LOCUS       sequence-468149-       14166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid to produce ENVLPE+ particles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14166)
  AUTHORS   Geilenkeuser J, Armbrust N, Steinmassl E, Du SW, Schmidt S, Binder 
            EMH, Li Y, Warsing NW, Wendel SV, von der Linde F, Schiele EM, Niu 
            X, Stroppel L, Berezin O, Santl TH, Orschmann T, Nelson K, Gruber C,
            Palczewska G, Menezes CR, Risaliti E, Engfer ZJ, Koleci N, Schmidts 
            A, Geerlof A, Palczewski K, Westmeyer GG, Truong DJ
  TITLE     Engineered nucleocytosolic vehicles for loading of programmable 
            editors.
  JOURNAL   Cell. 2025 May 15;188(10):2637-2655.e31. doi: 
            10.1016/j.cell.2025.03.015. Epub 2025 Apr 9.
  PUBMED    40209705
REFERENCE   2  (bases 1 to 14166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2025.03.015"; journalName: "Cell"; date: 
            "2025-05-15- 15"; volume: "188"; issue: "10"; pages: "2637-2655.e31"
COMMENT     Sequence Label: pCMV_ENVLPE+
FEATURES             Location/Qualifiers
     source          1..14166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             340..3351
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    3036..3153
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3925..4158
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4343..4387
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4681..4707
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(4992..5011)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5057..5112
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5111..5130)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5473..5489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5473..5489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5486..5508)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5497..5513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5521..5527,5528..5545,5546..5551))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5566..5587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     enhancer        5664..6043
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6044..6247
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          6359..6931
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     6986..7005
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6986..7004
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7009..7018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8290..8346
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8422..8448
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8461..8487
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             8497..8523
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             8521..8544
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9177..9193)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9207..9224)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9213..9437
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9508..9703
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9554..9689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9709..9843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9746..9765)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9800..9819
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9911..9928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10082..10670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10162..10181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10841..11632,11633..11701))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11464..11483
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11702..11806)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        11853..12232
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12233..12436
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          12548..13120
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             join(13211..14166,1..547)
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
ORIGIN
        1 aaccttgttg gtccaaaatg cgaacccaga ttgtaagact attttaaaag cattgggacc
       61 aggagcgaca ctagaagaaa tgatgacagc atgtcaggga gtggggggac ccggccataa
      121 agcaagagtt ttggctgaag caatgagcca agtaacaaat ccagctacca taatgataca
      181 gaaaggcaat tttaggaacc aaagaaagac tgttaagtgt ttcaattgtg gcaaagaagg
      241 gcacatagcc aaaaattgca gggcccctag gaaaaagggc tgttggaaat gtggaaagga
      301 aggacaccaa atgaaagatt gtactgagag acaggctaat tttttaggga agatctggcc
      361 ttcccacaag ggaaggccag ggaattttct tcagagcaga ccagagccaa cagccccacc
      421 agaagagagc ttcaggtttg gggaagagac aacaactccc tctcagaagc aggagccgat
      481 agacaaggaa ctgtatcctt tagcttccct cagatcactc tttggcagcg acccctcgtc
      541 acaataaaga taggggggca attaaaggaa gctctattag atacaggagc agatgataca
      601 gtattagaag aaatgaattt gccaggaaga tggaaaccaa aaatgatagg gggaattgga
      661 ggttttatca aagtaagaca gtatgatcag atactcatag aaatctgcgg acataaagct
      721 ataggtacag tattagtagg acctacacct gtcaacataa ttggaagaaa tctgttgact
      781 cagattggct gcactttaaa ttttcccatt agtcctattg agactgtacc agtaaaatta
