LOCUS       sequence-46784-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: DMTF1
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             282..299
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(352..370)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      366..413
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      450..905
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(537..556)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     747..768
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcgac agaaaccatg tgggaaaata tacacctgaa
      121 gaaattgaga agctcaagga gctccggata aagcatggca atgactgggc aacaataggg
      181 gcggcgctag gaagaagtgc atcttctgtc aaagatcggt gccgactgat gaaggatact
      241 tgcaacacag ggtgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact
      301 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      361 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      421 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      481 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      541 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      601 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      661 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      721 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      781 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
      841 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
      901 aattta
//
