LOCUS       sequence-46783-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LMNB2
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             495..512
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(565..583)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      579..626
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(750..769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgggtaattc ctctgaatat tttgtttact ttaagaagga gatataccat gcatcatcat
       61 catcatcaca gcagcggcag agaaaacttg tatttccagg gctcggcctc gggtagcgtc
      121 agcatcgagg agatcgacct ggagggcaag tttgtgcagc tcaagaacaa ctcggacaag
      181 gatcagtctc tggggaactg gagaatcaag aggcaggtct tggaggggga ggagatcgcc
      241 tacaagttca cgcccaagta catcctgcgc gccggccaga tggtcacggt gtgggcagct
      301 ggtgcggggg tggcccacag ccccccctcg acgctggtgt ggaagggcca gagcagctgg
      361 ggcacgggcg agagcttccg caccgtcctg gttaacgcgg atggcgagga agtggccatg
      421 aggactgtga agaagtcctc ggtgatgcgt gagaatgaga atgggtgatg acgaagcttg
      481 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      541 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      601 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      661 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      721 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      781 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg gctcccttta gggttccgat
      841 ttagtgcttt acggcaccct cgaccccaaa aacttgatta ggtgatg
//
