LOCUS       sequence-468185-        8922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz 
            L, Nusse R, Weissman IL
  TITLE     A role for Wnt signalling in self-renewal of haematopoietic stem 
            cells.
  JOURNAL   Nature. 2003 May 22. 423(6938):409-14.
  PUBMED    12717450
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 May 22. 423(6938):409-14."
COMMENT     Sequence Label: gag/pol
FEATURES             Location/Qualifiers
     source          1..8922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(91..96,97..114,115..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     153..169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    280..414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(317..336)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     371..390
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(429..4028,4029..4031,4032..5645))
                     /codon_start=1
                     /gene="gag-pol"
                     /product="Moloney murine leukemia virus Gag-Pol 
                     polyprotein"
                     /label=gag-pol
                     /note="contains a read-through stop codon"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPT
                     FNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPK
                     PPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTEDPP
                     PYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLRAGGN
                     GQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGE
                     EKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAG
                     LQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIW
                     QSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTE
                     DEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDC
                     PKKPRGPRGPRPQTSLLTLDD*GGQGQEPPPEPRITLKVGGQPVTFLVDTGAQHSVLTQ
                     NPGPLSDKSAWVQGATGGKRYRWTTDRKVHLATGKVTHSFLHVPDCPYPLLGRDLLTKL
                     KAQIHFEGSGAQVMGPMGQPLQVLTLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAE
                     TGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPW
                     NTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKD
                     AFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDLADFRI
                     QHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGY
                     LLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAPLYPLTKT
                     GTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWR
                     RPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPP
                     DRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRP
                     DLTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIAL
                     TQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGLLTSEGKEIKNKDEILALLKALF
                     LPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSPYTSEHFH
                     YTVTDIKDLTKLGAIYDKTKKYWVYQGKPVMPDQFTFELLDFLHQLTHLSFSKMKALLE
                     RSHSPYYMLNRDRTLKNITETCKACAQVNASKSAVKQGTRVRGHRPGTHWEIDFTEIKP
                     GLYGYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFV
                     SKVSQTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLA
                     LYRARNTPGPHGLTPYEILYGAPPPLVNFPDPDMTRVTNSPSLQAHLQALYLVQHEVWR
                     PLAAAYQEQLDRPVVPHPYRVGDTVWVRRHQTKNLEPRWKGPYTVLLTTPTALKVDGIA
                     AWIHAAHVKAADPGGGPSSRLTWRVQRSQNPLKIRLTREAP"
     misc_feature    492..866
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(538..554)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(5242..5258)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5274..5296)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     regulatory      5642..5651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          5712..5808
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        complement(5854..6115)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(6475..6492)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6475..6491)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6484..6506)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6819..6841
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6879..6897)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6965..7069
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7070..7138,7139..7930)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7288..7307)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8101..8689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8590..8609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8843..8860
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      181 caagcttgca tgcctgcagg tcgactctag aggatccgaa aaaacctccc acacctcccc
      241 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      301 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      361 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccgcg
