LOCUS       sequence-467973-       13552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for genetically-encoded FRET biosensor for 
            Gibberellin (GA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13552)
  AUTHORS   Griffiths J, Rizza A, Tang B, Frommer WB, Jones AM
  TITLE     GIBBERELLIN PERCEPTION SENSOR 2 reveals genesis and role of cellular
            GA dynamics in light-regulated hypocotyl growth.
  JOURNAL   Plant Cell. 2024 Jul 23:koae198. doi: 10.1093/plcell/koae198.
  PUBMED    39039020
REFERENCE   2  (bases 1 to 13552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Jul 23:koae198. doi: 10.1093/plcell/koae198."
COMMENT     Sequence Label: p16 nlsGPS2
FEATURES             Location/Qualifiers
     source          1..13552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    125..149
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    314..335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(350..355,356..373,374..380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    388..404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     393..415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     412..428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     412..428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             965..985
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1697..1721
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3015..3039)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3053..3055,3056..3766)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALFKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     primer_bind     complement(3095..3116)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3356..3375)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3767..3796
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3864..4505
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(4513..4530)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4513..4529)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4522..4544)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(5111..5363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(5559..6353)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5670..5689)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6280..6299
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6383..6763)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    7135..7159
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7680..8471
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8717..9305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9206..9225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9459..9476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9491..9631
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9557..9576)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9717..9739
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9975..10169
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10235..11308)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11737..12366)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa
       61 cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc
      121 ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa
      181 cgacaatctg atccaagctc aagctgctct agccaatacg caaaccgcct ctccccgcgc
      241 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      301 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      361 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      421 gctatgacat gattacgaat tcgagctcgg tacctggaac catcttttgg gttcctttct
      481 tcttctcttt ttttgtttct ctcttaaaag tcttttctct tttcaagtgc ttcaaacaaa
      541 actaatttgt tataaaggga gtttctctat tttattatat agcaaaaaac ttccaaaatt
      601 tctcatttct gtgtttaacc ttttcgtaca tcagttttaa agcacagaga gctcaatgtt
      661 cttccaatat cgttattaat aaattttgat taaattcaat caaatcggag ttatattacc
      721 acatgaggtt aaagggccat attaaaaagt ctgcacttca tatgagcaac aaggctttta
      781 tgtctttatg gttgatttga tggcccatat atgatagttc aaaggcccat attaaaaaat
      841 gccctaacat gattcgattc gtatataaag acgcttaatg tgacaagcga acttgctcat
      901 tagggtttct catctacgac ggcgtggggt acccggggat cctctagagc tcatgatgct
      961 gcagcctaag aagaagagaa aggttggagg gctcgaggtt tctaaaggtg aagaattgtt
     1021 tacgggcgtc gtcccgatcc tcgtggaact cgacggggat gttaacgggc ataagttttc
     1081 ggtcagcggg gaaggggagg gggacgcgac gtatgggaag ctcactctca agctgatctg
     1141 tacgacgggg aaactcccgg tcccgtggcc gacgctggtc acgacgctgg gatacgggct
     1201 ccaatgcttt gcgaggtatc cggaccacat gaaacagcat gactttttca aatcggcgat
     1261 gccggaggga tacgtgcagg aacggacgat ctttttcaaa gacgatggga actataagac
     1321 gcgggcggaa gtcaagtttg aaggggacac gctcgtcaac cggatcgaac tcaaggggat
     1381 tgacttcaaa gaggatggga acatactcgg ccataagctc gaatacaatt acaactcgca
     1441 taacgtatac atcaccgcgg ataagcaaaa gaacgggatc aaagccaatt tcaaaatccg
     1501 gcataacata gaggatgggg gggtccaact ggcggatcac tatcagcaaa acacgccgat
     1561 aggggatggg ccggtcctcc tcccggataa ccattacctc tcgtaccaaa gcgcgctctt
     1621 caaggacccg aatgagaaac gggaccacat ggttctcctg gagttcctca cggcggcggg
     1681 cataggtacc gatatcacaa gtttgtacaa aaaagcaggc ttcgatgagc ttctagctgt
     1741 tcttggttac aaggttaggt catccgaaat ggctgatgtt gctcagaaac tcaagcagct
     1801 tagagttatg atgtctaatg ttcaagaaga cgatctttct caactcgcta ctgagactgt
     1861 tcactataat ccggcggagc tttacacgtg gcttgattct atgctcaccg accttaatcc
     1921 tccgtcgtct aacgccgagt acgatgcaag tggcgcgcct ggggccggcc gaagttctgg
     1981 aatggctgga agtgaagaag ttaatcttat tgagagcgag acagtggttc ctctcaatac
     2041 atgggttcta atatccaact ttgagctagc ttacaatctc ctgcgtcgcc ctgacggaac
     2101 ttttaaccgt catctcgcag agtttctaga ccggaaagtc cctgcaaatg ccaaccctgt
     2161 taatggggtc ttctcttttg atgtgatcat cgatcgccaa actaatttgc ttagcagagt
     2221 ttacagacca gctgatgctg gcacttcacc aagtattact gatctacaga atcctgttga
     2281 tggtgaaata gtgcctgtta ttgtcttctt tcatggtgga agctttgcac attcttctgc
     2341 aaacagtgct atttatgata ctctttgtcg taggcttgtt ggtttgtgtg gtgctgttgt
