LOCUS       sequence-467814-        9649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional reporter for detecting calcium increase in HEK293T 
            (transcription factor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Zhou G, Li R, Bartolik O, Ma Y, Wan WW, Meng J, Hu Y, Ye B, Wang W
  TITLE     An improved FLARE system for recording and manipulating neuronal 
            activity.
  JOURNAL   Cell Rep Methods. 2025 Apr 21;5(4):101012. doi: 
            10.1016/j.crmeth.2025.101012. Epub 2025 Mar 21.
  PUBMED    40120579
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2025-04-21"; volume: "5(4):101012. doi"; pages: " 
            10.1016/j.crmeth.2025.101012. Epub 2025 Mar 21"
COMMENT     Sequence Label: pLX208-FLiCRE2-TF
FEATURES             Location/Qualifiers
     source          1..9649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1847..1964
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2081..2384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2385..2588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2539..2559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     sig_peptide     2614..2673
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             2698..2724
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2725..2751
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3499..4008
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             4630..4650
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             4663..4683
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4684..5121
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5122..5463
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             5464..5487
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5506..6094
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5559..5579)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5977..5988)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6166..6399
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6477..6598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6514..6533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6568..6587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6638..6773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6793..6812)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6794..6812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6822..6839)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6822..6838)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6831..6853)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6980..7435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7067..7086)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7277..7298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7461..7565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7566..7634,7635..8426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7784..7803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8597..9185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9086..9105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9339..9356
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9473..9494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9509..9514,9515..9532,9533..9539)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9547..9563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9552..9574
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9571..9587
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9571..9587
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9606..9626
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9608..9626
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagagcg ctgccgtctg gaccgatggc tgtgtggaag
     1741 tgctcgccga tagtggaaac agacgcccca gcactcgtcc tagggcaaag gatctgcagt
     1801 aatgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     1861 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1921 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1981 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     2041 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2101 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2161 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2221 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2281 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2341 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2401 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2461 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2521 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2581 gcagagctct ctggctaact gtcgggatcc accatggaga cagacacact cctgctatgg
     2641 gtactgctgc tctgggttcc aggttccact ggtgacgggg cccagccggc cagatcttac
     2701 ccatacgatg tgccagatta cgcctatccc tatgacgtcc ctgattacgc tctcgacttc
     2761 cagaaggcct ccagcatagt ctataagaaa gagggggaac aggtggagtt ctccttccca
     2821 ctcgccttta cagttgaaaa gctgacgggc agtggcgagc tgtggtggca ggcggagagg
     2881 gcttcctcct ccaagtcttg gatcaccttt gacctgaaga acaaggaagt gtctgtaaaa
     2941 cgggttaccc aggaccctaa gctccagatg ggcaagaagc tcccgctcca cctcaccctg
     3001 ccccaggcct tgcctcagta tgctggctct ggaaacctca ccctggccct tgaagcgaaa
     3061 acaggaaagt tgcatcagga agtgaacctg gtggtgatga gagccactca gctccagaaa
     3121 aatttgacct gtgaggtgtg gggacccacc tcccctaagc tgatgctgag cttgaaactg
     3181 gagaacaagg aggcaaaggt ctcgaagcgg gagaaggcgg tgtgggtgct gaaccctgag
     3241 gcggggatgt ggcagtgtct gctgagtgac tcgggacagg tcctgctgga atccaacatc
     3301 aaggttctgc ccacatggtc caccccggtg cagccaatgg ccctgattgt gctggggggc
     3361 gtcgccggcc tcctgctttt cattgggcta ggcatcttct tctgtgtcag gtgccggcac
     3421 cgaaggcgca agggctcggg ctcgacctcg ggctcgggca gcggtggctc tagaggttct
     3481 ggtggcagct ctggaggtat gaatggagct ataggaggtg accttttgct caattttcct
     3541 gacatgtcgg tcctagagcg ccaaagggct cacctcaagt acctcaatcc cacctttgat
     3601 tctcctctcg ccggcttctt tgccgattct tcaatgatta ccggcggcga gatggacagc
     3661 tatctttcga ctgccggttt gaatcttccg atgatgtacg gtgagacgac ggtggaaggt
     3721 gattcaagac tctcaatttc gccggaaacg acgcttggga ctggaaattt caaggcagcg
