LOCUS       sequence-467905-       13332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dCas9-KRAB and sgRNA targeting PELO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13332)
  AUTHORS   Borck PC, Boyle I, Jankovic K, Bick N, Foster K, Lau AC, 
            Parker-Burns LI, Lubicki DA, Li T, Borah AA, Lofaso NJ, Das Sharma 
            S, Chan T, Kishen RV, Adeagbo A, Raghavan S, Aquilanti E, Prensner 
            JR, Krill-Burger JM, Golub TR, Campbell CD, Dempster JM, Chan EM, 
            Vazquez F
  TITLE     SKI complex loss renders 9p21.3-deleted or MSI-H cancers dependent 
            on PELO.
  JOURNAL   Nature. 2025 Feb 5. doi: 10.1038/s41586-024-08509-3.
  PUBMED    39910293
REFERENCE   2  (bases 1 to 13332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Feb 5. doi: 10.1038/s41586-024-08509-3."
COMMENT     Sequence Label: pXPR_023d sgCiPELO #1
FEATURES             Location/Qualifiers
     source          1..13332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2852..3085
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3157..3745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3263..3274
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3672..3692
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3761..4357)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3844..3864)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(4358..4414)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4457..4477)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4484..4504)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4517..4543)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(4523..4549)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(join(4553..6136,6137..6139,6140..8626,8627..8629,
                     8630..8656))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4553..8656)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(8678..8863)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     protein_bind    complement(8893..8917)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(8943..9154)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(9216..9291)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(9313..9561)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(9371..9390)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(9541..9561)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    9612..9729
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10027..10071)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10256..10489
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10982..11107
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             11154..11334
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(11352..11550)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(11377..11397)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        11552..11931
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     12103..12122
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        12197..12301
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12302..12370,12371..13162)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12520..12539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac tgactaaaag
     2881 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     2941 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     3001 caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt
     3061 cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct
     3121 acagctgcct tgtaagtcat tggtcttaaa gtcgacgcgg ggaggcggcc caaagggaga
     3181 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     3241 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     3301 gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca
     3361 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     3421 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     3481 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     3541 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     3601 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     3661 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     3721 aaattttgta atccagaggt tgattgtcga cttaacgcgt tcaggcaccg ggcttgcggg
     3781 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
     3841 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
     3901 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
     3961 ggtcgggcga gactccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
     4021 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
     4081 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
     4141 ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
     4201 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc
     4261 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt
     4321 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcgg tccaggattc tcttcgacat
     4381 ctccggcttg tttcagcaga gagaagtttg ttgcgccgga tccgctgctg ccgttgctcc
     4441 cactaccaat gccgtccact ttccgctttt tcttaggatc ttccaccttg cgttttttct
     4501 tgggggagcc gctgcccagg ctggcataat cgggcacgtc atagggatag gcgtcgcctc
