LOCUS       sequence-468003-       10095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express N-terminally FLAG tagged human Bag6 without UBL domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: PRK5-FLAG-Bag6-Delta-UBL
FEATURES             Location/Qualifiers
     source          1..10095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      918..927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             927..950
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4098..4116)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4136..4153)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4142..4366
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4412..4840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4854..5183
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5034..5169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5250..6044
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5304..5323)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5914..5933
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6218..6339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      6395..6404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6408..6542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6611..6968
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6611..6802
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      6819..6954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6988..7005)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6988..7004)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6997..7019)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7217..7672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7689..7708)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7808..7830
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7868..7886)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7954..8058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8059..8127,8128..8919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8277..8296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9090..9678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9579..9598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9832..9849
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9966..9987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10002..10007,10008..10025,10026..10032)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10040..10056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10045..10067
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10064..10080
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10064..10080
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc taagcttgcc gccatggatt acaaggatga cgacgataag gctcctcctc
      961 agactcacct cccttctggg gcatcttctg ggacggggtc tgcctcagcc actcatggtg
     1021 ggggatcccc ccctggtact cgggggcctg gggcctctgt tcatgaccgg aatgccaaca
     1081 gctatgtcat ggttggaacc ttcaatcttc ctagtgacgg ctctgctgtg gatgttcaca
     1141 tcaacatgga acaggccccg attcagagtg agccccgggt acggctggtg atggctcagc
     1201 acatgatcag ggatatacag accttactat cccggatgga gtgtcgagga gggccccaac
     1261 cgcagcacag tcagccgccc ccgcagccac cggctgtgac cccggagcca gtagccttga
     1321 gctctcaaac atcagaacca gttgaaagtg aagcacctcc ccgggagccc atggaggcag
     1381 aagaagtgga ggagcgtgcc ccagcccaga acccggagct cactcctggc ccagccccag
     1441 cgggcccaac acctgccccg gaaacaaatg cacccaacca tccttcccct gcggagtatg
     1501 tcgaggtgct ccaggagcta cagcggctgg agagtcgcct ccagcccttc ttgcagcgct
     1561 actacgaggt tctgggtgct gctgccacca cggactacaa taacaatcac gagggccggg
     1621 aggaggatca gcggttgatc aacttggtag gggagagcct gcgactgctg ggcaacacct
     1681 ttgttgcact gtctgacctg cgctgcaatc tggcctgcac gcccccacga cacctgcatg
     1741 tggtccggcc tatgtctcac tacaccaccc ccatggtgct ccagcaggca gccattccca
     1801 tacagatcaa tgtgggaacc actgtgacca tgacaggaaa tgggactcgg ccccccccaa
     1861 cccccaatgc agaggcacct ccccctggtc ctgggcaggc ctcatccgtg gctccgtctt
     1921 ctaccaatgt cgagtcctca gctgaggggg ctcccccgcc aggtccagct cccccgccag
     1981 ccaccagcca cccgagggtc atccggattt cccaccagag tgtggaaccc gtggtcatga
     2041 tgcacatgaa cattcaagat tctggcacac agcctggtgg tgttccgagt gctcccactg
     2101 gccccctggg accccctggt catggccaaa ccctgggaca gcaggtgcca ggcttcccaa
     2161 cagctccaac ccgggtggtg attgcccggc ccactcctcc acaggctcgg ccttcccatc
     2221 ctggagggcc cccagtctct gggacactgc agggcgccgg tctgggtacc aatgcctcgt
     2281 tggcccagat ggtgagcggc cttgtggggc agcttcttat gcagccagtc cttgtggctc
     2341 aggggacccc aggcatggct ccaccgccag cccctgccac tgcttctgcc agtgctggca
     2401 ccaccaacac agctaccaca gctggccccg ctcctggggg gcctgcccag cctccaccca
     2461 cccctcaacc ctccatggct gatcttcagt tctctcagct tctggggaac ctgctagggc
     2521 ctgcagggcc aggggctgga gggtctggtg tggcttctcc caccatcact gtggcgatgc
     2581 ctggtgtccc tgcctttctc caaggcatga ctgacttctt gcaggcaaca cagacagccc
     2641 ctccaccacc cccacctcct ccacccccac cacctgcccc agagcagcag accatgcccc
     2701 caccaggctc cccttctggt ggcgcaggga gtcctggagg cctgggtctt gagagcctgt
     2761 caccggagtt ttttacctca gtggtgcagg gtgtgctcag ctccctgctg ggctccctgg
     2821 gggctcgggc tggcagcagt gaaaatattg ctgccttcat acaacgcctc agtggatcca
     2881 gcaacatctt tgagcctgga gctgatgggg cccttggatt ctttggggcc ttgctttctc
     2941 ttctgtgcca gaacttctct atggtggacg tagtgatgct tctccatggg catttccagc
     3001 cactacaacg gctccagccc cagctgcgat ccttcttcca ccagcactac ctgggtggtc
     3061 aggagcccac acccagtaac atccggatgg caacccacac attgatcacg gggctagaag
     3121 agtatgtgcg ggagagtttt tccttggtgc aggttcagcc aggtgtggac atcatccgga
     3181 caaacctgga atttctccaa gagcagttta atagcattgc tgcgcatgtg ctgcattgca
     3241 cagatagtgg atttggggcc cggttgctgg agttgtgtaa ccaaggcctg tttgaatgcc
     3301 tggccctaaa cctgcactgc ttggggggac agcagatgga gcttgctgct gttatcaatg
     3361 gccgaattcg tcgtatgtct cgtggggtga atccctcctt ggtgagctgg ctgaccacta
     3421 tgatgggact gaggcttcag gtggtactgg agcacatgcc tgtaggccct gatgccattc
     3481 tcagatacgt tcgcagggtt ggtgatcccc cccagccact tcctgaggag ccaatggaag
     3541 ttcagggagc agaaagagct tcccctgagc ctcagcggga gaatgcttcc ccagcccctg
     3601 gaacaacagc agaagaggcc atgtcccgag gtccacctcc tgctcctgag gggggctccc
     3661 gggatgaaca ggatggagct tcagctgaga cagaaccttg ggcagctgca gtccccccag
     3721 aatgggtccc tattatccag caggacattc agagccagcg gaaggtgaaa ccgcagcccc
     3781 ctctgagtga tgcctacctc agtggtatgc ctgccaagag acgcaagacg atgcagggtg