      841 aagccaggaa tggatggccc aaaagttaaa caatggccat tgacagaaga aaaaataaaa
      901 gcattagtag aaatttgtac agaaatggaa aaggaaggaa aaatttcaaa aattgggcct
      961 gaaaatccat acaatactcc agtatttgcc ataaagaaaa aagacagtac taaatggaga
     1021 aaattagtag atttcagaga acttaataag agaactcaag atttctggga agttcaatta
     1081 ggaataccac atcctgcagg gttaaaacag aaaaaatcag taacagtact ggatgtgggc
     1141 gatgcatatt tttcagttcc cttagataaa gacttcagga agtatactgc atttaccata
     1201 cctagtataa acaatgagac accagggatt agatatcagt acaatgtgct tccacaggga
     1261 tggaaaggat caccagcaat attccagtgt agcatgacaa aaatcttaga gccttttaga
     1321 aaacaaaatc cagacatagt catctatcaa tacatggatg atttgtatgt aggatctgac
     1381 ttagaaatag ggcagcatag aacaaaaata gaggaactga gacaacatct gttgaggtgg
     1441 ggatttacca caccagacaa aaaacatcag aaagaacctc cattcctttg gatgggttat
     1501 gaactccatc ctgataaatg gacagtacag cctatagtgc tgccagaaaa ggacagctgg
     1561 actgtcaatg acatacagaa attagtggga aaattgaatt gggcaagtca gatttatgca
     1621 gggattaaag taaggcaatt atgtaaactt cttaggggaa ccaaagcact aacagaagta
     1681 gtaccactaa cagaagaagc agagctagaa ctggcagaaa acagggagat tctaaaagaa
     1741 ccggtacatg gagtgtatta tgacccatca aaagacttaa tagcagaaat acagaagcag
     1801 gggcaaggcc aatggacata tcaaatttat caagagccat ttaaaaatct gaaaacagga
     1861 aagtatgcaa gaatgaaggg tgcccacact aatgatgtga aacaattaac agaggcagta
     1921 caaaaaatag ccacagaaag catagtaata tggggaaaga ctcctaaatt taaattaccc
     1981 atacaaaagg aaacatggga agcatggtgg acagagtatt ggcaagccac ctggattcct
     2041 gagtgggagt ttgtcaatac ccctccctta gtgaagttat ggtaccagtt agagaaagaa
     2101 cccataatag gagcagaaac tttctatgta gatggggcag ccaataggga aactaaatta
     2161 ggaaaagcag gatatgtaac tgacagagga agacaaaaag ttgtccccct aacggacaca
     2221 acaaatcaga agactgagtt acaagcaatt catctagctt tgcaggattc gggattagaa
     2281 gtaaacatag tgacagactc acaatatgca ttgggaatca ttcaagcaca accagataag
     2341 agtgaatcag agttagtcag tcaaataata gagcagttaa taaaaaagga aaaagtctac
     2401 ctggcatggg taccagcaca caaaggaatt ggaggaaatg aacaagtaga taaattggtc
     2461 agtgctggaa tcaggaaagt actattttta gatggaatag ataaggccca agaagaacat
     2521 gagaaatatc acagtaattg gagagcaatg gctagtgatt ttaacctacc acctgtagta
     2581 gcaaaagaaa tagtagccag ctgtgataaa tgtcagctaa aaggggaagc catgcatgga
     2641 caagtagact gtagcccagg aatatggcag ctagtttgta cacatttaga aggaaaagtt
     2701 atcttggtag cagttcatgt agccagtgga tatatagaag cagaagtaat tccagcagag
     2761 acagggcaag aaacagcata cttcctctta aaattagcag gaagatggcc agtaaaaaca
     2821 gtacatacag acaatggcag caatttcacc agtactacag ttaaggccgc ctgttggtgg
     2881 gcggggatca agcaggaatt tggcattccc tacaatcccc aaagtcaagg agtaatagaa
     2941 tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc tgaacatctt
     3001 aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     3061 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     3121 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     3181 ccagtttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt agtaatacaa
     3241 gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcatcag ggattatgga
     3301 aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta acacatggaa
     3361 ttctgcaaca actgctgttt atccatttca gaattgggtg tcgacatagc agaataggcg
     3421 ttactcgaca gaggagagca agaaatggag ccagtagatc ctagactaga gccctggaag
     3481 catccaggaa gtcagcctaa aactgcttgt accaattgct attgtaaaaa gtgttgcttt
     3541 cattgccaag tttgtttcat gacaaaagcc ttaggcatct cctatggcag gaagaagcgg
     3601 agacagcgac gaagagctca tcagaacagt cagactcatc aagcttctct atcaaagcag
     3661 taagtagtac atgtaatgca acctataata gtagcaatag tagcattagt agtagcaata
     3721 ataatagcaa tagttgtgtg gtccatagta atcatagaat ataggaaaat ggccgctgat
     3781 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     3841 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     3901 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     3961 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     4021 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     4081 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     4141 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     4201 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     4261 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     4321 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     4381 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     4441 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     4501 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     4561 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     4621 attagtgaac ggatccttgg cacttatctg ggacgatctg cggagcctgt gcctcttcag
     4681 ctaccaccgc ttgagagact tactcttgat tgtaacgagg attgtggaac ttctgggacg
     4741 cagggggtgg gaagccctca aatattggtg gaatctccta caatattgga gtcaggagct
     4801 aaagaatagt gctgttagct tgctcaatgc cacagccata gcagtagctg aggggacaga
     4861 tagggttata gaagtagtac aaggagcttg tagagctatt cgccacatac ctagaagaat
     4921 aagacagggc ttggaaagga ttttgctata agctcgaaac aaccggtacc tctagaacta