      421 gccgctcatt agggggcctc gcgggttaac cttattttta aggggttttg agagcgttga
      481 acgcgccatg tcagtctaga ggatggtcca cccccggggt cggcagcctt cacgtgggcg
      541 gcgtgtatcc aagctgcgat gccgtctact ttgagggcgg tgggggtggt cagcaggact
      601 gtgtaaggtc ctttccagcg aggttctagg ttcttagtct ggtgtcggcg gacccacact
      661 gtgtcgccga ctcggtaagg gtgaggtacc accggtcggt ccagttgttc ttggtaggct
      721 gccgccagag gtctccagac ttcgtgctgg actaagtaga gagcctgtaa gtgagcttgg
      781 agagaggggc tgttagtaac tcttgtcatg tcagggtcag ggaagtttac aaggggcggg
      841 ggtgccccat ataagatctc atatggggtg aggccatggg ggcccggcgt gttgcgggct
      901 cggtacaggg ctaaggggag taggagcacc cagtctctag agccagttgc aagcgttaat
      961 ttagttaaag tctccttgat ggttctattc attctttcta cctggcctga gctttggggt
     1021 ctgtatgcac aatgtaattt ccaatcaatc cccaacagat cggccactgt ctgactcacc
     1081 ttggagacga aggcaggccc attgtcagtt cccaatacct gaggcatgcc gaacctgggg
     1141 aagatctcct ctagtagctt cttggttacg accttggcgg tttctttctt ggttgggaag
     1201 gcttctatcc agccagaaaa ggtatctata aaaactagaa gatatttata gccatacaat
     1261 ccgggcttta tctcggtgaa atcgatctcc caatgagtgc cgggccgatg cccgcggacc
     1321 ctagttccct gtttaacggc agacttgctg gcgttgactt gtgcacaagc tttgcaggtc
     1381 tcagtgatat ttttgagtgt tcgatcccgg ttcagcatgt agtagggact gtggcttctc
     1441 tctaggagag ccttcatttt tgagaagctg aggtgagtca gctgatgaag aaagtctaat
     1501 aattcaaaag taaactggtc aggcatcaca ggttttcctt ggtagaccca atacttcttt
     1561 gttttatcat aaatggcccc caacttggtt aggtccttta tatcagtcac tgtgtaatga
     1621 aaatgttctg aggtgtaggg tgatgaattt tctatgagga gggtagaggt gtctggagtc
     1681 tctgtgatgg ctgcctttcg ggccgcttgg tcagccatcc ggttgcctct agcctcggcg
     1741 ctgtgtccct tttgatgtcc tggacaatgg attatgctaa gtcttttggg cagaaagagg
     1801 gcttttagta gggccaagat ctcgtcttta tttttgatct ctttgccttc tgatgtgagc
     1861 aacccacgcc ttctgtatat ttctccatgg atatgggcag tagcaaaagc ataacggcta
     1921 tcagtataaa catttagctt cttaccttct gccatcttta gggcctgggt gagtgctatc
     1981 agttcagccc gctgagcgga tgtcccggct ggcagggctt tagcccagat tacctcggtc
     2041 tcggtggtca ccgcagctcc cgccttacgc tgtccctctt gtaagagact gcttccatcc
     2101 gtgtaccagg tgtggtcggc gtctgggagc ggctggtccg ttaggtcggg tcgggttccg
     2161 tgggcttcgg ccaggatatc aaggcagttg tgttgcagcc cttcctcagg cagtgggagc
     2221 agcgtagccg ggttcagggc taccaccggt ccgaactgga cccggtccgt gtccaaaagc
     2281 aaggcctgat agtgagtcat ccgggcgttg gaaagccagc ggtcgggggg ttgtttgact
     2341 agtgcctcta ctgcatgggg ggccagaatg actagtggct gtcccatggt tagcttgcct
     2401 gcatcctttg tcagtacggc aatggctgct accatccgta ggcaaggggg ccacccagct
     2461 gctactgggt ctagcttttt ggacaggtag gccaccggcc gacgccaagg tcccagtttt
     2521 tgcgttagga cacctttggc gtagccctgc ttctcgtcga caaagagttc aaagggctta
     2581 gtcaaatctg gcaaccccag ggctggggca gttagaagag cttgcttgat ttcttgatag
     2641 gccttttgtt ggtctgggcc ccaattaaac agagtccccg ttttggtgag agggtacaag
     2701 ggggctgcca tttctgcaaa cccagggatc cagaggcgac agaagcctgc cgtccctagg
     2761 aactccctta gttgtcgagg ggtcttcgga gtaggctgcc ccatcacagt ctcttttctg
     2821 gcctcagtca gccatctctg accctctttt agaagatacc ccagatactt gacctgtttc
     2881 tggcaaattt gggctttctt ggccgaggcc cgatacccga ggttccctag ggtttgtaac
     2941 agggcccgag taccttgttg gcagtctagc tcagaagtgg cggccagcag taagtcatcc
     3001 acgtactgta gcaggatcaa gtctgggtgc tggatccgga agtctgctag gtctctgtgc
     3061 agtgcctcat caaacagggt gggactgttt ttgaaaccct gtgggagtct ggtccaggtc
     3121 aattgtcctg agattcccat ctctggatct ctccactcaa aggcgaagag aggctgactg
     3181 gtggggtgga gtctcaggca gaaaaaggca tcctttaaat caagcacagt gtaccactgg
     3241 tgggacggtg ggagcccgct caagaggttg taagggttgg gcacggtggg gtggatgtct
     3301 tccacccgct tgttgacttc tctcagatcc tggacaggcc tataatcatt agtccctggt
     3361 ttcttaacgg gtagcagggg cgtgttccag ggggactggc agggtaccag tattccctgg
     3421 tccaacagtc tctgtatgtg gggcttgatc cccagtctgg cttcttgtga catggggtat
     3481 tgttttatgg acacgggggt agaggttgct ttcagaggta tgatcagagg agcttggcga
     3541 actgccagtc ccatgccccc ggtttccgcc caggcctgag gaaaatcaga cagccatgtg
     3601 gaccctagag aaacatctgg ctcttttgag gtctcatgta gccgatactc atcttctata
     3661 tttagggtca acacttgcag gggctgcccc attggtccca taacctgagc tcctgatccc
     3721 tcaaagtgga tttgggcttt tagtttagtc agcaaatctc ttcctaacag aggataggga
     3781 cagtctggta catggaggaa agagtgggtg accttaccgg tagctagatg tactttgcga
     3841 tccgtggtcc agcgataccg ctttcctcca gtagcccctt ggacccaggc agacttatca
     3901 cttaggggtc caggattttg ggtcagcacg gagtgttggg ccccagtatc taccaggaag
     3961 gtgacgggtt gccccccgac tttgagggtt atcctgggtt cagggggggg ctcctgaccc
     4021 tgacctccct agtcatctag ggtcaggagg gaggtctggg gtcttggtcc ccgaggtcct
     4081 cgtggtttct tgggacaatc tttagcccag tgcccctttt ctttgcagta ggcacactgg
     4141 tcgcgatcga gttgggacct ccttcgttct cctccctgtc tatcctgttt ctgtccacta
     4201 acgacagtgg ccaatagctt gctcatctct ctatgtctcc tacgatctct ttctttctct
     4261 ttctgctcat cctctgtcct acggcgttct tctttttcct ctgtttctct cctgatacgt
     4321 tcctctcttt cttccggggt ttctcgttta ttaaagatct tttctgcctc tctaaccaaa
