LOCUS       sequence-468006-        5478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF574 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF574
FEATURES             Location/Qualifiers
     source          1..5478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2789..2888)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2905..2924)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2906..2924)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2929..2945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2929..2945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3376..4167
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4260..4848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4749..4768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5002..5019
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5178..5205)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5297..5383)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5432..5454
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5446..5463
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5447..5463
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgactgagga atcagaggag
      121 acagtcctgt acattgagca ccgctatgtc tgctctgagt gcaaccagct gtatggatca
      181 ctggaagagg tgcttatgca ccaaaactcc cacgtgcccc agcagcactt tgagctggtg
      241 ggcgtggctg atcccggagt cactgtggcc acagacacag cttcaggcac gggcctctat
      301 cagacccttg tgcaggagag ccagtaccag tgcctggagt gtggtcaact gctgatgtca
      361 cccagccagc tcctggagca ccaggagctg cacctgaaga tgatggcacc ccaggaggca
      421 gtgccagctg agccatcacc taaggcacca cccctgagct ccagcaccat ccactacgag
      481 tgtgtggatt gcaaggctct ctttgccagc caggagctct ggctgaacca ccggcagacg
      541 cacctccggg ccacacccac caaggctcct gcccctgttg tcctggggtc cccagttgtt
      601 ctagggcctc ctgtgggcca ggcccgagtg gctgtggagc actcataccg aaaggcagaa
      661 gagggtgggg aaggggcgac tgtcccatct gccgctgcca ccaccactga ggtagtgact
      721 gaggtggagc tgctcctcta caagtgctct gagtgctccc agctcttcca gctgccggcg
      781 gatttcctgg agcaccaggc cactcacttc cctgctcctg tacccgagtc tcaggagcct
      841 gccttacagc aggaggtgca ggcctcgtca cctgcagagg tgcctgtgtc tcagcctgac
      901 cccttgccag cttctgacca cagttacgag ctgcgcaatg gtgaagccat tgggcgggat
      961 cgccgggggc gcagggcccg gaggaacaac agtggagaag caggcggggc agccacacag
     1021 gagctcttct gctcagcctg tgaccagctc tttctctcac cccaccagct acagcagcac
     1081 ctgcggagtc accgggaggg cgtctttaag tgccccctgt gcagtcgtgt cttccctagc
     1141 ccttccagtc tggaccagca ccttggagac catagcagcg agtcacactt cctgtgtgta
     1201 gactgtggcc tggccttcgg cacagaggcc ctcctcctgg cccaccggcg agcccacacc
     1261 ccgaatcctc tgcattcatg tccatgtggg aagacctttg tcaaccttac caagttcctt
     1321 tatcaccggc gtactcatgg ggtagggggt gtccctctgc ccacaacacc agtcccacca
     1381 gaggaacctg tcattggttt ccctgagcca gccccagcag agactggaga gccagaggcc
     1441 cctgagcccc ctgtgtctga ggagacctca gcagggcccg ctgccccagg cacctaccgc
     1501 tgcctcctgt gcagccgtga atttggaaag gccttgcagc tgacccggca ccaacgtttt
     1561 gtgcatcggc tggagcggcg ccataaatgc agcatttgtg gcaagatgtt caagaagaag
     1621 tctcacgtgc gtaaccacct gcgcacacac acaggggagc ggcccttccc ctgccctgac
     1681 tgctccaagc ccttcaactc acctgccaac ctggcccgcc accggctcac acacacagga
     1741 gagcggccct accggtgtgg ggactgtggc aaggctttca cgcaaagctc cacactgagg
     1801 cagcaccgct tggtgcatgc ccagcacttc ccctaccgct gccaggaatg tggggtgcgt
     1861 tttcaccgtc cttaccgcct gctcatgcac cgctaccatc acacaggtga atacccctac
     1921 aagtgtcgcg agtgcccccg ctccttcttg ctgcgtcggc tgctggaggt gcaccagctc
     1981 gtggtccatg ccgggcgcca gccccaccgc tgcccatcct gtggggctgc cttcccctcc
     2041 tcactgcggc tccgggagca ccgctgtgca gccgctgctg cccaggcccc acggcgcttt
     2101 gagtgtggca cctgtggcaa gaaagtgggc tcagctgctc gactgcaggc acacgaggcg
     2161 gcccatgcag ctgctgggcc tggagaggtc ctggctaagg agccccctgc ccctcgagcc
     2221 ccacgggcca ctcgtgcacc agttgcctct ccagcagccc ttggaagcac tgctacagca
     2281 tcccctgcgg cccctgcccg ccgccggggt ctagagtgca gcgagtgcaa gaagctgttc
     2341 agcacagaga cgtcactgca ggtgcaccgg cgcatccaca caggtgagcg gccataccca
     2401 tgtccagact gtggcaaagc gttccgtcag agtacccacc tgaaagacca ccggcgcctg
     2461 cacacaggtg agcggccctt tgcctgtgaa gtgtgtggca aggcctttgc catctccatg
     2521 cgcctggcag aacatcgccg catccacaca ggcgaacgac cctactcctg ccctgactgt
     2581 ggcaagagct accgctcctt ctccaacctc tggaagcacc gcaagaccca tcagcagcag
     2641 catcaggcag ctgtgcggca gcagctggca gaggcggagg ctgccgttgg cctggccgtc
     2701 atggagactg ctgtggaggc gctacccctg gtggaagcca ttgagatcta ccctctggcc
     2761 gaggctgagg gggtccagat cagtggctgc ccaactttct tgtacaaagt tggcattata
     2821 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     2881 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     2941 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     3001 atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     3061 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     3121 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     3181 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     3241 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     3301 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     3361 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     3421 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     3481 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     3541 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     3601 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     3661 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     3721 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     3781 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     3841 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     3901 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     3961 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     4021 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     4081 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     4141 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     4201 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4381 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4621 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4861 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4921 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4981 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5041 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5101 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     5161 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     5221 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     5281 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     5341 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     5401 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     5461 agtcttaagc tcgggccc
//
