LOCUS       sequence-468114-       10093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To create cASIC1 channels of defined stoichiometry in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10093)
  AUTHORS   Purohit R, Couch T, Rook ML, MacLean DM
  TITLE     Proline substitutions in the ASIC1 beta11-12 linker slow 
            desensitization.
  JOURNAL   Biophys J. 2024 Oct 15;123(20):3507-3518. doi: 
            10.1016/j.bpj.2024.08.016. Epub 2024 Sep 3.
  PUBMED    39182166
REFERENCE   2  (bases 1 to 10093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2024.08.016"; journalName: "Biophys J"; date: 
            "2024-10-15- 15"; volume: "123"; issue: "20"; pages: "3507-3518"
COMMENT     Sequence Label: cASIC1 concatemer - fully assembled
FEATURES             Location/Qualifiers
     source          1..10093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(260..364)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(439..458)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        630..1009
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1010..1213
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1164..1184
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1258..1277
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1258..1276
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6163..6180)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6169..6393
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6439..6867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6526..6545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6736..6757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6876..6896)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6881..7210
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7061..7196
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7123..7142
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7277..7987
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      7532..7541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8161..8282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8198..8217)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8252..8271
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8331..8347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8331..8347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8344..8366)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8355..8371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8379..8385,8386..8403,8404..8409))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8424..8445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8562..8579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8733..9321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8813..8832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9492..10093,1..190,191..259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
       61 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
      421 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
      481 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      541 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      601 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      661 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      721 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      781 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      841 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      901 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      961 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1021 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1081 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1141 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1201 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     1261 tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag
     1321 ctcggatcca ctagtccagg agccaccatg atggacctga aggtggacga ggaggaggtg
     1381 gacagcgggc agccggtgag catccaggcc ttcgccagca gctccaccct gcacgggatc
     1441 tcacacatct tctcctacga gcggctgtcg ctgaagcgcg tggtctgggc gctctgcttc