     2401 tgtctctgtg aattatcgtc gtgcaccaga gaatcgatac ccttgtgctt atgatgatgg
     2461 atgggctgtt ttgaaatggg tcaactcgag ttcttggctt agaagcaaga aagactcaaa
     2521 ggttcgtatt ttcttggcgg gtgatagctc tgggggtaac attgtgcata atgtcgcggt
     2581 aagagcggtt gagtcaagga tcgatgtttt ggggaacatt ttgcttaacc ctatgtttgg
     2641 agggaccgaa agaacggaat ctgagaaacg tttggatggg aagtactttg ttacggttag
     2701 agaccgagat tggtattgga gagcgtttct tcctgagggt gaagacagag agcatccagc
     2761 gtgtagcccg tttggcccga gaagcaagag tctagaaggg ttgagtttcc ccaagagtct
     2821 tgtcgttgta gcgggtttag atttgattca agattggcaa ttgaagtacg cggaagggct
     2881 caagaaagcg ggtcaagagg tgaagcttct ttacttggag caagccacta ttggcttcta
     2941 cttattgcct aacaacaatc acttccatac cgttatggat gagatagctg catttgtaaa
     3001 cgcagaatgc caacacccag ctttcttgta caaagtggtg atatcgacta gtgtgagcaa
     3061 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3181 cctgaagttc atctgcacca ccggtaagct gcccgtgccc tggcccaccc tcgtgaccac
     3241 cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     3301 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3361 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3421 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3481 caacgccatc agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
     3541 caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3601 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3661 ccagtccgcc ctgttcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3721 cctgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggaac aaaaattgat
     3781 aagtgaggaa gatttataag ggcccgggac gtccgcggag atctacgcgt gtcgactcga
     3841 gatatccaac tagttggctg cagagctttc gttcgtatca tcggtttcga caacgttcgt
     3901 caagttcaat gcatcagttt cattgcgcac acaccagaat cctactgagt ttgagtatta
     3961 tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt actgtgtttt
     4021 ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag ttaatgaatg
     4081 atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt atttgttgtg
     4141 tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa aatgtgtcaa
     4201 atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acacttgtag ttgtaccatt
     4261 atgcttattc actaggcaac aaatatattt tcagacctag aaaagctgca aatgttactg
     4321 aatacaagta tgtcctcttg tgttttagac atttatgaac tttcctttat gtaattttcc
     4381 agaatccttg tcagattcta atcattgctt tataattata gttatactca tggatttgta
     4441 gttgagtatg aaaatatttt ttaatgcatt ttatgacttg ccaattgatt gacaacatgc
     4501 atcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4561 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4621 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt
     4681 gccaatcgag gccgaccaac cgcaagcgtt gtcagtgttg caaagcgctc tgtgtgggcc
     4741 tactttaatt gcttccagtg ttaaattggc gaaaggcaat aatatcgcaa aatattgtgt
     4801 tgtaaaatgt aattatgttt taatttcatg gaaatgtttg agcataattt ttattaatgt
     4861 actaaattac tgttttgtta aatgcaattt tgctttctcg ggattttaat atcaaaatct
     4921 atttagaaat acacaatatt ttgttgcagg cttgctggag aatcgatctg ctatcataaa
     4981 aattacaaaa aaattttatt tgcctcaatt attttaggat tggtattaag gacgcttaaa
     5041 ttatttgtcg ggtcactacg catcattgtg attgagaaga tcagcgatac gaaatattcg
     5101 tagtactatc gataatttat ttgaaaattc ataagaaaag caaacgttac atgaattgat
     5161 gaaacaatac aaagacagat aaagccacgc acatttagga tattggccga gattactgaa
     5221 tattgagtaa gatcacggaa tttctgacag gagcatgtct tcaattcagc ccaaatggca
     5281 gttgaaatac tcaaaccgcc ccatatgcag gagcggatca ttcattgttt gtttggttgc
     5341 ctttgccaac atgggagtcc aaggtttcag ggaagctgga attccggggt gggcgaagaa
     5401 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     5461 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     5521 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     5581 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     5641 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     5701 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     5761 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     5821 atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     5881 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     5941 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     6001 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     6061 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     6121 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctggagttca
     6181 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     6241 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     6301 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     6361 gatccgggga attcgctaga gtcgatttgg tgtatcgaga ttggttatga aattcagatg
     6421 ctagtgtaat gtattggtaa tttgggaaga tataatagga agcaaggcta tttatccatt
     6481 tctgaaaagg cgaaatggcg tcaccgcgag cgtcacgcgc attccgttct tgctgtaaag
     6541 cgttgtttgg tacacttttg actagcgagg cttggcgtgt cagcgtatct attcaaaagt
     6601 cgttaatggc tgcggatcaa gaaaaagttg gaatagaaac agaatacccg cgaaattcag
     6661 gcccggttgc catgtcctac acgccgaaat aaacgaccaa attagtagaa aaataaaaac
     6721 tgactcggat acttacgtca cgtcttgcgc actgatttga aaaatctcaa tataaacaaa
     6781 gacggccaca agaaaaaacc aaaacaccga tattcattaa tcttatctag tttctcaaaa
     6841 aaattcatat cttccacacg tgaaaatgcc aatttctcag acctacctcg gctctgcgaa
     6901 ggcccccgct ggtatcaaaa gtttttattt catccgacat ggcgcgaccg acctcaacga
     6961 gaaggaaatt gtcgtgaacg gtgagaagct ctggggcgtg caaggttccg gaacgaacat
     7021 cggtctcaat gcaaaagggg aacgccaggc tctgttggcc cctcgaaatt cggcgttaat
     7081 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     7141 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     7201 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     7261 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     7321 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     7381 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta
