LOCUS       sequence-46786-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RTF1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             535..552
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(605..623)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      619..666
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(790..809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggccct cccaaatccc aaccagtttc
      121 cttacctgaa gaattgaatc gggttcgatt atcacggcat aagctagaac gctggtgtca
      181 catgcccttc tttgctaaaa ctgtcacagg atgttttgtg cggattggca tcggaaacca
      241 caacagcaaa ccagtttacc gggtcgctga gattacgggt gttgtggaaa ctgccaaagt
      301 ttaccaacta ggtggcacca gaacaaacaa agggctgcaa ctacggcatg gcaatgacca
      361 acgcgtgttc cgtttagagt ttgtctcaaa ccaagaattc accgaaagtg agtttatgaa
      421 gtggaaagaa gcgatgttct ctgctggcat gcagttgccc actctagatg aaatcaataa
      481 aaaggaatta tctattaaag aagcttgatg acgaagcttg cggccgcact cgagcaccac
      541 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      601 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      661 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      721 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      781 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      841 gtcaagctct aaatcggggg ctcccctttt agggttccga nttagtgctt tacggcaccc
      901 tcgaccccaa aaaactgata ggtgatgtcc
//
