LOCUS       sequence-46820-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Parker JB, Palchaudhuri S, Yin H, Wei J, Chakravarti D
  TITLE     A Transcriptional Regulatory Role of the THAP11-HCF-1 Complex in 
            Colon Cancer Cell Function.
  JOURNAL   Mol Cell Biol. 2012 May;32(9):1654-70. Epub 2012 Feb 27.
  PUBMED    22371484
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2012-05"; volume: "32(9)"; pages: "1654-70. Epub 2012
            Feb 27"
COMMENT     Sequence Label: pSUPER.retro.puro-HCF-1-B
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..50
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     35..51
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(120..334)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(180..199)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        345..844
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(380..403)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     777..796
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(869..888)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tccttctcta ggcgccggaa ttagatcgat ctctcgaggt cgacggtatc gataagctta
       61 aaaaggaaga gactcaaagc aaatctcttg aatttgcttt gagtctcttc cggggatctg
      121 tggtctcata cagaacttat aagattccca aatccaaaga catttcacgt ttatggtgat
      181 ttcccagaac acatagcgac atgcaaatat tgcagggcgc cactcccctg tccctcacag
      241 ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg cctagtgaca ctgggcccgc
      301 gattccttgg agcgggttga tgacgtcagc gttcgaattc taccgggtag gggaggcgct
      361 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
      421 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
      481 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
      541 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
      601 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
      661 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
      721 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
      781 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
      841 ttcgacctgc agcccaagct agcttaccat gaccgagtac aagcccacgg
//