     7441 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     7501 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     7561 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     7621 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     7681 tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     7741 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     7801 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     7861 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     7921 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     7981 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     8041 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     8101 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     8161 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     8221 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     8281 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     8341 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     8401 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     8461 tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     8521 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     8581 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
     8641 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8701 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8761 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8821 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     8881 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8941 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9001 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9061 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9361 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9421 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9481 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     9541 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     9601 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     9661 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     9721 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     9781 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     9841 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     9901 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     9961 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
    10021 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
    10081 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
    10141 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
    10201 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
    10261 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
    10321 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
    10381 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
    10441 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
    10501 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
    10561 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
    10621 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
    10681 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
    10741 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
    10801 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
    10861 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
    10921 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
    10981 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
    11041 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
    11101 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
    11161 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
    11221 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
    11281 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
    11341 attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
    11401 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
    11461 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
    11521 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
    11581 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
    11641 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
    11701 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
    11761 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
    11821 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
    11881 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
    11941 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
    12001 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
    12061 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    12121 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    12181 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    12241 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    12301 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    12361 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    12421 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    12481 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    12541 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    12601 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    12661 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
    12721 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
    12781 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
    12841 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
    12901 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
    12961 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
    13021 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
    13081 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
    13141 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
    13201 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
    13261 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
    13321 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
    13381 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
    13441 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
    13501 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ct
//
