LOCUS       sequence-468045-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses photoactivable fluorescent reporter for visualizing 
            mitochondrial network.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Mondal, P
  TITLE     Photoactivable plasmid labeling Mitochondria for Mammalian 
            expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Mito-Dendra2
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             100..789
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
       61 gtgccgcgcg ccaagatcca ttcgttgggg gatccaacca tgaacacccc gggaattaac
      121 ctgatcaagg aggacatgcg cgtgaaggtg cacatggagg gcaacgtgaa cggccacgcc
      181 ttcgtgatcg agggcgaggg caagggcaag ccctacgagg gcacccagac cgccaacctg
      241 accgtgaagg agggcgcccc cctgcccttc agctacgaca tcctgaccac cgccgtgcac
      301 tacggcaacc gggtgttcac caagtacccc gaggacatcc ccgactactt caagcagagc
      361 ttccccgagg gctacagctg ggagcgcacc atgaccttcg aggacaaggg catctgcacc
      421 atccgcagcg acatcagcct ggagggcgac tgcttcttcc agaacgtgcg cttcaagggc
      481 accaacttcc cccccaacgg ccccgtgatg cagaagaaga ccctgaagtg ggagcccagc
      541 accgagaagc tgcacgtgcg cgacggcctg ctggtgggca acatcaacat ggccctgctg
      601 ctggagggcg gcggccacta cctgtgcgac ttcaagacca cctacaaggc caagaaggtg
      661 gtgcagctgc ccgacgccca cttcgtggac caccgcatcg agatcctggg caacgacagc
      721 gactacaaca aggtgaagct gtacgagcac gccgtggccc gctacagccc cctgcccagc
      781 caggtgtggt ccggcgacag cggcgtgtac aagactcgag ttcgtgaccg ccgccgccat
      841 cacccacggc atggatgagc tgtacaagta a
//
