LOCUS       sequence-467895-        8721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a EnAsCas12a guide targeting FOCAD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Borck PC, Boyle I, Jankovic K, Bick N, Foster K, Lau AC, 
            Parker-Burns LI, Lubicki DA, Li T, Borah AA, Lofaso NJ, Das Sharma 
            S, Chan T, Kishen RV, Adeagbo A, Raghavan S, Aquilanti E, Prensner 
            JR, Krill-Burger JM, Golub TR, Campbell CD, Dempster JM, Chan EM, 
            Vazquez F
  TITLE     SKI complex loss renders 9p21.3-deleted or MSI-H cancers dependent 
            on PELO.
  JOURNAL   Nature. 2025 Feb 5. doi: 10.1038/s41586-024-08509-3.
  PUBMED    39910293
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Feb 5. doi: 10.1038/s41586-024-08509-3."
COMMENT     Sequence Label: pRDA052H_sgFOCAD #2
FEATURES             Location/Qualifiers
     source          1..8721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2776..3024
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2776..2796
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2947..2966
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    3137..3254
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3393..4571
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3624..4562
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4519..4539
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4584..5603
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    5631..6219
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5684..5704)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6102..6113)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6291..6524
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6602..6723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6639..6658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6693..6712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6763..6898
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6918..6937)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6919..6937)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6947..6964)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6947..6963)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6956..6978)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7105..7560
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7192..7211)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7402..7423
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7586..7690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7691..7759,7760..8551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7909..7928)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccagag gtaccgaggg cctatttccc atgattcctt catatttgca tatacgatac
     2821 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
     2881 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     2941 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     3001 atcttgtgga aaggacgaaa caccggtaat ttctactctt gtagataatc accccaacta
     3061 acctccaggt tttttgaatt cgctagcaag cttggcgtaa ctagatcttg agacaaatgg
     3121 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     3181 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     3241 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
     3301 agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
     3361 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
     3421 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct
     3481 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     3541 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     3601 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     3661 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
     3721 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     3781 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     3841 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3901 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3961 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     4021 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     4081 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     4141 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     4201 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     4261 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     4321 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     4381 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     4441 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     4501 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     4561 aggtgtcgtg acgtacggcc accatgaaaa agcctgaact caccgctacc tctgtcgaga
     4621 agtttctgat cgaaaagttc gacagcgtat ccgacctgat gcagctctcc gagggcgaag
     4681 aatctcgggc tttcagcttc gatgtgggag ggcgtggata tgtcctgcgg gtgaatagct
     4741 gcgccgatgg tttctacaaa gatcgctatg tttatcggca ctttgcatcc gccgctctcc
     4801 ctattcccga agtgcttgac attggggagt tcagcgagag cctgacctat tgcatctccc
     4861 gccgtgcaca gggtgtcacc ttgcaagacc tgcctgaaac cgaactgccc gctgttctcc
     4921 agcccgtcgc cgaggccatg gatgccatcg ctgccgccga tcttagccag accagcgggt
     4981 tcggcccatt cggacctcaa ggaatcggtc aatacactac atggcgcgat ttcatctgcg
     5041 ctattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcct
     5101 ccgtcgccca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     5161 acctcgtgca cgccgatttc ggctccaaca atgtcctgac cgacaatggc cgcataacag
     5221 ccgtcattga ctggagcgag gccatgttcg gggattccca atacgaggtc gccaacatct
     5281 tcttctggag gccctggttg gcttgtatgg agcagcagac ccgctacttc gagcggaggc
     5341 atcccgagct tgcaggatct cctcggctcc gggcttatat gctccgcatt ggtcttgacc
     5401 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggct cagggtcgct
     5461 gcgacgcaat cgtccggtcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     5521 gcgctgccgt ctggaccgat ggctgtgtgg aagtgctcgc cgatagtgga aacagacgcc
     5581 ccagcactcg tcctagggca aaggatctgc agtaaacgcg ttaagtcgac aatcaacctc
     5641 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5701 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5761 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5821 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5881 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5941 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6001 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6061 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6121 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6181 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     6241 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6301 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6361 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6421 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6481 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     6541 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6601 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6661 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6721 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6781 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6841 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6901 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6961 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7021 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     7081 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7141 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7201 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7261 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7321 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7381 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7441 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7501 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7561 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7621 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7681 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7741 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7801 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7861 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7921 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7981 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8041 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8101 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8161 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8221 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8281 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8341 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8401 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8461 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8521 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8581 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8641 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8701 agaaaagatc aaaggatctt c
//