     3781 aagtttgata cagagactaa ggattgtaat gaggcggcga agaagatgac gatgaacaga
     3841 gatgacctag tagaagaagg agaagaagag aagtcgaaaa taacagagca aaacaatggg
     3901 agcacaaaaa gcatcaagaa gatgaaacac aaagccaaga aagaagagaa caatttctct
     3961 aatgattcat ctaaagtgac gaaggaattg gagaaaacgg attatattca tggtggctct
     4021 ggtagcttca acgctcgcag gaagctgaag ggtgctatcc tgttcaccat gctcgccaca
     4081 agaaacttca gcggcagctt taatgcgcgg cgcaagctaa agggagccat acttttcaca
     4141 atgttggcta ccagaaattt ttcggagctg gccgagaagc tggccggcct ggacatcaac
     4201 ggtggagcta gcgagtttcg ggcaaccaca ctggaacgga tcgagaaaag cttcgtgatt
     4261 actgatccga gactgcctga caacccagtg atttttgtga gcgattcctt cctgcagctg
     4321 acagaatatt ctcgggaaga gatcctgggg cgcaattgcc gttttctgca gggacccgag
     4381 acagaccgtg ccactgttcg gaaaatcaga gatgctattg acaaccagac tgaagtgacc
     4441 gttcagctga tcaattatac caagagcggc aagaagttct ggaacgtgtt ccacctgcag
     4501 ccgatgcgcg attataaggg cgacgtccag tacttcattg gcgtgcagct ggatggcacc
     4561 gaacgtcttc atggcgccgc tgagcgtgag gcggtctgcc tggtcaaaaa gacagccttt
     4621 cagattgctg agaacctgta cttccagatg ggcggaaccg gtcccaagaa gaagagaaag
     4681 gtgaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
     4741 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac
     4801 tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg
     4861 ctagaaagac tggaacagct atttctactg atttttccgc gagaagacct tgacatgatt
     4921 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
     4981 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
     5041 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
     5101 caaagacagt tgactgtatc ggccaatttt aatcaaagtg ggaatattgc tgatagctca
     5161 ttgtccttca ctttcactaa cagtagcaac ggtccgaacc tcataacaac tcaaacaaat
     5221 tctcaagcgc tttcacaacc tattgcctcc tctaacgttc atgataactt catgaataat
     5281 gaaatcacgg ctagtaaaat tgatgatggt aataattcaa aaccactgtc acctggttgg
     5341 acggaccaaa ctgcgtataa tgcctttgga atcactacag ggatgtttaa taccactaca
     5401 atggatgatg tatataacta tctattcgat gatgaagata ccccaccaaa cccaaaaaaa
     5461 gaggactaca aggatgacga cgataagtag acgcgttaag tcgacaatca acctctggat
     5521 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5581 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5641 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5701 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5761 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5821 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5881 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     5941 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6001 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6061 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     6121 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6181 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6241 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6301 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6361 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     6421 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6481 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6541 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6601 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6661 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6721 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     6781 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6841 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6901 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6961 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7021 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7081 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7141 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7201 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7261 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7321 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7381 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     7441 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7501 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7561 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7621 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7681 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7741 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7801 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7861 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7921 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7981 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8041 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8101 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8161 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8221 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8281 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8341 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8401 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8461 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8521 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8581 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8641 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8701 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8761 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8821 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8881 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8941 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9001 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9061 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9121 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9181 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9241 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9301 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9361 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9421 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     9481 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9541 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     9601 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
