LOCUS       sequence-467813-        9190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional reporter for detecting calcium increase in HEK293T 
            (transcription factor) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Zhou G, Li R, Bartolik O, Ma Y, Wan WW, Meng J, Hu Y, Ye B, Wang W
  TITLE     An improved FLARE system for recording and manipulating neuronal 
            activity.
  JOURNAL   Cell Rep Methods. 2025 Apr 21;5(4):101012. doi: 
            10.1016/j.crmeth.2025.101012. Epub 2025 Mar 21.
  PUBMED    40120579
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2025-04-21"; volume: "5(4):101012. doi"; pages: " 
            10.1016/j.crmeth.2025.101012. Epub 2025 Mar 21"
COMMENT     Sequence Label: pLX208-cytoFLARE1.0-TF
FEATURES             Location/Qualifiers
     source          1..9190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1847..1964
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2081..2384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2385..2588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2539..2559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2623..3558
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4171..4191
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             4204..4224
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4225..4662
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4663..5004
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             5005..5028
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5047..5635
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5100..5120)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5518..5529)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5707..5940
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6018..6139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6055..6074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6109..6128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6179..6314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6334..6353)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6335..6353)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6363..6380)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6363..6379)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6372..6394)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6521..6976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6608..6627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6818..6839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7002..7106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7107..7175,7176..7967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7325..7344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8138..8726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8627..8646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8880..8897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9014..9035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9050..9055,9056..9073,9074..9080)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9088..9104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9093..9115
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9112..9128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9112..9128
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9147..9167
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9149..9167
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagagcg ctgccgtctg gaccgatggc tgtgtggaag
     1741 tgctcgccga tagtggaaac agacgcccca gcactcgtcc tagggcaaag gatctgcagt
     1801 aatgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     1861 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1921 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1981 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     2041 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2101 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2161 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2221 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2281 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2341 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2401 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2461 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2521 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2581 gcagagctct ctggctaact gtcgggatcc accatgccat ctgctggaga catgagagct
     2641 gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag cctggccttg
     2701 tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat
     2761 tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac
     2821 ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg
     2881 gatttgaccc tccatgatca ggtccacctt ctagaatgtg cctggctaga gatcctgatg
     2941 attggtctcg tctggcgctc catggagcac ccagtgaagc tactgtttgc tcctaacttg
     3001 ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg
     3061 ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc
     3121 aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct
     3181 ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac
     3241 ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc
     3301 ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg
     3361 aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg aggcggcgga cgcccaccgc
     3421 ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg
     3481 gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca
     3541 gagggtttcc ctgccacagc tgctagcttc aacgctcgca ggaagctgaa gggtgctatc
     3601 ctgttcacca tgctcgccac aagaaacttc agcggcagct ttaatgcgcg gcgcaagcta
     3661 aagggagcca tacttttcac aatgttggct accagaaatt tttcggagct ggccgagaag
     3721 ctggccggcc tggacatcaa cggtggagct agcgagtttc gggcaaccac actggaacgg
     3781 atcgagaaaa gcttcgtgat tactgatccg agactgcctg acaacccagt gatttttgtg
     3841 agcgattcct tcctgcagct gacagaatat tctcgggaag agatcctggg gcgcaattgc
     3901 cgttttctgc agggacccga gacagaccgt gccactgttc ggaaaatcag agatgctatt
     3961 gacaaccaga ctgaagtgac cgttcagctg atcaattata ccaagagcgg caagaagttc
     4021 tggaacgtgt tccacctgca gccgatgcgc gattataagg gcgacgtcca gtacttcatt
     4081 ggcgtgcagc tggatggcac cgaacgtctt catggcgccg ctgagcgtga ggcggtctgc
     4141 ctggtcaaaa agacagcctt tcagattgct gagaacctgt acttccagat gggcggaacc
     4201 ggtcccaaga agaagagaaa ggtgaagcta ctgtcttcta tcgaacaagc atgcgatatt
     4261 tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag
     4321 aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat
     4381 ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttccg
     4441 cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta
     4501 acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca
     4561 gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg
     4621 gaagagagta gtaacaaagg tcaaagacag ttgactgtat cggccaattt taatcaaagt
     4681 gggaatattg ctgatagctc attgtccttc actttcacta acagtagcaa cggtccgaac
     4741 ctcataacaa ctcaaacaaa ttctcaagcg ctttcacaac ctattgcctc ctctaacgtt
     4801 catgataact tcatgaataa tgaaatcacg gctagtaaaa ttgatgatgg taataattca
     4861 aaaccactgt cacctggttg gacggaccaa actgcgtata atgcctttgg aatcactaca
     4921 gggatgttta ataccactac aatggatgat gtatataact atctattcga tgatgaagat
     4981 accccaccaa acccaaaaaa agaggactac aaggatgacg acgataagta gacgcgttaa
     5041 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5101 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5161 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5221 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5281 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5341 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5401 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5461 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5521 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5581 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     5641 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5701 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5761 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5821 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5881 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5941 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6001 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6061 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6121 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6181 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6241 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6301 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     6361 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6421 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6481 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6541 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6601 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6661 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6721 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6781 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6841 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6901 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6961 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7021 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7081 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7141 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7201 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7261 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7321 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7381 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7441 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7501 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7561 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7621 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7681 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7741 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7801 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7861 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7921 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7981 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8041 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8101 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8161 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8221 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8281 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8341 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8401 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8461 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8521 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8581 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8641 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8701 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8761 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8821 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8881 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8941 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9001 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9061 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9121 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     9181 agctgcaagc
//
