10801 agcatctccg cgtaacgcgt ctgcagcatc tccgccgtaa cgcgtctgca gcataagcta
    10861 ttctgcatta acgtcagagc acaggctggt taacatgacc agactgatcg ccataggctc
    10921 atgctccgca gctgtaggca atttgggtat ggtttccacg tgcacttaca tgtcatggag
    10981 aagcgcaaga ggtggagtaa ttggaatcta ccagcttgat ctataaacag aaattttggt
    11041 tgcgatggat cagtacagcc atgttgcacc ccgtgtatcc tcattcaagc aaggttgcgc
    11101 agttggcaaa gccctgctcc tgtcacgacg tgctttcgct ggtactcgga gttgttatac
    11161 gctactcgac tgccgaatcg atctttagat cacagacaca gttgacttta ttctcggagt
    11221 atgtctaata gacactcgac attgtggata gatgttctct ctctctcact agctactgat
    11281 cgacaaagac catccatgtg aattatagta tcgctggcaa caatccatca gttgctcatt
    11341 tgcggtctac cgacctgcga cagacaacca aagtagacaa agactccgcc aaccgggaac
    11401 cacaccatcg cacatcattg tttgaagccc ggtcacgaaa gccatgcatc tattcctttg
    11461 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
    11521 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
    11581 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
    11641 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
    11701 atcctgcaag ctccgggtgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
    11761 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
    11821 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
    11881 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
    11941 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
    12001 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
    12061 gcggtccgaa tgggccgaac ccgctcgtct ggctgagatc ggccgcagcg atcgcatcca
    12121 tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
    12181 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt
    12241 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
    12301 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
    12361 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
    12421 acttttcgat caggaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatctaga
    12481 ggttaagagg ttcttccggc ttcgcgataa ggtactggag gagaaacaaa agtcaagccg
    12541 gtcgggaggg ggggagagaa atcagcaggt gaggctcacg agtcaggtca tcgtcaagct
    12601 cccgtgagtg aaaaaagaga cgacgataag gcgatgcgtg caaaagagat gcagacaaag
    12661 gggcagatgg accaaaggca gacaaggatg gagagggaaa taaataaggg aacaagggcg
    12721 gcttcgacga gggagagaga aaaaaaaaac aacctgagct gcgggaaggc gagccaagat
    12781 taggtgatgc tgcgcgctga gtgtgcaggt ggtctggggt ggagggaggg gggtggcaga
    12841 gccggcagcc gtcagtggtt ggattttttt tttttttttt ctcacttcaa cctcaccttt
    12901 tttcccctct ctctctctct ctctctctcc tctctctata ctgtacctag caataatagc
    12961 tcatggactc acagcaacgc tctgtgagcc aaccaagggc ccggattggc cacagcagcc
    13021 acgacaaagc acggcatccc tccgttggtt cgctgctccc atggcggccg ctttgctcca
    13081 actcaggcga tgctgcccct ccatccttgc cctcgcctcg ccagtgaggg gcattgagtt
    13141 gagcgctaac tgaggggcag gccaacgtgg aacagctggg ataaggggac cggccgctag
    13201 tctcaccgtt attcatgaat tagcgagtgg agatgtggag tgggcgctta cacagtacac
    13261 gaggacttct agaaagaagg attacctcta aacaagtgta cctgtgcatt ctgggtaaac
    13321 gactcatagg agagttgtaa aaaagtttcg gccggcgtat tgggtgttac ggagcattca
    13381 ctaggcaacc atgcatggtt actattgtat acccatctta gtaggaatga ttttcgaggt
    13441 ttatacctac gatgaatgtg tgtcctgtag gcttgagagt tcaaggaaga aacagtgcaa
    13501 ttatctttgc gaacccaggg gctggtgacg gaattttcat agtcaagcta tcagagtaaa
    13561 gaagaggagc atgtcaaagt acaattagag acaaatatat agtcgcgtgg agccaagagc
    13621 ggattcctca gtctcgtagg actcttgacg accgttgatc tgcttgatct cgtctcccga
    13681 aaatgaaaat agactctgct aagctattct tctgcttcgc cggagcctga agggcgtact
    13741 agggttgcga ggtccaatgc attaatgcat tgcagatgag ctgtatctgg aagaggtaaa
    13801 cccgaaacgc gttttattct tgttgacatg gagctattaa atcactagaa ggcactcttt
    13861 gctgcttgga caaatgaacg tatcttatcg agatcctgaa caccatttgt ctcaactccg
    13921 gagctgacat cgacaccaac gatcttatat ccagattcgt caagctgttt gatgatttca
    13981 gtaacgttaa gtggatcaag ctcacacctt cctcttcttc ttggggtcag ccctgctgtc
    14041 tccaccgagc tgagagaggt cgattcttgt ttcatagagc cccgtaattg actgatgaat
    14101 cagtgtggcg tccaggacct cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc
    14161 gaagtacttg aaggctgcag gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt
    14221 ttctgcctgc tccctgatgg gcttatccct gtgcttattg taagcagaaa gcaccttatc
    14281 gaggttagcg tcggcgagga tcactctttt ggagaattcg cttatttgct cgatgatctc
    14341 atcaaggtag tgtttgtgtt gttccacgaa cagctgcttc tgctcattat cttcggggga
    14401 ccctttgagc ttttcatagt ggctggccag atacaagaaa ttaacgtatt tagagggcag
    14461 tgccagctcg ttacctttct gcagctcgcc cgcactagcg agcattcgtt tccggccgtt
    14521 ttcaagctca aagagagagt acttgggaag cttaatgatg aggtcttttt tgacctcttt
    14581 atatcctttc gcctcgagaa agtcgatggg gtttttttcg aagcttgatc gctccatgat
    14641 tgtgatgccc agcagttcct tgacgctttt gagtttttta gacttccctt tctccacttt
    14701 ggccacaacc agtacactgt aagcgactgt aggagaatcg aatccgccgt atttcttggg
//