     1501 atgggttcgc tggcgctgct cgccctggtc tgcaccaacc gcatccagta ctacttcctc
     1561 tacccccacg tcaccaagct ggacgaggtg gccgccacca ggctcacctt ccctgctgtc
     1621 accttctgca acctcaacga gttccgcttc agccgggtga ccaagaacga cctgtaccac
     1681 gccggggaac tcctcgccct gctcaataac agatacgaga tcccggacac acagacagct
     1741 gatgagaagc agctggaaat cctacaggac aaggcaaact tccgaaactt caagcccaaa
     1801 cctttcaata tgttggagtt ttacgaccgc gccggccacg acatccggga gatgctgctc
     1861 tcctgcttct tccgtgggga gcagtgcagc cctgaggact tcaaagtggt gttcacacgc
     1921 tatgggaagt gctacacgtt caacgcaggg caggatggca aaccccggct catcaccatg
     1981 aaggggggca ctggaaatgg cctggagatc atgctggaca tccagcagga cgagtacctc
     2041 ccagtgtggg gggaaacaga tgagacctca tttgaagccg ggatcaaggt gcagatccac
     2101 agccaggatg agcctccact catcgaccag ctgggcttcg gtgtggcacc cggcttccag
     2161 accttcgtgt cctgccagga gcagcggctc atctacctac cacctccctg gggcgactgt
     2221 aaggccacaa cgggtgactc ggagttctac gacacttaca gcatcactgc ctgccgcatc
     2281 gactgcgaga cccgctacct ggtggagaac tgcaactgcc gcatggtgca catgccaggc
     2341 gatgcccctt attgcacccc agagcagtac aaggagtgtg cagatccagc cttagatttc
     2401 ctggtggaga aggacaatga gtactgtgtc tgtgagatgc cctgcaacgt cacccgctat
     2461 ggcaaagagc tctccatggt gaagatcccc agcaaagcct ccgccaagta cctggcaaag
     2521 aagtacaaca agtcggagca gtacatcggg gagaacatcc tggtgctgga catcttcttt
     2581 gaagccctga actatgagac aatcgagcag aagaaggcgt atgaggtggc tggattgctg
     2641 ggtgacatcg ggggacagat ggggctgttt atcggggcca gcatcctcac tgtgctggag
     2701 ctctttgact acgcctatga ggtgatcaag caccggctgt gccggcgggg caaatgccgc
     2761 aagaaccaca agaggaacaa cacggacaag ggcgtcgcgc tgagcatgga tgatgtgaaa
     2821 cgccacaacc cctgtgagag cctacggggt cacccggctg gcatgacgta cgcagcgaac
     2881 atcctacctc accacccggc ccggggcacc tttgaggact tcacctgcgc aggtaccgtg
     2941 aacagcatga tggacctgaa ggtggacgag gaggaggtgg acagcgggca gccggtgagc
     3001 atccaggcct tcgccagcag ctccaccctg cacgggatct cacacatctt ctcctacgag
     3061 cggctgtcgc tgaagcgcgt ggtctgggcg ctctgcttca tgggttcgct ggcgctgctc
     3121 gccctggtct gcaccaaccg catccagtac tacttcctct acccccacgt caccaagctg
     3181 gacgaggtgg ccgccaccag gctcaccttc cctgctgtca ccttctgcaa cctcaacgag
     3241 ttccgcttca gccgggtgac caagaacgac ctgtaccacg ccggggaact cctcgccctg
     3301 ctcaataaca gatacgagat cccggacaca cagacagctg atgagaagca gctggaaatc
     3361 ctacaggaca aggcaaactt ccgaaacttc aagcccaaac ctttcaatat gttggagttt
     3421 tacgaccgcg ccggccacga catccgggag atgctgctct cctgcttctt ccgtggggag
     3481 cagtgcagcc ctgaggactt caaagtggtg ttcacacgct atgggaagtg ctacacgttc
     3541 aacgcagggc aggatggcaa accccggctc atcaccatga aggggggcac tggaaatggc
     3601 ctggagatca tgctggacat ccagcaggac gagtacctcc cagtgtgggg ggaaacagat
     3661 gagacctcat ttgaagccgg gatcaaggtg cagatccaca gccaggatga gcctccactc
     3721 atcgaccagc tgggcttcgg tgtggcaccc ggcttccaga ccttcgtgtc ctgccaggag
     3781 cagcggctca tctacctacc acctccctgg ggcgactgta aggccacaac gggtgactcg
     3841 gagttctacg acacttacag catcactgcc tgccgcatcg actgcgagac ccgctacctg
     3901 gtggagaact gcaactgccg catggtgcac atgccaggcg atgcccctta ttgcacccca
     3961 gagcagtaca aggagtgtgc agatccagcc ttagatttcc tggtggagaa ggacaatgag
     4021 tactgtgtct gtgagatgcc ctgcaacgtc acccgctatg gcaaagagct ctccatggtg
     4081 aagatcccca gcaaagcctc cgccaagtac ctggcaaaga agtacaacaa gtcggagcag
     4141 tacatcgggg agaacatcct ggtgctggac atcttctttg aagccctgaa ctatgagaca
     4201 atcgagcaga agaaggcgta tgaggtggct ggattgctgg gtgacatcgg gggacagatg
     4261 gggctgttta tcggggccag catcctcact gtgctggagc tctttgacta cgcctatgag
     4321 gtgatcaagc accggctgtg ccggcggggc aaatgccgca agaaccacaa gaggaacaac
     4381 acggacaagg gcgtcgcgct gagcatggat gatgtgaaac gccacaaccc ctgtgagagc
     4441 ctacggggtc acccggctgg catgacgtac gcagcgaaca tcctacctca ccacccggcc
     4501 cggggcacct ttgaggactt cacctgcgca ggatccggac acagcatgat ggacctgaag
     4561 gtggacgagg aggaggtgga cagcgggcag ccggtgagca tccaggcctt cgccagcagc
     4621 tccaccctgc acgggatctc acacatcttc tcctacgagc ggctgtcgct gaagcgcgtg
     4681 gtctgggcgc tctgcttcat gggttcgctg gcgctgctcg ccctggtctg caccaaccgc
     4741 atccagtact acttcctcta cccccacgtc accaagctgg acgaggtggc cgccaccagg