     4561 ccagctgaga caggtcgatc cgtgtctcgt acaggccggt gatgctctgg tggatcaggg
     4621 tggcgtccag cacctctttg gtgctggtgt acctcttccg gtcgatggtg gtgtcaaagt
     4681 acttgaaggc ggcaggggct cccagattgg tcagggtaaa caggtggatg atattctcgg
     4741 cctgctctct gataggcttg tctctgtgct tgttgtaggc gctcagcacc ttgtccagat
     4801 tagcgtcggc caggatcact ctcttggaga actcgctgat ctgctcgatg atctcgtcca
     4861 ggtagtgttt gtgctgttcc acaaacagct gtttctgctc attatcctcg ggggagccct
     4921 tcagcttctc atagtggctg gccaggtaca ggaagttcac atatttggag ggcagggcca
     4981 gttcgtttcc cttctgcagt tcgccggcag aggccagcat tctcttccgg ccgttttcca
     5041 gctcgaacag ggagtactta ggcagcttga tgatcaggtc ctttttcact tctttgtagc
     5101 ccttggcttc cagaaagtcg atgggattct tctcgaagct gcttctttcc atgatggtga
     5161 tccccagcag ctctttcaca ctcttcagtt tcttggactt gcccttttcc actttggcca
     5221 ccaccagcac agaataggcc acggtggggc tgtcgaagcc gccgtacttc ttagggtccc
     5281 agtccttctt tctggcgatc agcttgtcgc tgttcctctt gggcaggata gactctttgc
     5341 tgaagccgcc tgtctgcacc tcggtctttt tcacgatatt cacttggggc atagacagca
     5401 ctttccgcac ggtggcaaag tcccggccct tatcccacac gatctcgcct gtttcgccgt
     5461 ttgtctcgat cagaggccgc ttccggatct cgccgttggc cagggtaatc tcggtcttga
     5521 aaaagttcat gatgttgctg tagaagaagt acttggcggt agccttgccg atttcctgct
     5581 cgctcttggc gatcatcttc cgcacgtcgt acaccttgta gtcgccgtac acgaactcgc
     5641 tttccagctt agggtacttt ttgatcaggg cggttcccac gacggcgttc aggtaggcgt
     5701 cgtgggcgtg gtggtagttg ttgatctcgc gcactttgta aaactggaaa tccttccgga
     5761 aatcggacac cagcttggac ttcagggtga tcactttcac ttcccggatc agtttgtcgt
     5821 tctcgtcgta cttagtgttc atccgggagt ccaggatctg tgccacgtgc tttgtgatct
     5881 gccgggtttc caccagctgt ctcttgatga agccggcctt atccagttcg ctcaggccgc
     5941 ctctctcggc cttggtcaga ttgtcgaact tcctctgggt aatcagcttg gcattcagca
     6001 gctggcgcca gtagttcttc atcttcttca cgacctcttc ggagggcacg ttgtcgctct
     6061 tgccccggtt cttgtcgctc cgagtcagca ctttgttatc gatggagtcg tccttcagaa
     6121 agctctgagg cacgatagcg tccacatcgt agtcggacag ccggttgatg tccagttcct
     6181 ggtccacgta catatcccgc ccattctgca ggtagtacag gtacagcttc tcgttctgca
     6241 gctgggtgtt ttccacgggg tgttctttca ggatctggct gcccagctct ttgatgccct
     6301 cttcgatccg cttcattctc tcgcggctgt tcttctgtcc cttctgggtg gtctggttct
     6361 ctctggccat ttcgatcacg atgttctcgg gcttgtgccg gcccatcact ttcacgagct
     6421 cgtccaccac cttcactgtc tgcaggatgc ccttcttaat ggcggggctg ccggccagat
     6481 tggcaatgtg ctcgtgcagg ctatcgccct ggccggacac ctgggctttc tggatgtcct
     6541 ctttaaaggt caggctgtcg tcgtggatca gctgcatgaa gtttctgttg gcgaagccgt
     6601 cggacttcag gaaatccagg attgtcttgc cggactgctt gtcccggatg ccgttgatca
     6661 gcttccggct cagcctgccc cagccggtgt atctccgccg cttcagctgc ttcatcactt
     6721 tgtcgtcgaa caggtgggca taggttttca gccgttcctc gatcatctct ctgtcctcaa
     6781 acagtgtcag ggtcagcacg atatcttcca gaatgtcctc gttttcctca ttgtccagga
     6841 agtccttgtc cttgataatt ttcagcagat cgtggtatgt gcccagggag gcgttgaacc
     6901 gatcttccac gccggagatt tccacggagt cgaagcactc gattttcttg aagtagtcct
     6961 ctttcagctg cttcacggtc actttccggt tggtcttgaa cagcaggtcc acgatggctt
     7021 ttttctgctc gccgctcagg aaggcgggct ttctcattcc ctcggtcacg tatttcactt
     7081 tggtcagctc gttgtacacg gtgaagtact cgtacagcag gctgtgcttg ggcagcacct
     7141 tctcgttggg caggttctta tcgaagttgg tcatccgctc gatgaagctc tgggcgctgg
     7201 cgcccttgtc caccacttcc tcgaagttcc agggggtgat ggtttcctcg ctctttctgg
     7261 tcatccaggc gaatctgctg tttcccctgg ccagagggcc cacgtagtag gggatgcgga
     7321 aggtcaggat cttctcgatc ttttcccggt tgtccttcag gaatgggtaa aaatcttcct
     7381 gccgccgcag aatggcgtgc agctctccca ggtggatctg gtgggggatg ctgccgttgt
     7441 cgaaggtccg ctgcttccgc agcaggtcct ctctgttcag cttcacgagc agttcctcgg
     7501 tgccgtccat cttttccagg atgggcttga tgaacttgta gaactcttcc tggctggctc
     7561 cgccatcgat gtagccggcg tagccgttct tgctctggtc gaagaaaatc tctttgtact
     7621 tctcaggcag ctgctgccgc acgagagctt tcagcagggt caggtcctgg tggtgctcgt
     7681 cgtatctctt gatcatagag gcgctcaggg gggccttggt gatctcggtg ttcactctca
     7741 ggatgtcgct cagcaggatg gcgtcggaca ggttcttggc ggccagaaac aggtcggcgt
     7801 actggtcgcc gatctgggcc agcaggttgt ccaggtcgtc gtcgtaggtg tccttgctca
     7861 gctgcagttt ggcatcctcg gccaggtcga agttgctctt gaagttgggg gtcaggccca
     7921 ggctcagggc aatcaggttg ccgaacaggc cattcttctt ctcgccgggc agctgggcga
     7981 tcagattttc cagccgtctg ctcttgctca gtctggcaga caggatggcc ttggcgtcca
     8041 cgccgctggc gttgatgggg ttttcctcga acagctggtt gtaggtctgc accagctgga
     8101 tgaacagctt gtccacgtcg ctgttgtcgg ggttcaggtc gccctcgatc aggaagtggc
     8161 cccggaactt gatcatgtgg gccagggcca gatagatcag ccgcaggtcg gccttgtcgg
     8221 tgctgtccac cagtttcttt ctcaggtggt agatggtggg gtacttctcg tggtaggcca
     8281 cctcgtccac gatgttgccg aagatggggt gccgctcgtg cttcttatcc tcttccacca
     8341 ggaaggactc ttccagtctg tggaagaagc tgtcgtccac cttggccatc tcgttgctga
     8401 agatctcttg cagatagcag atccggttct tccgtctggt gtatcttctt ctggcggttc
     8461 tcttcagccg ggtggcctcg gctgtttctc cgctgtcgaa cagcagggcg ccgatcaggt
     8521 tcttcttgat gctgtgccgg tcggtgttgc ccagcacctt gaatttcttg ctgggcacct
     8581 tgtactcgtc ggtgatcacg gcccagccca cagagttggt gccgatggcc aggccgatgc
