LOCUS       sequence-467900-        8764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a EnAsCas12a control guide that cuts an intergenic 
            region on chromosome 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Borck PC, Boyle I, Jankovic K, Bick N, Foster K, Lau AC, 
            Parker-Burns LI, Lubicki DA, Li T, Borah AA, Lofaso NJ, Das Sharma 
            S, Chan T, Kishen RV, Adeagbo A, Raghavan S, Aquilanti E, Prensner 
            JR, Krill-Burger JM, Golub TR, Campbell CD, Dempster JM, Chan EM, 
            Vazquez F
  TITLE     SKI complex loss renders 9p21.3-deleted or MSI-H cancers dependent 
            on PELO.
  JOURNAL   Nature. 2025 Feb 5. doi: 10.1038/s41586-024-08509-3.
  PUBMED    39910293
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Feb 5. doi: 10.1038/s41586-024-08509-3."
COMMENT     Sequence Label: pRDA052H_sgCh2
FEATURES             Location/Qualifiers
     source          1..8764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2776..3024
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2776..2796
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2947..2966
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    3180..3297
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3436..4614
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3667..4605
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4562..4582
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4627..5646
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    5674..6262
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5727..5747)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6145..6156)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6334..6567
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6645..6766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6682..6701)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6736..6755
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6806..6941
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6961..6980)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6962..6980)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6990..7007)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6990..7006)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6999..7021)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7148..7603
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7235..7254)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7445..7466
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7629..7733
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7734..7802,7803..8594)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7952..7971)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccagag gtaccgaggg cctatttccc atgattcctt catatttgca tatacgatac
     2821 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
     2881 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     2941 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     3001 atcttgtgga aaggacgaaa caccggtaat ttctactctt gtagatgtaa aaccctacta
     3061 atattccact aatttctact atcgtagatg cactgcaggt gtcatgatga agttttttga
     3121 attcgctagc aagcttggcg taactagatc ttgagacaaa tggcagtatt catccacaat
     3181 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     3241 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     3301 gtttattaca gggacagcag agatccactt tggcgccggc tcgagggggc ccgggtgcaa
     3361 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
     3421 aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     3481 cccgagaagt tggggggagg ggtcggcaat tgatccggtg cctagagaag gtggcgcggg
     3541 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3601 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3661 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3721 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3781 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3841 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3901 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3961 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     4021 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     4081 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     4141 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     4201 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     4261 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     4321 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     4381 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     4441 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     4501 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4561 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgacgtacg
     4621 gccaccatga aaaagcctga actcaccgct acctctgtcg agaagtttct gatcgaaaag
     4681 ttcgacagcg tatccgacct gatgcagctc tccgagggcg aagaatctcg ggctttcagc
     4741 ttcgatgtgg gagggcgtgg atatgtcctg cgggtgaata gctgcgccga tggtttctac
     4801 aaagatcgct atgtttatcg gcactttgca tccgccgctc tccctattcc cgaagtgctt
     4861 gacattgggg agttcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     4921 accttgcaag acctgcctga aaccgaactg cccgctgttc tccagcccgt cgccgaggcc
     4981 atggatgcca tcgctgccgc cgatcttagc cagaccagcg ggttcggccc attcggacct
     5041 caaggaatcg gtcaatacac tacatggcgc gatttcatct gcgctattgc tgatccccat
     5101 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cctccgtcgc ccaggctctc
     5161 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgccgat
     5221 ttcggctcca acaatgtcct gaccgacaat ggccgcataa cagccgtcat tgactggagc
     5281 gaggccatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccctgg
     5341 ttggcttgta tggagcagca gacccgctac ttcgagcgga ggcatcccga gcttgcagga
     5401 tctcctcggc tccgggctta tatgctccgc attggtcttg accaactcta tcagagcttg
     5461 gttgacggca atttcgatga tgcagcttgg gctcagggtc gctgcgacgc aatcgtccgg
     5521 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgctgc cgtctggacc
     5581 gatggctgtg tggaagtgct cgccgatagt ggaaacagac gccccagcac tcgtcctagg
     5641 gcaaaggatc tgcagtaaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     5701 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5761 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5821 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5881 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5941 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6001 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6061 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6121 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6181 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6241 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     6301 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6361 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6421 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6481 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6541 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     6601 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     6661 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6721 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     6781 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6841 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6901 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     6961 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     7021 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7081 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7141 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7201 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7261 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7321 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7381 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7441 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7501 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7561 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     7621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7741 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7801 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7861 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7921 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7981 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8041 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8101 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8161 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8221 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8281 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8341 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8401 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8461 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8521 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8581 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8641 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8761 cttc
//
