LOCUS       sequence-46817-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Parker JB, Palchaudhuri S, Yin H, Wei J, Chakravarti D
  TITLE     A Transcriptional Regulatory Role of the THAP11-HCF-1 Complex in 
            Colon Cancer Cell Function.
  JOURNAL   Mol Cell Biol. 2012 May;32(9):1654-70. Epub 2012 Feb 27.
  PUBMED    22371484
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2012-05"; volume: "32(9)"; pages: "1654-70. Epub 2012
            Feb 27"
COMMENT     Sequence Label: pSUPER.retro.puro-THAP11-A
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..59
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     44..60
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(132..346)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(192..211)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        357..856
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(392..415)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     789..808
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(881..900)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tagccctact ccttctctag gcgccggaat tagatcgatc tctcgaggtc gacggtatcg
       61 ataagctttt ccaaaaaagc gaagaactgc gtgagaatct cttgaattct cacgcagttc
      121 ttcgcggatc tgtggtctca tacagaactt ataagattcc caaatccaaa gacatttcac
      181 gtttatggtg atttcccaga acacatagcg acatgcaaat attgcagggc gccactcccc
      241 tgtccctcac agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc cgcctagtga
      301 cactgggccc gcgattcctt ggagcgggtt gatgacgtca gcgttcgaat tctaccgggt
      361 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
      421 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
      481 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
      541 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
      601 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
      661 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
      721 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
      781 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
      841 atctccgggc ctttcgacct gcagcccaag ctagcttacc atgaccgagt acaagcccac
      901 ggtgcgcctg acg
//
