LOCUS       sequence-468153-        8116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cellular colocalization assay (PYRMandi/ABI) for the CIP Mandi 
            (mandipropamid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Poschko P, Berrou CM, Pakari K, Ziegler MJ, Kern C, Koch B, 
            Wittbrodt J, Wombacher R
  TITLE     Photoactivatable Plant Hormone-Based Chemical Inducers of Proximity 
            for In Vivo Applications.
  JOURNAL   ACS Chem Biol. 2025 Jan 27. doi: 10.1021/acschembio.4c00592.
  PUBMED    39868662
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2025 Jan 27. doi: 10.1021/acschembio.4c00592."
COMMENT     Sequence Label: pPYRMandi-ABI
FEATURES             Location/Qualifiers
     source          1..8116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1034..1741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1179..1197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1447..1466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2339..2395
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2405..2407,2408..2410,2411..3121)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2405..2407,2408..2410,2411..3121)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2450..2471)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2711..2730)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3058..3079
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4068..4085)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4074..4298
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4431..4450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4582..4629
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4637..5659
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4637..5659
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5789..5910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5826..5845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5880..5899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5959..5975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5959..5975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5972..5994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5983..5999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6007..6013,6014..6031,6032..6037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6052..6073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6190..6207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6361..6949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6441..6460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7120..7911,7912..7980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7743..7762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7981..8085)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt accaccatgg gtcggaacag cgccatcgcc gcgggcgtgt gcggtgccct
      961 cttcataggg tactgcatct actttgaccg caaaagacga agtgacccca acttcggatc
     1021 cggagcaagt ggaatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1081 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1141 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1201 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1261 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1321 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1381 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1441 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1501 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     1561 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     1621 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     1681 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg aactgtacaa
     1741 gagcgcagga ggaccaatgc catctgaatt gacccctgag gaacgctccg aattgaaaaa
     1801 ttcaatcgcc gaattccata cctatcagct cgaccccgga tcttgcagtt cactgcatgc
     1861 acagcgcatc cacgcgcccc cagaattggt gtggtctatc gttcgccgct ttgacaaacc
     1921 ccaaacgcac cggcacttca taaagtcatg ttcagttgaa cagaatttcg aaatgcgagt
     1981 gggctgcacc agagatataa tagtaatatc cggtctccct gcaaatacat ccacggagcg
     2041 actggacata cttgacgatg aaagaagagt tacgggcgct tctataattg ggggcgaaca
     2101 ccggctgact aactataagg gcgtcacaac cgttcaccgc ttcgagaagg aaaaccgcat
     2161 ctggactgta gtgttggaaa gctatgtagt ggatatgcct gaaggaaatt ctgaagacga
     2221 cactaggatg cttgcggata cagtcgtcaa acttaacctc cagaaacttg ctactgtagc
     2281 ggaggctatg gcccggaact caggtgatgg ctctggcagc caggtcacgg gctctggtgc
     2341 cacaaacttc tctctgctaa agcaagcagg tgatgttgaa gaaaaccccg ggcctggcgg
     2401 ttctatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2461 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2521 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2581 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2641 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2701 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2761 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2821 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2881 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2941 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3001 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3061 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3121 gagcgcagga ggaacgcgtg tgcctttgta tggttttact tcgatttgtg gaagaagacc
     3181 tgagatggaa gctgctgttt cgactatacc aagattcctt caatcttcct ctggttcgat
     3241 gttagatggt cggtttgatc ctcaatccgc cgctcatttc ttcggtgttt acgacggcca
     3301 tggcggttct caggtagcga actattgtag agagaggatg catttggctt tggcggagga
     3361 gatagctaag gagaaaccga tgctctgcga tggtgatacg tggctggaga agtggaagaa
     3421 agctcttttc aactcgttcc tgagagttga ctcggagatt gagtcagttg cgccggagac
     3481 ggttgggtca acgtcggtgg ttgccgttgt tttcccgtct cacatcttcg tcgctaactg
     3541 cggtgactct agagccgttc tttgccgcgg caaaactgca cttccattat ccgttgacca
     3601 taaaccggat agagaagatg aagctgcgag gattgaagcc gcaggaggga aagtgattca
     3661 gtggaatgga gctcgtgttt tcggtgttct cgccatgtcg agatccattg gcgatagata
     3721 cttgaaacca tccatcattc ctgatccgga agtgacggct gtgaagagag taaaagaaga
     3781 tgattgtctg attttggcga gtgacggggt ttgggatgta atgacggatg aagaagcgtg
     3841 tgagatggca aggaagcgga ttctcttgtg gcacaagaaa aacgcggtgg ctggggatgc
     3901 atcgttgctc gcggatgagc ggagaaagga agggaaagat cctgcggcga tgtccgcggc
     3961 tgagtatttg tcaaagctgg cgatacagag aggaagcaaa gacaacataa gtgtggtggt
     4021 ggttgatttg aagtagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4081 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4141 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4201 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4261 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4321 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4381 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4441 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4501 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4561 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     4621 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     4681 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     4741 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     4801 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     4861 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     4921 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     4981 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     5041 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     5101 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     5161 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     5221 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     5281 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     5341 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     5401 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     5461 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     5521 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     5581 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     5641 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     5701 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5761 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     5821 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5881 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     5941 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6001 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6061 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6121 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6181 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6241 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6961 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7021 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7081 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7141 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7201 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7261 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7321 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7381 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7441 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7501 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7561 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7621 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7681 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7741 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7801 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7861 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7921 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7981 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8041 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8101 agtgccacct gacgtc
//
