LOCUS       sequence-468102-       10633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for 
            visualization of the Mut mRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Zhang Z, Xu A, Bai Y, Chen Y, Cates K, Kerr C, Bermudez A, Susanto 
            TT, Wysong K, Garcia Marques FJ, Nolan GP, Pitteri S, Barna M
  TITLE     A subcellular map of translational machinery composition and 
            regulation at the single-molecule level.
  JOURNAL   Science. 2025 Mar 7;387(6738):eadn2623. doi: 
            10.1126/science.adn2623. Epub 2025 Mar 7.
  PUBMED    40048539
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-03-7"; volume: "387(6738):eadn2623. doi"; pages: " 
            10.1126/science.adn2623. Epub 2025 Mar 7"
COMMENT     Sequence Label: pcDNA4TO_24xGCN4_MUT_24xPP7
FEATURES             Location/Qualifiers
     source          1..10633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1059..1115
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1131..1187
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1203..1259
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1275..1331
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1347..1403
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1419..1475
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1491..1547
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1563..1619
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1635..1691
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1707..1763
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1779..1835
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1851..1907
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1923..1979
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1995..2051
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2067..2123
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2139..2195
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2211..2267
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2283..2339
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2355..2411
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2427..2483
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2499..2555
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2571..2627
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2715..2771
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(6641..6658)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6647..6871
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6917..7345
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7004..7023)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7214..7235
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7354..7374)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7359..7688
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7539..7674
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7601..7620
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7736..7783
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7802..8176
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8306..8427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8343..8362)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8397..8416
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8476..8492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8476..8492)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8489..8511)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8500..8516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8524..8530,8531..8548,8549..8554))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8569..8590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8707..8724)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8878..9466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8958..8977)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9637..10428,10429..10497))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10260..10279
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10498..10602)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
     1021 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt
     1081 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc
     1141 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg
     1201 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag
     1261 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc
     1321 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa
     1381 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt
     1441 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc
     1501 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg
     1561 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag
     1621 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga
     1681 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga
     1741 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact
     1801 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat
     1861 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg
     1921 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg
     1981 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga
     2041 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga
     2101 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact
     2161 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat
     2221 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg
     2281 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag
     2341 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc
     2401 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga
     2461 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact
     2521 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac
     2581 tcagtaagaa ttaccacctg gaaaacgaag ttgcacgttt gaagaaagga tcaggatcag
     2641 gcgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg
     2701 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc
     2761 gacttaaaaa gggttctggc tcgggtcagc ggccgcagtt gagagctaag aatcaacttt
     2821 ttttgctatc gccccattac ctgaagcagc taaacattcc atcagcttcc agatggaaac
     2881 gccttctaca ccagcagcaa ccccttcacc cagaatgggc tgtactggcc aaaaagcagc
     2941 tgaaaggcaa aaacccagag gaccttatat ggcacacccc agaagggatc tctataaagc
     3001 ccttatattc cagggcagat actctggact tacccgaaga acttccagga gtgaagccat
     3061 tcacacgggg accatatcct accatgtata cctataggcc ctggaccatc cgtcagtatg
     3121 caggctttag tactgtggaa gaaagcaata aattctataa ggacaatatt aaggctggtc
     3181 agcaggggtt gtctgttgcc tttgacttgg caacacatcg tggttatgat tcagacaacc
     3241 ccagagttcg tggagatgtt ggaatggctg gagttgctat tgacactgta gaagacacca
     3301 aaatcctatt tgatggcatt cctttagaaa aaatgtccgt ttccatgact atgaacggag
     3361 ctgtcatccc agtcctggca acatttatag taactgggga agagcaaggt gtgccgaagg
     3421 agaagctcac tggcacaatt cagaatgaca tcctaaagga gtttatggtc agaaatactt
     3481 atatttttcc cccagagcca tcgatgaaaa ttattgctga cattttccaa tacacagcac
     3541 agcacatgcc aaaatttaat tccatttcga ttagcgggta ccatatgcag gaagcaggag
     3601 ctgatgccat tttagaactg gcctatacca tcgcagatgg gttggagtac tgcagaactg
     3661 gactccaggc tggactcaca attgatgaat ttgcaccaag gttgtctttc ttctggggaa
     3721 ttggaatgaa cttctacatg gaaatagcca agatgcgagc tgggagaaga ctgtgggctc
     3781 acttaataga aaaaatgttc cagcctaaaa actctaaatc tcttcttcta agagcacact
     3841 gccagacatc ggggtggtca cttactgagc aggatcctta caataacatt gtccgcactg