     4381 tctccaagcg tcttgttttt taaatcttct aacctctcta actttctccc aatgtctggg
     4441 gcagactgcc aaatgaaaga catagacaca ttagtttctt gccctgggtc ctcagggtca
     4501 taaggagtgt acctgcgata ggcttcctta agtctctcta ggaaggccga gggagactca
     4561 ttgggccctt gtgttattcc ttttaccttg gccaaattgg tggggcttct gcccgcgttt
     4621 tggagacccg ctaggagcaa ctggcgatag tggactaggt ggttcctacc tgcctgggtg
     4681 gtgtaatccc agtctgggcg ctcgagggga aaagcggcat cgacttcatt gggcagttga
     4741 gtggggcgcc catcatcgcc ccgcaccgcc tttctagcct ctaagagcac ccgttgtttt
     4801 tcttctccgg tcagcagagt ccccaacagc tgctgacagt cgtcccaggt gggctgatgg
     4861 gtgatgagaa cagactcgat cagagctgtc agtttacctg gatcttcaga aaaagaaggg
     4921 ttattatttt tccagttgta aaggtcagaa gaggagaacg gccagtattg aagctgtccg
     4981 tttcctcctg cgcggagggg gaatgcctgc gaggtagtgg agtcggccac agggggctcc
     5041 cgtctcccac gtaggcgaga tgccattggg gaggggtccg gtgcctctcc cgcaggggtc
     5101 gcttctccac catttccgtc cctgtcggaa gggggtggtc ttgggtccct ataaggcggg
     5161 gggtcttctg taagtaggtc gatgagcggc cccccactgt cagaaagaac ttgaggttta
     5221 ggtttggcgc ctagagaagg agtgagggct ggataaaggg aggatcgagg cggggtcgaa
     5281 cgaggaggtt caagggggag agacggggcg gatggaggaa gaggaggcgg aggcttaggg
     5341 tgtacaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca ggtcacgatg
     5401 taggggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat cttaacctgg
     5461 gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa ggttggccat
     5521 tctgcagagc agaaggtaac ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg
     5581 atccgctcga catctttcca gtgacctaag gtcaaactta agggagtggt aacagtctgg
     5641 cccatggtgg cggcgcggcc gcgggtacaa ttccgcagct tttagagcag aagtaacact
     5701 tccgtacagg cctagaagta aaggcaacgt ccactgagga gcagttcttt gatttgcacc
     5761 accaccggat ccgggacctg aaataaaaga caaaaagact aaacttacca gttaactttc
     5821 tggtttttca gttcctcgag tacccagctt ggaggtgcac accaatgtgg tgaatggtca
     5881 aatggcgttt attgtatcga gctaggcact taaatacaat atctctgcaa tgcggaattc
     5941 agtggttcgt ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta
     6001 ccaccttact tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg
     6061 ctaactcatc gttaccatgt tgcaagacta caagagtatt gcataagact acatttcccc
     6121 ctccctatgc aaaagcgaaa ctactatatc ctgaggggac tcctaaccgc gtacaaccga
     6181 agccccgctt ttcgcctaaa cacaccctag tcccctcaga tacgcgtata tctggcccgt
     6241 acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca attgtcggtc
     6301 aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac ctccaacaca
     6361 caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     6421 caccatacgg atctgcgatg ataagctgtc aaacatgaga attggtcgaa attcactggc
     6481 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6541 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6601 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     6661 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     6721 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     6781 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     6841 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     6901 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     6961 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7021 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7081 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7141 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7201 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7261 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7321 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7381 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7441 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7501 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7561 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7621 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7681 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7741 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7801 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7861 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7921 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7981 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8041 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8101 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8161 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8221 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8281 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8341 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8401 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8461 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8521 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8581 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8641 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8701 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8761 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8821 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8881 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tg
//
