LOCUS       sequence-468032-        9033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid 
            encoding the two fragments .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Garcia Casas P, Rossini M, Pavenius L, Saeed M, Arnst N, Sonda S, 
            Fernandes T, D'Arsie I, Bruzzone M, Berno V, Raimondi A, Sassano ML,
            Naia L, Barbieri E, Sigismund S, Agostinis P, Sturlese M, Niemeyer 
            BA, Brismar H, Ankarcrona M, Gautier A, Pizzo P, Filadi R
  TITLE     Simultaneous detection of membrane contact dynamics and associated 
            Ca(2+) signals by reversible chemogenetic reporters.
  JOURNAL   Nat Commun. 2024 Nov 12;15(1):9775. doi: 10.1038/s41467-024-52985-0.
  PUBMED    39532847
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-11-12"; volume: "15(1):9775. doi"; pages: " 
            10.1038/s41467-024-52985-0"
COMMENT     Sequence Label: ER-mit RspA-splitFAST (lentiviral plasmid)
FEATURES             Location/Qualifiers
     source          1..9033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     361..381
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        363..381
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        409..635
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             636..816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    863..988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1481..1714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1899..1943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2335..2355
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2416..2719
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2720..2923
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2874..2894
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2944..2968
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2975..2984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3477..3501)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3505..3525)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3510..3839
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3690..3825
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      3851..3860
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4121..4141)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    4154..4742
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4207..4227)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4625..4636)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4773..5272
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4808..4831)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5205..5224
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5289..5888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5289..5308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5785..5805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5960..6193
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6265..6386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6302..6321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6356..6375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6426..6561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6581..6600)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6582..6600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6610..6627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6610..6626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6619..6641)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6768..7223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6855..6874)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7065..7086
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7249..7353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7354..7422,7423..8214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7572..7591)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8385..8973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8874..8893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgctagctt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttacta gtatcaactt
     2341 tgtatagaaa agttgtagtt attaatagta atcaattacg gggtcattag ttcatagccc
     2401 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     2461 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2521 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2581 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2641 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2701 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2761 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2821 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2881 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
     2941 gatccaagtt tgtacaaaaa agcaggctgg taccgccacc atggaccctg tggtggtgct
     3001 ggggctctgt ctctcctgtt tgcttctcct ttcactctgg aaacagagct atgggggagg
     3061 gtctggtgga tctggaggaa ccgatatcgt ctccggagga ggcggcagcg gcggaggggg
     3121 atcaatggag accgtgagat tcggcggcga cgacatcgag aacagcctgg ccaagatgga
     3181 cgacaaggcc ctggacaagc tggccttcgg cgccatccag ctggacggca acggcaagat
     3241 catccactac aacgccgccg agggcaccat caccggcaga gaccccaaga ccgtgatcgg
     3301 caagaacttc ttcaccgacg tggcccccgg cacccagagc aaggagttcc agggcagatt
     3361 caaggagggc gtgcagaagg gcgacctgaa caccatgttc gagtggatga tccccaccag
     3421 cagaggcccc accaaggtga aggtgcacat gaagaaggcc atgacctaac tcgagtaccc
     3481 agctttcttg tacaaagtgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3541 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3601 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3661 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3721 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     3781 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3841 agcttctaga gccaccatgg ccgcctctaa acctgtggag gcagcggtgg tcgcagccgc
     3901 tgtaccgagc tccgggagtg gggtgggcgg cggcgggact gcgggcccgg gcacgggggg
     3961 gctgccgcga tggcagctgg ctctggcggt cggggcaccc ctgctgctgg gcgcgggtgc
     4021 catatacctg tggagtcggc agcaacggcg ccgggaggcc agaggccggg gcggatccgg
     4081 cgacagcttc tggatcttcg tgaagagact gtaactgcag caactttatt atacatagtt
     4141 gatcaattcc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4201 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4261 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4321 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4381 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4441 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4501 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     4561 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4621 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4681 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4741 gcatcgggaa ttcccgcggt tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca
     4801 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     4861 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     4921 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     4981 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     5041 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     5101 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     5161 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     5221 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctcacg
     5281 tggccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     5341 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5401 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5461 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5521 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5581 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5641 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5701 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5761 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5821 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5881 gtgcctgagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5941 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     6001 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6061 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6121 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6181 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     6241 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     6301 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6361 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6421 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6481 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6541 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6601 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6661 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6721 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6841 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6901 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6961 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7021 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7081 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7141 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     7201 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     7261 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7321 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7381 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7441 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7501 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7561 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7621 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7681 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7741 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7801 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7861 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7921 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7981 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8041 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8101 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8161 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8221 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8281 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8341 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8401 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8461 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8521 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8581 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8641 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8701 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8761 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagagagaaa ggcggacagg
     8821 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8881 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8941 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9001 ttcctggcct tttgctggcc ttttgctcac atg
//
