LOCUS       sequence-467984-        4557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF251 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF251
FEATURES             Location/Qualifiers
     source          1..4557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(149..176)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(268..354)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     403..425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     417..434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     418..434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    450..549
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2563..2662)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2679..2698)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2680..2698)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2821..2840)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2832..3641
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2899..2918)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3788..4376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4277..4296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4530..4547
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
      121 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
      181 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
      241 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
      301 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
      361 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
      421 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      481 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      541 aagttggcat ggccgccacc tttcagctgc ccggccacca ggagatgccc ctgaccttcc
      601 aggacgtggc tgtgtacttt agccaggctg agggaagaca gctgggccct cagcagagag
      661 ccctgtacag agacgtgatg ctggagaact acggcaacgt ggctagcctg ggatttcccg
      721 tgcccaagcc cgagctgatc tcccagctgg agcagggcaa agagctttgg gtgctgaacc
      781 tgctgggcgc cgaggagccc gacatcctga agagctgcca gaaggacagc gaggtgggca
      841 ccaagaagga gctgtctatc ctgaaccaga agttcagcga ggaggtgaag acccccgagt
      901 tcgtgagcag aagactgctg agagacaacg cccaggccgc cgagttcaga gaggcctggg
      961 gcagagaggg caagctgaag gagagagtgg gcaacagcgc cggccagagc ctgaacaagc
     1021 ccaacatcca caagagagtg ctgaccgagg ctaccgtggg cagggagcgc tctctgggcg
     1081 agagaacaca ggagtgcagc gccttcgaca gaaacctgaa tctggaccag aacgtggtga
     1141 gactgcagag aaacaagaca ggcgaacggg tgttcaagtg tgacatctgt agcaagacct
     1201 tcaagtacaa cagcgacctg agcagacacc agagatcaca caccggcgaa aagccatacg
     1261 agtgcggccg gtgcggaaga gcctttaccc acagcagcaa cctggtgctg caccaccaca
     1321 tccataccgg caacaaacct ttcaagtgcg acgagtgtgg caagacattc ggactgaaca
     1381 gccacctgag gctgcacaga cgcattcaca ccggtgagaa gccattcggc tgcggagagt
     1441 gtgggaaggc ctttagccgg agctctaccc tgattcagca cagaatcatt cataccggag
     1501 aaaaaccata caaatgcaat gaatgcggca gaggcttcag tcagagcccc cagctcacac
     1561 agcatcagag aattcacact ggagaaaagc ctcacgagtg ctcccactgc gggaaggctt
     1621 ttagcaggag cagctctctg atccagcatg agaggatcca caccggagag aagccccaca
     1681 agtgcaacca gtgcggcaag gcattcagcc agtcctccag cctgttcctg catcaccgcg
     1741 tgcacaccgg ggagaaacct tacgtctgca atgagtgcgg gagagccttc ggcttcaact
     1801 cccacctgac agagcacgtg agaatccata caggcgagaa gccttacgtg tgcaacgaat
     1861 gcggaaaggc tttcagaaga agctcaaccc tggtgcaaca cagaagggtg cacactgggg
     1921 agaagcccta tcagtgcgtg gaatgtggca aagctttttc tcagagcagc cagctgacac
     1981 tgcaccagag ggtccacact ggtgaaaagc cctacgactg cggcgactgt ggaaaagcat
     2041 ttagcagaag gtccactctc atccagcacc agaaggtgca ctctggcgaa accagaaagt
     2101 gcagaaagca cggccccgcc ttcgtgcacg gctctagcct gaccgccgac ggacagatcc
     2161 ccactggcga gaaacacggc agagctttta accacggcgc caacctgatc ctgagatgga
     2221 cagtgcacac aggggaaaag tccttcggat gcaacgagta tggcaaggct ttctctccca
     2281 ccagcaggcc caccgaggac cagatcatgc acgcaggcga aaaaccctac aagtgccagg
     2341 agtgtggaaa cgccttcagc ggcaagagca ccctgatcca acaccaggtg acccacacag
     2401 gccagaaacc ctgccactgc agcgtgtatg gaaaggcatt ttcccagagc tctcagctga
     2461 cccctcccca gcagaccaga gtgggtgaga aacccgccct gaacgacggc agcaagagat
     2521 acttcatcca catcaagaag attttccagg agagacactt ctacccaact ttcttgtaca
     2581 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     2641 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     2701 tggtcatagc tgtttcttgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     2761 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     2821 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     2881 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     2941 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     3001 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     3061 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     3121 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     3181 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     3241 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     3301 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     3361 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     3421 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     3481 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     3541 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     3601 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     3661 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     3721 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3781 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3841 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3901 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3961 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4021 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4081 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4141 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4201 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4261 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4321 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4381 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4441 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4501 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
