LOCUS       sequence-468150-        3429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine 
            mutation for disulfide linkage to mutant beta-2 microglobulin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Sun Y, Young MC, Woodward CH, Danon JN, Truong HV, Gupta S, Winters 
            TJ, Font-Burgada J, Burslem GM, Sgourakis NG
  TITLE     Universal open MHC-I molecules for rapid peptide loading and 
            enhanced complex stability across HLA allotypes.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jun 20;120(25):e2304055120. doi: 
            10.1073/pnas.2304055120. Epub 2023 Jun 13.
  PUBMED    37310998
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-06-20"; volume: "120(25):e2304055120. 
            doi"; pages: " 10.1073/pnas.2304055120. Epub 2023 Jun 13"
COMMENT     Sequence Label: OPEN-HLA-A*11:01-BirA
FEATURES             Location/Qualifiers
     source          1..3429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..61)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(721..1512,1513..1581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1344..1363
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1582..1686)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1754..1772
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1817..1864)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1860..1878
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     RBS             2833..2855
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(2886..2905)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2887..2905)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2958..2980)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3066..3205
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3121..3140
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3220..3237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3391..3429,1..550))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
       61 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      121 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      181 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      241 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      301 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      361 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
      421 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      481 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      541 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      601 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      661 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
      721 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
      781 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      841 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      901 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      961 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1021 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1081 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1141 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1201 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1261 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1321 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     1381 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     1441 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     1501 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     1561 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     1621 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     1681 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1741 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tatccggata
     1801 tagttcctcc tttcagcaaa aaacccctca agacccgttt agaggcccca aggggttatg
     1861 ctagttattg ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag
     1921 ccggatcgga attcaagctt aatgccattc gattttctga gcttcaaaaa tatcgttcag
     1981 accaccaccg gatcccggct cccatctcag ggtgaggggc ttgggcagac cctcatgctg
     2041 cacatggcag gtgtatctct gctcctctcc agaaggcacc accacagccg cccacttctg
     2101 gaaggttcca tcccctgcag gcctggtctc cacgagctcc gtgtcctggg tctggtcctc
     2161 cccatcccgc tgccaggtca gtgtgatctc cgcagggtag aagcccaggg cccagcacct
     2221 cagggtggcc tcatggtcag agatggggtg gtgggtcata tgtgtcttgg gggggtccgt
     2281 gcgctgcagc gtctccttcc cgttctccag gtatctgcgg agccactcca cgcaccggcc
     2341 ctccaggtag gctctctgct gctccgccgc atgggccgcc tcccacttgc gcttggtgat
     2401 ctgagctgcc atgtccgccg cggtccaaga gcgcaggtcc tcgttcaggg cgatgtaatc
     2461 cttgcagtcg taggcgtcct gccggtaccc gcggaggaag cgcccgtccg gccccacgtc
     2521 gcagccatac attatctgga tggtgtgaga accgtcctcg ctctggttgt agtagccgcg
     2581 cagggtcccc aggtccactc ggtcagtctg tgactgggcc ttcacattcc gtgtctcctg
     2641 gtcccaatac tccggcccct cctgctctat ccacggcgcc cgcggctcca tcctctggct
     2701 cgcggcgtcg ctgtcgaacc gcacgaactg cgtgtcgtcc acgtagccca cggcgatgaa
     2761 gcggggctcc ccgcggccgg gccgggacac ggaggtgtag aaatacctca tagaatgaga
     2821 acccattatg tatctccttc ttaaagttaa acaaaattat ttctagaggg aaaccgttgt
     2881 ggtctcccta tagtgagtcg tattaatttc gcgggatcga gatcctgcct cgcgcgtttc
     2941 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     3001 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     3061 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     3121 cggcatcaga gcagattgta ctgagagtgc accattgcgg tgtgaaatac cgcacagatg
     3181 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3241 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3301 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3361 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3421 tcacaaaaa
//
