LOCUS       sequence-467971-        2972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses photoactivable fluorescent protein for Mitofusin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2972)
  TITLE     Photoactivable plasmid labeling MFN1 for Mammalian expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PAmKate-MFN1
FEATURES             Location/Qualifiers
     source          1..2972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..732
                     /codon_start=1
                     /product="monomeric photoactivatable red fluorescent 
                     protein (Gunewardene et al., 2011)"
                     /label=PAmKate
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRGD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 ccgctagcac cggtcgccac catggtgtct aagggcgaag agctgattaa ggagaacatg
       61 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag
      121 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc
      181 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag caagaccttc
      241 atcaaccaca cccagggcat ccccgacttc tggaagcagt ccttccctga gggcttcaca
      301 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
      361 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac
      421 ggccctgtga tgcagaagaa aacactcggc tgggaggcca acaccgagat gctgtacccc
      481 gctgacggcg gcctggaagg cagaggggac atggccctga agctcgtggg cgggggccac
      541 ctgatctgca acttgaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg
      601 cccggcgtct actatgtgga ccgcagactg gaaagaatca aggaggccga caaagagacc
      661 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
      721 cacaagctta attcgaattc gatggcagaa cctgtttctc cactgaagca ctttgtgctg
      781 gctaagaagg cgattactgc aatctttgac cagttactgg agtttgttac tgaaggatca
      841 cattttgttg aagcaacata taagaatccg gaacttgatc gaatagccac tgaagatgat
      901 ctggtagaaa tgcaaggata taaagacaag ctttccatca ttggtgaggt gctatctcgg
      961 agacacatga aggtggcatt ttttggcagg acaagcagtg ggaagagctc tgttatcaat
     1021 gcaatgttgt gggataaagt tctccctagt gggattggcc atataaccaa ttgcttccta
     1081 agtgttgaag gaactgatgg agataaagcc tatcttatga cagaaggatc agatgaaaaa
     1141 aagagtgtga agacagttaa tcaactggcc catgcccttc acatggacaa agatttgaaa
     1201 gctggctgtc ttgtacgtgt gttttggcca aaagcaaaat gtgccctctt gagagatgac
     1261 ctggtgttag tagacagtcc aggcacagat gtcactacag agctggatag ctggattgat
     1321 aagttttgcc tagatgctga tgtctttgtt ttggtcgcaa actctgaatc aacactaatg
     1381 aatacggaaa aacacttttt tcacaaggtg aatgagcggc tttccaagcc taatattttc
     1441 attctcaata atcgttggga tgcctctgca tcagagccag aatatatgga agacgtacgc
     1501 agacagcaca tggaaagatg cctgcatttc ttggtggagg agctcaaagt tgtaaatgct
     1561 ttagaagcac agaatcgtat cttctttgtt tcagcaaagg aagttcttag tgctagaaag
     1621 caaaaagcac aggggatgcc agaaagtggt gtggcacttg ctgaaggatt tcatgcaaga
     1681 ttacaggaat ttcagaattt tgaacaaatc tttgaggagt gtatctcgca gtcagcagtg
     1741 aaaacaaagt tcgaacagca cactatcaga gctaaacaga tactagctac tgtgaaaaac
     1801 ataatggatt cagtaaacct ggcagctgaa gataaaaggc attattcagt ggaagagagg
     1861 gaagaccaaa ttgatagact ggactttatt cgaaaccaga tgaacctttt aacactggat
     1921 gttaagaaaa aaatcaagga ggttaccgag gaggtggcaa acaaagtttc atgtgcaatg
     1981 acagatgaaa tttgtcgact gtctgttttg gttgatgaat tttgttcaga gtttcatcct
     2041 aatccagatg tattaaaaat atataaaagt gaattaaata agcacataga ggatggtatg
     2101 ggaagaaatt tggctgatcg atgcaccgat gaagtaaacg ccttagtgct tcagacccag
     2161 caagaaatta ttgaaaattt gaagccatta cttccagctg gtatacagga taaactacat
     2221 acactgatcc cttgcaagaa atttgatctc agttataatc taaattacca caagttatgt
     2281 tcagattttc aagaggatat tgtatttcgt ttttccctgg gctggtcttc ccttgtacat
     2341 cgatttttgg gccctagaaa tgctcaaagg gtgctcctag gattatcaga gcctatcttt
     2401 cagctcccta gatctttagc ttctactccc actgctccta ccactccagc aacgccagat
     2461 aatgcatcac aggaagaact catgattaca ttagtaacag gattggcgtc cgttacatct
     2521 agaacttcta tgggcatcat tattgttgga ggagtgattt ggaaaactat aggctggaaa
     2581 ctcctatctg tttcattaac tatgtatgga gctttgtatc tttatgaaag actgagctgg
     2641 accacccatg ccaaggagcg agcctttaaa cagcagtttg taaactatgc aactgaaaaa
     2701 ctgaggatga ttgttagctc cacgagtgca aactgcagtc accaagtaaa acaacaaata
     2761 gctaccactt ttgctcgcct gtgccaacaa gttgatatta ctcaaaaaca gctggaagaa
     2821 gaaattgcta gattacccaa agaaatagat cagttggaga aaatacaaaa caattcaaag
     2881 ctcttaagaa ataaagctgt tcaacttgaa aatgagctgg agaattttac taagcagttt
     2941 ctaccttcaa gcaatgaaga atcctaagga tc
//
