LOCUS       sequence-468151-        8940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mNeonGreen-tagged PH domain of PLCD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Nikolatou K, Sandilands E, Roman-Fernandez A, Cumming EM, Freckmann 
            E, Lilla S, Buetow L, McGarry L, Neilson M, Shaw R, Strachan D, 
            Miller C, Huang DT, McNeish IA, Norman JC, Zanivan S, Bryant DM
  TITLE     PTEN deficiency exposes a requirement for an ARF GTPase module for 
            integrin-dependent invasion in ovarian cancer.
  JOURNAL   EMBO J. 2023 Sep 18;42(18):e113987. doi: 10.15252/embj.2023113987. 
            Epub 2023 Aug 14.
  PUBMED    37577760
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-09-18"; volume: "42(18):e113987. doi"; pages: " 
            10.15252/embj.2023113987. Epub 2023 Aug 14"
COMMENT     Sequence Label: pLX304 mNG PH PLCD1
FEATURES             Location/Qualifiers
     source          1..8940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(384..395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             573..806
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    884..1005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(921..940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     975..994
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1045..1180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1200..1219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1201..1219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1229..1246)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1229..1245)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1238..1260)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1387..1842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1474..1493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1684..1705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1868..1972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1973..2041,2042..2833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2191..2210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3004..3592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3493..3512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3746..3763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3880..3901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3916..3921,3922..3939,3940..3946)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3954..3970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3959..3981
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3978..3994
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3978..3994
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4013..4033
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4015..4033
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4061..4287
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4288..4468
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4515..4640
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5133..5366
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5551..5595
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5770..6270
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6189..6207
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6282..6677
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6737..6854
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6971..7274
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7275..7478
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7429..7449
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      8072..8081
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8078..8782
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELY"
     regulatory      8333..8342
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8793..8810
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(8853..8940,1..501)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8906..8926)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
       61 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
      121 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
      181 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
      241 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
      301 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
      361 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
      421 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
      481 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
      541 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
      601 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
      661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      781 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
      841 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
      901 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
      961 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     1021 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     1081 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     1141 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     1201 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     1261 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1321 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1381 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1441 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1501 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1561 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1621 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1681 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1741 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1801 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     1861 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1921 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1981 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2041 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2101 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2161 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2221 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2281 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2341 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2401 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2461 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2521 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2581 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2641 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2701 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2761 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2821 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2881 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2941 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3001 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3061 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3121 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3181 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3241 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3301 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3361 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3421 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3481 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3541 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3601 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3661 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3721 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3781 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3841 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     3901 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     3961 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     4021 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     4081 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     4141 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     4201 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     4261 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     4321 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4381 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4441 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     4501 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     4561 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     4621 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     4681 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     4741 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     4801 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     4861 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     4921 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     4981 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     5041 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     5101 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     5161 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     5221 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     5281 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     5341 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     5401 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     5461 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     5521 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     5581 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     5641 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     5701 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     5761 aggggaccct tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     5821 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     5881 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     5941 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     6001 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     6061 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     6121 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     6181 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     6241 gttgaccgaa tcaccgacct ctctccccag ggggtaccac catggccaag cctttgtctc
     6301 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     6361 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     6421 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     6481 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     6541 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
     6601 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     6661 gttatgtgtg ggagggcctg cagctgcagt agtaagaatt ctagatcttg agacaaatgg
     6721 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     6781 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     6841 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
     6901 agggggcccg gggttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     6961 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     7021 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     7081 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     7141 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     7201 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     7261 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     7321 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     7381 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     7441 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
     7501 gctagcgcta ccggactcag atctcgagct caagcttcga attcacggca tggactcggg
     7561 ccgggacttc ctgaccctgc acggcctaca ggatgatgag gatctacagg cgctgctgaa
     7621 gggcagccag ctcctgaagg tgaagtccag ctcatggagg agagagcgct tctacaagtt
     7681 gcaggaggac tgcaagacca tctggcagga gtcccgcaag gtcatgcgga ccccggagtc
     7741 ccagctgttc tccatcgagg acattcagga ggtgcgaatg gggcaccgca cggagggtct
     7801 ggagaagttc gcccgtgatg tgcccgagga ccgctgcttc tccattgtct tcaaggacca
     7861 gcgcaataca ctagacctca tcgccccatc gccagctgat gcccagcact gggtgctggg
     7921 gctgcacaag atcatccacc actcaggctc catggaccag cgtcagaagc tacagcactg
     7981 gattcactcc tgcttgcgaa aagctgacaa aaacaaggac aacaagatga gcttcaagga
     8041 gctgcagaac ttcctgaagg atccaccggt cgccaccatg gtgagcaagg gcgaggagga
     8101 taacatggcc tctctcccag cgacacatga gttacacatc tttggctcca tcaacggtgt
     8161 ggactttgac atggtgggtc agggcaccgg caatccaaat gatggttatg aggagttaaa
     8221 cctgaagtcc accaagggtg acctccagtt ctccccctgg attctggtcc ctcatatcgg
     8281 gtatggcttc catcagtacc tgccctaccc tgacgggatg tcgcctttcc aggccgccat
     8341 ggtagatggc tccggctacc aagtccatcg cacaatgcag tttgaagatg gtgcctccct
     8401 tactgttaac taccgctaca cctacgaggg aagccacatc aaaggagagg cccaggtgaa
     8461 ggggactggt ttccctgctg acggtcctgt gatgaccaac tcgctgaccg ctgcggactg
     8521 gtgcaggtcg aagaagactt accccaacga caaaaccatc atcagtacct ttaagtggag
     8581 ttacaccact ggaaatggca agcgctaccg gagcactgcg cggaccacct acacctttgc
     8641 caagccaatg gcggctaact atctgaagaa ccagccgatg tacgtgttcc gtaagacgga
     8701 gctcaagcac tccaagaccg agctcaactt caaggagtgg caaaaggcct ttaccgatgt
     8761 gatgggcatg gacgagctgt acattaatta accaccacca ccaccaccac taaggatccc
     8821 gtacgtgatc accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     8881 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
//
