LOCUS       sequence-468107-        8827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, 
            Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W
  TITLE     Visualization of retroviral replication in living cells reveals 
            budding into multivesicular bodies.
  JOURNAL   Traffic 2003 Nov;4(11):785-801.
  PUBMED    14617360
REFERENCE   2  (bases 1 to 8827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic"; 
            date: "2003-11"; volume: "4(11)"; pages: "785-801"
COMMENT     Sequence Label: Friend MLV Env YFP
FEATURES             Location/Qualifiers
     source          1..8827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        133..512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        513..716
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     667..687
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     761..780
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        761..779
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2400..3110
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(2439..2460)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2700..2719)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3047..3068
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4278..4296)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4279..4296)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4316..4333)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4322..4546
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4592..5020
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4679..4698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4889..4910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5029..5049)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5034..5363
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5214..5349
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5276..5295
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5430..6224
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5484..5503)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6094..6113
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6398..6519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6435..6454)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6489..6508
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6568..6584)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6568..6584)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6581..6603)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6592..6608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6616..6622,6623..6640,6641..6646))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6661..6682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6799..6816)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6970..7558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7050..7069)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7729..8520,8521..8589))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8352..8371
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8590..8694)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8769..8788)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
       61 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
      121 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
      721 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
      781 agacccaagc tttcccaacc aagaaagaaa ctgccaaagt tgtaaccaag aagctactaa
      841 aagaaatctt ccccagattc ggcatgccac aggtattggg aaccaacaat gggcctgccc
      901 tcgtctccaa ggtaagtcag acagtagccg atttattggg ggttgattgg aaactacatt
      961 gtgcttacag accccagagt tcaggtcagg tagaaagaat gaataggaca atcaaggaga
     1021 ctttaactaa attgacgctt gcaactggct ctagggactg ggtgctcctg cttcccccgg
     1081 ccctgtatca agcccgcaac acgccgggcc cccatggtct caccccatat gacatggcaa
     1141 aggtcactca taacccctct ctccaagctc atttacaggc actctacttg gtccagcaca
     1201 aagtctggag accgttggcg gcagcttacc aagaacaact agaccggccg gtagtacctc
     1261 accctttccg agtcggtgac acagtgtggg tccgcagaca ccagcggcat cgctgcgtgg
     1321 atcccctcga ctctagaacc ccgctggaaa ggaccctata ccgtcctact gactaccccc
     1381 accgctctca aagtagacgg cattgcagcg tggatccacg ctgcccacgt aaaggctgcc
     1441 gacaccagga ttgagccacc agcagaatcg acatggcgtg ttcaacgctc ccaaaatccc
     1501 ctaaagataa gattgacccg cgggacctcc taatcccctt aattctcttc ctgtctctca
     1561 aaggggccag atccgcagca cccggctcca gccctcacca ggtctacaac attacctggg
     1621 aagtgaccaa tggggatcgg gagacagtat gggcaatatc aggcaaccac cctctgtgga
     1681 cttggtggcc agtcctcacc ccagatttgt gtatgttagc tctcagtggg ccgccccact
     1741 gggggctaga gtatcaggcc ccctattcct cgcccccggg gcccccttgt tgctcaggga
     1801 gcagcgggag cagtgcaggc tgttccagag actgcgacga gcccttgacc tccctcaccc
     1861 ctcggtgcaa cactgcctgg aacagactta agctagatca ggtaactcat aaatcaagtg
     1921 agggatttta tgtctgcccc gggtcacatc gcccccggga agccaagtcc tgtggaggtc
     1981 cagactcctt ctactgtgcc tcttggggct gcgagacaac cggtagagta tactggaagc
     2041 cctcctcctc ttgggactac atcacagtgg acaacaatct caccactagc caggctgtcc
     2101 aggtatgcaa agacaataag tggtgcaatc ccttggctat ccagtttaca aacgccggga
     2161 aacaggtcac ctcatggaca actggacact attggggtct acgtctttat gtctctgggc
     2221 gggacccggg gcttactttc gggatccgac tcagatatca aaatctagga cctcgggtcc
     2281 cgataggacc gaaccccgtc ctggcagacc aactttcgct cccgcgacct aatcccctac
     2341 ccaaacctgc caagtctccc cccgcctcta attcgactcc cacattgatt tccgctagca
     2401 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2461 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     2521 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     2581 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     2641 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2701 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2761 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2821 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     2881 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     2941 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3001 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3061 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gctagcgctg
     3121 ttgccggcgc cccgtccccc actcccactc agcccccgcc agcaggaacg ggagacaggt
     3181 tactaaatct agtacaggga gcttaccagg cactcaacct taccaaccct gataaaactc
     3241 aagagtgctg gttatgccta gtgtctggac ccccctatta cgaaggggtt gccgtcctag
     3301 gtacttattc caaccatacc tctgccccag ctaactgctc cgtggcctcc caacacaagt
     3361 tgaccctgtc cgaagtgact ggacggggac tctgcatagg aacagtccca aaaactcacc
     3421 aggccctgtg caacactacc cttaagatag acaaagggtc ttactatcta gttgccccca
     3481 caggaactac gtgggcatgt aacactggac tcactccatg cctatctgcc accgtgctta
     3541 atcgcaccac tgactattgc gttctcgtag agttatggcc cagggtcacc taccatcctc
     3601 ccagttacgt ctatagccag tttgaaaaat cctatagaca taaaagagaa ccagtgtcct
     3661 taaccttggc cctattatta ggtgggctaa ctatgggtgg catcgccgcg ggagtaggga
     3721 caggaactac cgccctggtc gccacccagc agttccagca gctccatgct gccgtacaag
     3781 atgatctcaa agaagtcgaa aagtcaatta ctaacctaga aaagtctctt acttcgttgt
     3841 ctgaggttgt gctgcagaat cgacgaggcc tagacctgtt gttcctaaaa gaaggaggac
     3901 tgtgtgctgc cctaaaagaa gaatgttgtt tctatgctga ccatacaggc ctagtaagag
     3961 atagtatggc caaattaaga gagagactca ctcagagaca aaaactattt gagtcgagcc
     4021 aaggatggtt cgaaggattg tttaacagat ccccctggtt taccacgtta atatccacca
     4081 tcatggggcc tctcattata ctcctactaa ttctgctttt tggaccctgc attcttaatc
     4141 gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac
     4201 aatatcacca gctgaagcca ctagaatacg agccatgata agaattctgc agatatccat
     4261 cacactggcg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     4321 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4381 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4441 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4501 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4561 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4621 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4681 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4741 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4801 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4861 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4921 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4981 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5041 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5101 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5161 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5221 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5281 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5341 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5401 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5461 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5521 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5581 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5641 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5701 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5761 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5821 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5881 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5941 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6001 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6061 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6121 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6181 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6241 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     6301 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6361 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6421 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6481 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6541 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6601 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6661 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6721 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6781 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6841 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6901 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6961 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7021 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7081 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7141 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7201 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7261 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7321 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7381 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7441 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7501 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7561 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7621 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7681 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7741 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7801 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7861 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7921 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7981 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8041 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8101 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8161 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8221 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8281 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8341 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8401 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8461 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8521 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8581 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8641 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8701 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     8761 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     8821 gtgttgg
//