     4801 ctcaccttcc ctgctgtcac cttctgcaac ctcaacgagt tccgcttcag ccgggtgacc
     4861 aagaacgacc tgtaccacgc cggggaactc ctcgccctgc tcaataacag atacgagatc
     4921 ccggacacac agacagctga tgagaagcag ctggaaatcc tacaggacaa ggcaaacttc
     4981 cgaaacttca agcccaaacc tttcaatatg ttggagtttt acgaccgcgc cggccacgac
     5041 atccgggaga tgctgctctc ctgcttcttc cgtggggagc agtgcagccc tgaggacttc
     5101 aaagtggtgt tcacacgcta tgggaagtgc tacacgttca acgcagggca ggatggcaaa
     5161 ccccggctca tcaccatgaa ggggggcact ggaaatggcc tggagatcat gctggacatc
     5221 cagcaggacg agtacctccc agtgtggggg gaaacagatg agacctcatt tgaagccggg
     5281 atcaaggtgc agatccacag ccaggatgag cctccactca tcgaccagct gggcttcggt
     5341 gtggcacccg gcttccagac cttcgtgtcc tgccaggagc agcggctcat ctacctacca
     5401 cctccctggg gcgactgtaa ggccacaacg ggtgactcgg agttctacga cacttacagc
     5461 atcactgcct gccgcatcga ctgcgagacc cgctacctgg tggagaactg caactgccgc
     5521 atggtgcaca tgccaggcga tgccccttat tgcaccccag agcagtacaa ggagtgtgca
     5581 gatccagcct tagatttcct ggtggagaag gacaatgagt actgtgtctg tgagatgccc
     5641 tgcaacgtca cccgctatgg caaagagctc tccatggtga agatccccag caaagcctcc
     5701 gccaagtacc tggcaaagaa gtacaacaag tcggagcagt acatcgggga gaacatcctg
     5761 gtgctggaca tcttctttga agccctgaac tatgagacaa tcgagcagaa gaaggcgtat
     5821 gaggtggctg gattgctggg tgacatcggg ggacagatgg ggctgtttat cggggccagc
     5881 atcctcactg tgctggagct ctttgactac gcctatgagg tgatcaagca ccggctgtgc
     5941 cggcggggca aatgccgcaa gaaccacaag aggaacaaca cggacaaggg cgtcgcgctg
     6001 agcatggatg atgtgaaacg ccacaacccc tgtgagagcc tacggggtca cccggctggc
     6061 atgacgtacg cagcgaacat cctacctcac cacccggccc ggggcacctt tgaggacttc
     6121 acctgctaag atctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     6181 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     6241 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     6301 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gtagacaata
     6361 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     6421 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6481 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6541 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6601 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6661 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6721 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6781 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6841 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     6901 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6961 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7021 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     7081 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7141 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7201 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     7261 atgaggatcg tttcgcatgg tgagcaaggg cgaggaggat aacatggcct ctctcccagc
     7321 gacacatgag ttacacatct ttggctccat caacggtgtg gactttgaca tggtgggtca
     7381 gggcaccggc aatccaaatg atggttatga ggagttaaac ctgaagtcca ccaagggtga
     7441 cctccagttc tccccctgga ttctggtccc tcatatcggg tatggcttcc atcagtacct
     7501 gccctaccct gacgggatgt cgcctttcca ggccgccatg gtagatggct ccggctacca
     7561 agtccatcgc acaatgcagt ttgaagatgg tgcctccctt actgttaact accgctacac
     7621 ctacgaggga agccacatca aaggagaggc ccaggtgaag gggactggtt tccctgctga
     7681 cggtcctgtg atgaccaact cgctgaccgc tgcggactgg tgcaggtcga agaaaactta
     7741 ccccaacgac aaaaccatca tcagtacctt taagtggagt tacaccactg gaaatggcaa
     7801 gcgctaccgg agcactgcgc ggaccaccta cacctttgcc aagccaatgg cggctaacta
     7861 tctgaagaac cagccgatgt acgtgttccg taagacggag ctcaagcact ccaagaccga
     7921 gctcaacttc aaggagtggc aaaaggcctt taccgatgtg atgggcatgg acgagctgta
     7981 caagtgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     8041 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8101 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8161 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8221 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8281 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8521 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10081 cgaccgagtt gct
//