     4981 tagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     5041 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     5101 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     5161 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     5221 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     5281 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     5341 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     5401 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     5461 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5521 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5581 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     5641 gatccggcgc gccccgccat gttgacattg attattgact agttattaat agtaatcaat
     5701 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     5761 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     5821 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     5881 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     5941 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     6001 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     6061 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     6121 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     6181 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     6241 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     6301 ccatagaaga caccgggacc gatccagcct ccggtcgacc gatcctgaga acttcagggt
     6361 gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg
     6421 agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg
     6481 accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct
     6541 tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat
     6601 ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt
     6661 tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat
     6721 aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct
     6781 tattggtaga aacaactaca ccctggtcat catcctgcct ttctctttat ggttacaatg
     6841 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
     6901 ccatgttcat gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct
     6961 gtctcatcat tttggcatta attaataata cgactcacta taggggccgc caccatgggc
     7021 gctagagcct ctgttctgtc tggcggcgaa ctggacagat gggagaagat cagaagcaga
     7081 cctggcggca agaagaagta caagctgaag cacatcgtgt gggccagcag agagctggaa
     7141 agattcgctg tgaaccccgg cctgctggaa acaagcgagg gctgtagaca gatcctgggc
     7201 cagctccagc catctctcca gacaggatct gaggaactga gaagcctgta caacaccgtg
     7261 gccacactgt actgcgtgca ccagagaatc gagatcaagg acaccaaaga ggccctggac
     7321 aagatcgagg aagaacagaa caagtctaag aagaaggccc agcaggctgc cgccgataca
     7381 ggccactcta atcaggtgtc ccagaactac cccatcgtgc agaacatcca gggccagatg
     7441 gtgcatcagg ctatcagccc cagaacactg aacgcctggg tcaaagtggt ggaagagaag
     7501 gctttcagcc ctgaagtgat ccccatgttc agcgccctgt ctgagggtgc cacacctcag
     7561 gacctgaaca ccatgctgaa cacagtcggc ggacaccagg ccgctatgca gatgctgaaa
     7621 gagacaatca acgaagaggc cgccgagtgg gacagagtgc atcctgttca tgctggccct
     7681 atcgctcccg gccagatgag agaacctaga ggctctgaca ttgccggcac caccagcaca
     7741 ctgcaagagc agatcggctg gatgacaaac aaccctccta ttcctgtggg cgagatctac
     7801 aagagatgga tcatcctggg actgaacaag atcgtgcgga tgtacagccc caccagcatc
     7861 ctggacatta gacagggccc caaagagccc ttcagagact acgtggacag attctacaag
     7921 accctgagag ccgagcaggc cagccaagaa gtgaagaact ggatgaccga gacactgctg
     7981 gtgcagaacg ctaaccccga ctgcaagacc atcctgaagg ctctgggacc tgccgccaca
     8041 ctggaagaga tgatgacagc ctgtcaaggc gtcggaggac ctggccataa ggctagagtt
     8101 ctggccgagg ccatgagcca agtgaccaac agcgccacaa tcatgatgca gagaggcaac
     8161 ttcagaaacc agcggaagat cgtgaagtcc ttcaactccg gcaaagaggg ccacaccgcc
     8221 cgcaattcta gagcccctag aaagaaaggt ggatccggcg gcagaatgaa gcagctggaa
     8281 gataaggtgg aagaactgct gagcaagaac taccacctgg aaaacgaggt ggcccggctg
     8341 aagaagctcg ttggagaaag aggtggcagc ggcggagttt ctggatggcg gctgttcaag
     8401 aagatcagcg gcggttccgg acctgctgcc aaaagagtga aactggactc cggactgcaa
     8461 ctgcctccac tggaaagact gacactggga gctgccccag ccgccaagaa gaaaaagctg
     8521 gactacaagg acgacgacga caagggcgga gctagctcta agacaatcgt gctgtctgtg
     8581 ggcgaagcca ccagaacact gaccgagatc cagagcacag ccgacagaca gatcttcgag
     8641 gaaaaagtgg gccctctcgt gggcagactg agactgacag cttccctgag acagaacggc
     8701 gccaagaccg cctacagagt gaacctgaag ctggaccagg ccgacgtggt ggattctggc
     8761 cttcctaaag tgcggtacac ccaagtgtgg tcccacgacg tgaccatcgt ggccaattct
     8821 accgaggcca gcagaaagag cctgtacgac ctgaccaaga gcctggtggc cacaagccag
     8881 gtggaagatc tggtcgtgaa cctggtgcct ctcggacgtg gcggatccgg tggaaccgag
     8941 agacaggcca acttcctggg caagatctgg cctagctaca agggcagacc cggcaacttc
     9001 ctccagagca gaccagagcc taccgctcct cctgaggaat ccttcagatc cggcgtggaa
     9061 accaccacac ctccacagaa gcaagagccc atcgacaaag agctgtaccc tctgaccagc
     9121 ctgagatccc tgttcggcaa cgaccctagc tctcaatgaa cgcgtaaatg attgcagatc
     9181 cactagttct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     9241 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     9301 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     9361 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     9421 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga gatccactag
