LOCUS       sequence-46794-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: YWHAG
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(57..77)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     RBS             826..848
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 acttctttgg gcttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagggat
       61 tggaagtaca ggttctcatt gttgccttcg ccgccatcgt cgtcctgctg gtcgctcgtc
      121 cagagcgtga ggttgtcgcg gaggagctgc atgatgagcg tggagtcctt gtaggagtcc
      181 tcgttgaggg tgtcaagctc ggcgatggcg tcgtcgaacg cggtcttggc caagtggcac
      241 gcttgctctg gggcgttctg gatctcatag tagaagacgg agtagttaag agccaggcct
      301 aatcggatgg ggtgggtggg ctgcatgtgc tctttgctga tctcgtgggc ttcgctgtag
      361 gccttctcag aggactccac caccgtcgcc cttttctctc cggtggccac ttcagccagg
      421 tagcggtagt agtccccttt catcttcagg tagaacactt tgctctcgta ctgggtctcg
      481 ctgcaattct tgatcaggta gttatccagc aggctcagca catcctggca cacagcctcc
      541 aactccttct ctatcttctc ccggtacgca cggaccatct caatcttctt ctcattgccg
      601 tctgcagatg tcttctgctc aatgctactg atgaccctcc aggaagagcg gcgtgccccc
      661 acaacgttct tgtaggccac agacagaagg tttcgttcct cattcgacag tggctcattc
      721 agctctgtca cgttcttcat ggccgcggcc atgtcgtcgt agcgctccgc ctgctcggcc
      781 agccgggctt tctgcaccag ttgctcgcgg tccaccatat gtatatctcc ttcttaaagt
      841 taaacaaaat tatttctaga ggggaattgt tatccgct
//