     3841 agggccccca gctgcttctc tcagaggctg tgagccgggc agctaaggca gccggagctc
     3901 ggcccctgac gagccccgag agcctgagcc gggacctgga ggcaccagag gttcaggaga
     3961 gctacaggca gcagctccgg tctgatatac aaaaacgact gcaggaagac cccaactaca
     4021 gtccccagcg cttccccaat gcccagcggg cctttgctga tgatccttag ctcgagcatg
     4081 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     4141 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4201 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4261 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4321 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4381 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4441 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4501 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4561 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4621 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4681 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4741 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4801 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4861 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4921 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4981 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5041 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5101 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5161 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5221 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5281 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5341 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5401 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5461 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5521 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5581 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5641 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5701 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5761 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     5821 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5881 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5941 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6001 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6061 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     6121 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6181 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6241 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6301 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgag
     6361 gatccccggg taccgagctc gaattcaatc gatggccgcc atggcccaac ttgtttattg
     6421 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6481 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     6541 tcgggaatta attcggcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     6601 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     6661 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6721 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6781 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6841 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6901 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6961 tttgcaaaaa gctgttaaca gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     7021 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     7081 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     7141 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     7201 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7261 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7321 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7381 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     7441 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7501 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     7561 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7621 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     7681 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     7741 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     7801 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     7861 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     7921 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7981 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8041 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     8101 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     8161 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     8221 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8281 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     8341 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8401 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     8461 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     8521 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     8581 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     8641 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8701 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8761 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8821 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     8881 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8941 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9001 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9061 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9121 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9181 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     9241 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9301 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9361 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9421 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9481 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9541 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9601 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9661 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9721 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9781 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     9841 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     9901 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9961 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    10021 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    10081 atgattacga attaa
//