     3901 caatcgaagc catggcagct gtgtttggag gaacccagtc tttgcatacg aactcttttg
     3961 atgaagcgct gggtttgccc actgtgaaaa gtgcccggat tgctcggaac acacagatca
     4021 tcattcagga agaatctggg atccccaaag tggcggatcc ttggggaggg tcgtacatga
     4081 tggagtcgct cacaaatgac gtttatgagg ctgctctgaa gttgatatat gaagttgaag
     4141 aaatgggtgg aatggccaaa gctgtagctg aaggaatccc taaacttcgc attgaagaat
     4201 gtgctgcccg aagacaagct agaatagatt ctggttctga ggtaattgtt ggagtaaata
     4261 agtatcagtt ggaaaaagaa gactctgtgg aggtcctggc cattgacaac acttcagtgc
     4321 gtaagaagca gattgaaaaa ctcaagaaga ttaaatccag cagggatcaa gctttggctg
     4381 agcagtgtct cagtgcactt acccagtgtg ctgccagtgg agatggcaat attctggctc
     4441 tggcagtgga tgcagctcgt gcaagatgta cagttggaga aatcacggat gccttgaaaa
     4501 aagtatttgg tgagcataaa gctaatgatc gtatggtgag tggagcatat cggcaggagt
     4561 ttggagaaag taaagagatc acatctgcca tcaagagagt taataaattc atggaacgtg
     4621 aaggtcgcag acctcgtctt cttgtggcaa aaatgggaca agatggccat gacaggggag
     4681 ccaaggtcat tgctacagga tttgctgatc ttggttttga tgtggacata ggccctcttt
     4741 ttcagactcc ccgtgaagtg gcgcagcaag ctgtggatgc agatgtccat gctgtgggtg
     4801 tcagcacact tgctgctggt cataaaaccc tcgttcctga gcttatcaaa gaactcaccg
     4861 ccctggggcg gccagatatc cttgtcatgt gtgggggcgt gattccacca caggattatg
     4921 aatttctgta tgaagttggt gtttccaacg tctttggtcc tggaacccgg attccaagag
     4981 ctgctgtcca agtgcttgat gatattgaga agtgtttggc agagaagcag cagtctgtga
     5041 tctaacgaca cgattcgaag atcgcatcgc tagcccggtt gtacaggtac cggatcctaa
     5101 ggtacctaat tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct
     5161 agaaaccagc agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta
     5221 aggtacctaa ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc
     5281 tagaaaccag cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatcct
     5341 aaggtaccta attgcctaga aaggagcaga cgatatggcg tcgctccctg caggtcgact
     5401 ctagaaacca gcagagcata tgggctcgct ggctgcagta ttcccgggtt cattagatcc
     5461 taaggtacct aattgcctag aaaggagcag acgatatggc gtcgctccct gcaggtcgac
     5521 tctagaaacc agcagagcat atgggctcgc tggctgcagt attcccgggt tcattagatc
     5581 ctaaggtacc taattgccta gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga
     5641 ctctagaaac cagcagagca tatgggctcg ctggctgcag tattcccggg ttcattagat
     5701 cctaaggtac ctaattgcct agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg
     5761 actctagaaa ccagcagagc atatgggctc gctggctgca gtattcccgg gttcattaga
     5821 tcctaaggta cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc
     5881 gactctagaa accagcagag catatgggct cgctggctgc agtattcccg ggttcattag
     5941 atcctaaggt acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt
     6001 cgactctaga aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta
     6061 gatcctaagg tacctaattg cctagaaagg agcagacgat atggcgtcgc tccctgcagg
     6121 tcgactctag aaaccagcag agcatatggg ctcgctggct gcagtattcc cgggttcatt
     6181 agatcctaag gtacctaatt gcctagaaag gagcagacga tatggcgtcg ctccctgcag
     6241 gtcgactcta gaaaccagca gagcatatgg gctcgctggc tgcagtattc ccgggttcat
     6301 tagatcctaa ggtacctaat tgcctagaaa ggagcagacg atatggcgtc gctccctgca
     6361 ggtcgactct agaaaccagc agagcatatg ggctcgctgg ctgcagtatt cccgggttca
     6421 ttagatccta aggtacctaa ttgcctagaa aggagcagac gatatggcgt cgctccctgc
     6481 aggtcgactc tagaaaccag cagagcatat gggctcgctg gctgcagtat tcccgggttc
     6541 attagatctc gcgaagggcg aattcacgag gcgcgcccgg atcgcgatcg caatcttaat
     6601 taactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     6661 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     6721 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6781 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6841 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     6901 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6961 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7021 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7081 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7141 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7201 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7261 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7321 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     7381 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7441 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7501 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7561 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7621 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7681 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     7741 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     7801 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     7861 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     7921 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     7981 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     8041 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     8101 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     8161 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     8221 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8281 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8341 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8401 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8461 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8521 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8581 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8641 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8701 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8761 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8821 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8881 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8941 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9001 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9061 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9121 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9181 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9241 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9301 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9361 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9421 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9481 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9541 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9601 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9661 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9721 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9781 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9841 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9901 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9961 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10021 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10081 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10141 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10201 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10261 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10321 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10381 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10441 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10501 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10561 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10621 gccacctgac gtc
//
