LOCUS       sequence-468047-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses photoactivable fluorescent reporter for visualizing 
            mitochondrial network.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Aravinth S, Varghese JM, Pant N, Mondal PP
  TITLE     Super-resolution optical microscopy reveals accumulation of 
            photoactivable dengue protein (Dendra2-NS2B) in the endoplasmic 
            reticulum.
  JOURNAL   Sci Rep. 2025 Mar 26;15(1):10358. doi: 10.1038/s41598-025-94135-6.
  PUBMED    40133359
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2025-03-26"; volume: "15(1):10358. doi"; pages: " 
            10.1038/s41598-025-94135-6"
COMMENT     Sequence Label: Mito-PAmKate
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..143
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             168..881
                     /codon_start=1
                     /product="monomeric photoactivatable red fluorescent 
                     protein (Gunewardene et al., 2011)"
                     /label=PAmKate
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRGD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gggctgtttt aggcaacgtg gggagttata tagcagagct ggtttagtga accgtcagat
       61 ccgctagcat gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc tcggcccggc
      121 ggctcccagt gccgcgcgcc aagatccatt cgttggggga tccaaccatg gtgtctaagg
      181 gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
      241 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
      301 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
      361 gcttcatgta cggcagcaag accttcatca accacaccca gggcatcccc gacttctgga
      421 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
      481 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
      541 tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
      601 aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga ggggacatgg
      661 ccctgaagct cgtgggcggg ggccacctga tctgcaactt gaagaccaca tacagatcca
      721 agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccgc agactggaaa
      781 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat
      841 actgcgacct ccctagcaaa ctggggcaca agcttaatta actcgagttc gtgaccgccg
      901 ccgccatcac ccacggcatg gatgagctgt acaagtaa
//