     9481 ttctagcctc gaggctagag cggccgcgca tctcaattag tcagcaacca tagtcccgcc
     9541 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9601 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9661 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
     9721 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9781 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     9841 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9901 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9961 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    10021 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    10081 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    10141 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    10201 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10261 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10321 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10381 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10441 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10501 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10561 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10621 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10681 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10741 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10801 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10861 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10921 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10981 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    11041 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    11101 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    11161 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    11221 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    11281 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    11341 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11401 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11461 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11521 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11581 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11641 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11701 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11761 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11821 aagtgccacc tgggtccccg ggccgccatg ttgacattga ttattgacta gttattaata
    11881 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
    11941 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
    12001 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
    12061 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
    12121 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
    12181 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
    12241 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
    12301 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
    12361 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
    12421 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
    12481 ttttgacctc catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa
    12541 cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat
    12601 gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta
    12661 tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt
    12721 gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt
    12781 gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc
    12841 actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac
    12901 aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg
    12961 aaatattctt attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg
    13021 gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc
    13081 ctctgctaac catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt
    13141 tgttgtgctg tctcatcatt ttggcagcga tcgcgcacgg caagaggcga ggggcggcga
    13201 ctggtgagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgatggga
    13261 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
    13321 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
    13381 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
    13441 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
    13501 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagaaaa aagcacagca
    13561 agcagcagct gacacaggac acagcaatca ggtcagccaa aattacccta tagtgcagaa
    13621 catccagggg caaatggtac atcaggccat atcacctaga actttaaatg catgggtaaa
    13681 agtagtagaa gagaaggctt tcagcccaga agtgataccc atgttttcag cattatcaga
    13741 aggagccacc ccacaagatt taaacaccat gctaaacaca gtggggggac atcaagcagc
    13801 catgcaaatg ttaaaagaga ccatcaatga ggaagctgca gaatgggata gagtgcatcc
    13861 agtgcatgca gggcctattg caccaggcca gatgagagaa ccaaggggaa gtgacatagc
    13921 aggaactact agtacccttc aggaacaaat aggatggatg acacataatc cacctatccc
    13981 agtaggagaa atctataaaa gatggataat cctgggatta aataaaatag taagaatgta
    14041 tagccctacc agcattctgg acataagaca aggaccaaag gaacccttta gagactatgt
    14101 agaccgattc tataaaactc taagagccga gcaagcttca caagaggtaa aaaattggat
    14161 gacaga
//