     8641 tgtacttctt gtccatcgac cctccgccgg aaccgccggg ctcttctccc ttctccaacc
     8701 ggaggatcac atctggctta gtaagctgat aacccaagga aaccaggttc ttatagttct
     8761 ccagcatcac atttctgtac acgatctgct gagcagtgtc cagcagcttc cactcctccc
     8821 tggtgaagtc cacaaataca tccttgaagg tcaccagtgt ccgggaccag gcagttagtg
     8881 acttagcatc catgccaact tttttgtaca aacttgttga tcaatcgatg ctagaaccgg
     8941 tcctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
     9001 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
     9061 cagtttaccc cgcgccacct tctctaggca ccggatcaat tgccgacccc tccccccaac
     9121 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gccaattccc
     9181 actcctttca agacctagct agcgaattca aaaaagcacc gactcggtgc cactttttca
     9241 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa ccgagcctgt
     9301 tagacgcagc gcggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata
     9361 ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa
     9421 ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac
     9481 agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca aatatgaagg
     9541 aatcatggga aataggccct cggtacctta attaaccaaa ctggatctct gctgtccctg
     9601 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     9661 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     9721 cttttaaaat tgtggatgaa tactgccatt tgtctgcaga attggcgcac gcagtgccga
     9781 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
     9841 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     9901 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     9961 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
    10021 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
    10081 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
    10141 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
    10201 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
    10261 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
    10321 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
    10381 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
    10441 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
    10501 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
    10561 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
    10621 tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
    10681 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
    10741 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
    10801 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
    10861 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
    10921 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
    10981 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
    11041 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
    11101 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
    11161 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
    11221 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
    11281 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
    11341 ggcaaaacgc gctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
    11401 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
    11461 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
    11521 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
    11581 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
    11641 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
    11701 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
    11761 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
    11821 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
    11881 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat
    11941 atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc
    12001 aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga
    12061 cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg
    12121 tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac
    12181 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    12241 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    12301 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    12361 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    12421 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    12481 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    12541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    12601 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    12661 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    12721 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    12781 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    12841 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    12901 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    12961 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    13021 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    13081 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    13141 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    13201 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    13261 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    13321 caaaggatct tc
//
