LOCUS       sequence-46782-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: USP37
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             450..467
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(520..538)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      534..581
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(705..724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     918..939
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt ctgaagatac atggtcctat cagaattcga
      121 agtatgcaga ctgggattac aaagtggaaa gaaggatcct ttgaaattgt agaaaaagag
      181 aataaagtca gcctagtagt tcactacaat actggaggaa ttccaaggat atttcagcta
      241 agtcataaca ttaaaaatgt ggtgcttcga cccagtggag cgaaacaaag ccgcctaatg
      301 ttaactctgc aagataacag cttcttgtct attgacaaag taccaagtaa ggatgcagag
      361 gaaatgaggt tgtttctaga tgcagtccat caaaacagac ttcctgcagc catgaaaccg
      421 tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg
      481 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      541 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      601 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggggctcc
      781 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      841 atggttcacg tagtgggcca tcgccctgat agacggtttt ttcgcccttt tgacgttgga
      901 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaaca
//
