LOCUS       sequence-46777-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RFXANK (3V3O)
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             631..648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(701..719)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      715..763
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatattttgt ttaactttaa gaaggagata taccatgcat catcatcatc atcacagcag
       61 cggcagagaa aacttgtatt tccagggcga ctccctgtcc atccaccagc tcgcagcaca
      121 gggggagctg gaccagctga aggagcattt gcggaaaggt gacaacctcg tcaacaagcc
      181 agacgagcgc ggcttcaccc ccctcatctg ggcctccgcc tttggagaga ttgagaccgt
      241 tcgcttcctg ctggagtggg gtgccgaccc ccacatcctg gcaaaagagc gagagagcgc
      301 cctgtcgctg gccagcacag gcggctacac agacattgtg gggctgctgc tggagcgtga
      361 cgtggacatc aacatctatg attggaatgg agggacgcca ctgctgtacg ctgtgcgcgg
      421 gaaccacgtg aaatgcgttg aggccttgct ggcccgaggc gctgacctca ccaccgaagc
      481 cgactctggc tacaccccga tggaccttgc cgtggccctg ggataccgga aagtgcaaca
      541 ggtgatcgag aaccacatcc tcaagctctt ccagagcaac ctggtgcccg ctgaccctga
      601 gtgatgacga agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      661 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      721 taaccccttg ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      781 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      841 gttacgcgca gcgtgaccgc tacacttgcc agcg
//
