LOCUS       sequence-467904-       10006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of wild-type human PELO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Borck PC, Boyle I, Jankovic K, Bick N, Foster K, Lau AC, 
            Parker-Burns LI, Lubicki DA, Li T, Borah AA, Lofaso NJ, Das Sharma 
            S, Chan T, Kishen RV, Adeagbo A, Raghavan S, Aquilanti E, Prensner 
            JR, Krill-Burger JM, Golub TR, Campbell CD, Dempster JM, Chan EM, 
            Vazquez F
  TITLE     SKI complex loss renders 9p21.3-deleted or MSI-H cancers dependent 
            on PELO.
  JOURNAL   Nature. 2025 Feb 5. doi: 10.1038/s41586-024-08509-3.
  PUBMED    39910293
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Feb 5. doi: 10.1038/s41586-024-08509-3."
COMMENT     Sequence Label: pLX313_PELO_WT
FEATURES             Location/Qualifiers
     source          1..10006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2823..3033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2845..3041
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2892..3027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3063..4082
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    4153..4270
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4408..5586
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4639..5577
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5534..5554
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5606..5630
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6792..6816)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6824..6865
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6916..7504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6969..6989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7387..7398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7576..7809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7887..8008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7924..7943)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7978..7997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8048..8183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8203..8222)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8204..8222)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8232..8249)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8232..8248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8241..8263)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8390..8845
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8477..8496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8687..8708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8871..8975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8976..9044,9045..9836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9194..9213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     3061 ccatgaaaaa gcctgaactc accgctacct ctgtcgagaa gtttctgatc gaaaagttcg
     3121 acagcgtctc cgacctgatg cagctctccg agggcgaaga atctcgggct ttcagcttcg
     3181 atgtgggagg gcgtggatat gtcctgcggg tgaatagctg cgccgatggt ttctacaaag
     3241 atcgctatgt ttatcggcac tttgcatccg ccgctctccc tattcccgaa gtgcttgaca
     3301 ttggggagtt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacct
     3361 tgcaagacct gcctgaaacc gaactgcccg ctgttctcca gcccgtcgcc gaggccatgg
     3421 atgccatcgc tgccgccgat cttagccaga ccagcgggtt cggcccattc ggacctcaag
     3481 gaatcggtca atacactaca tggcgcgatt tcatctgcgc tattgctgat ccccatgtgt
     3541 atcactggca aactgtgatg gacgacaccg tcagtgcctc cgtcgcccag gctctcgatg
     3601 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gccgatttcg
     3661 gctccaacaa tgtcctgacc gacaatggcc gcataacagc cgtcattgac tggagcgagg
     3721 ccatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccctggttgg
     3781 cttgtatgga gcagcagacc cgctacttcg agcggaggca tcccgagctt gcaggatctc
     3841 ctcggctccg ggcttatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     3901 acggcaattt cgatgatgca gcttgggctc agggtcgctg cgacgcaatc gtccggtccg
     3961 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgctgccgtc tggaccgatg
     4021 gctgtgtgga agtgctcgcc gatagtggaa acagacgccc cagcactcgt cctagggcaa
     4081 aggatctgca gtaatgaggc gcgccgttaa cgaattctag atcttgagac aaatggcagt
     4141 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     4201 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     4261 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggc
     4321 ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
     4381 ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
     4441 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     4501 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     4561 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     4621 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     4681 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     4741 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     4801 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     4861 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     4921 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     4981 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     5041 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     5101 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     5161 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     5221 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     5281 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     5341 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     5401 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     5461 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     5521 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     5581 tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca ccatgaagct
     5641 cgtgaggaag aacatcgaga aggacaatgc gggccaggtg accctggtcc ccgaggagcc
     5701 tgaggacatg tggcacactt acaacctcgt gcaggtgggc gacagcctgc gcgcctccac
     5761 catccgcaag gtacagacag agtcctccac gggcagcgtg ggcagcaacc gggtccgcac
     5821 taccctcact ctctgcgtgg aggccatcga cttcgactct caagcctgcc agctgcgggt
     5881 taaggggacc aacatccaag agaatgagta tgtcaagatg ggggcttacc acaccatcga
     5941 gctggagccc aaccgccagt tcaccctggc caagaagcag tgggatagtg tggtactgga
     6001 gcgcatcgag caggcctgtg acccagcctg gagcgctgat gtggcggctg tggtcatgca
     6061 ggaaggcctc gcccatatct gcttagtcac tcccagcatg accctcactc gggccaaggt
     6121 ggaggtgaac atccctagga aaaggaaagg caattgctct cagcatgacc gggccttgga
     6181 gcggttctat gaacaggttg tccaggctat ccagcgccac atacactttg atgttgtaaa
     6241 gtgcatcctg gtggccagcc caggatttgt gagggagcag ttctgcgact acatgtttca
     6301 acaagcagtg aagaccgaca acaaactgct cctggaaaac cggtccaaat ttcttcaggt
     6361 acatgcctcc tccggacaca agtactccct gaaagaggcc ctttgtgacc ctactgtggc
     6421 tagccgcctt tcagacacta aagctgctgg ggaagtcaaa gccttggatg acttctataa
     6481 aatgttacag catgaaccgg atcgagcttt ctatggactc aagcaggtgg agaaggccaa
     6541 tgaagccatg gcaattgaca cattgctcat cagcgatgag ctcttcaggc atcaggatgt
     6601 agccacacgg agccggtatg tgaggctggt ggacagtgtg aaagagaatg caggcaccgt
     6661 taggatattc tctagtcttc acgtttctgg ggaacagctc agccagttga ctggggtagc
     6721 tgccattctc cgcttccctg ttcccgaact ttctgaccaa gagggtgatt ccagttctga
     6781 agaggattaa ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa
     6841 ccctctcctc ggtctcgatt ctacgtagta atgaactagt accggttaag tcgacaatca
     6901 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6961 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7021 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7081 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7141 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7201 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7261 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     7321 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7381 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7441 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7501 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7561 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     7621 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7681 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7741 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7801 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7861 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7921 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7981 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     8041 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8101 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8161 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     8221 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8281 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8341 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     8401 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8461 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8521 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8581 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8641 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8701 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8761 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8821 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8881 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8941 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9001 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9061 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9121 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9181 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9241 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9301 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9361 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9421 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9481 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9541 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9601 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9661 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9721 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9781 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9841 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9901 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
