LOCUS       sequence-468229-       15980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low copy number provides expression of human DNA-PKcs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15980)
  AUTHORS   Buehl CJ, Goff NJ, Hardwick SW, Gellert M, Blundell TL, Yang W, 
            Chaplin AK, Meek K
  TITLE     Two distinct long-range synaptic complexes promote different aspects
            of end processing prior to repair of DNA breaks by non-homologous 
            end joining.
  JOURNAL   Mol Cell. 2023 Mar 2;83(5):698-714.e4. doi: 
            10.1016/j.molcel.2023.01.012. Epub 2023 Jan 31.
  PUBMED    36724784
REFERENCE   2  (bases 1 to 15980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.012"; journalName: "Mol Cell"; date: 
            "2023-03-2- 2"; volume: "83"; issue: "5"; pages: "698-714.e4"
COMMENT     Sequence Label: RMCE wild type DNA-PKcs
FEATURES             Location/Qualifiers
     source          1..15980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..37)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        105..209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(210..278,279..1070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(428..447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1241..1829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1730..1749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1983..2000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2065..2087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2084..2100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2084..2100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2134..2167
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2311..2614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(15554..15571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15560..15784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(15822..15855)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(15888..15904)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15937..15953)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15946..15968)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
       61 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      421 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
      481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
      721 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
      781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
      841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
      901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
      961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1381 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1981 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     2041 atctgtgcgg tatttcacac cgcaagcgga taacaatttc acacaggaaa cagctatgac
     2101 catgattacg ccaagcttgc atgcctgcag gggataactt cgtataatgt atgctatacg
     2161 aagttatggg gccgctctag cctcgaggcc gtaatgagac gcacaaacta atatcacaaa
     2221 ctggaaatgt ctatcaatat atagttgctc tagttattaa tagtaatcaa ttacggggtc
     2281 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2341 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2401 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2461 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2521 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2581 gtacatctac gtattagtca tcgctattac catgcatggt cgaggtgagc cccacgttct
     2641 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     2701 aattattttg tgcagcgatg ggggcggggg gggcacgcgg gtgcgcgtgg agcgcgcagg
     2761 gagggggcgc cggggggggg gggggggggg ggcggggggg gggggggggg gggggggggg
     2821 gggggggggg gggggggggg gggggggggg cgagggggag aggtgcggcg gcagccaatc
     2881 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     2941 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
     3001 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     3061 gcgggcggga cggcccttct ccttcgggct gtaattagca tcacgcgtgg tttttccgct
     3121 agccatggcg ggctccggag ccggtgtgcg ttgctccctg ctgcggctgc aggagacctt
     3181 gtccgctgcg gaccgctgcg gtgctgccct ggccggtcat caactgatcc gcggcctggg
     3241 gcaggaatgc gtcctgagca gcagccccgc ggtgctggca ttacagacat ctttagtttt
     3301 ttccagagat ttcggtttgc ttgtatttgt ccggaagtca ctcaacagta ttgaatttcg
     3361 tgaatgtaga gaagaaatcc taaagttttt atgtattttc ttagaaaaaa tgggccagaa
     3421 gatcgcacct tactctgttg aaattaagaa cacttgtacc agtgtttata caaaagatag
     3481 agctgctaaa tgtaaaattc cagccctgga ccttcttatt aagttacttc agacttttag
     3541 aagttctaga ctcatggatg aatttaaaat tggagaatta tttagtaaat tctatggaga
     3601 acttgcattg aaaaaaaaaa taccagatac agttttagaa aaagtatatg agctcctagg
     3661 attattgggt gaagttcatc ctagtgagat gataaataat gcagaaaacc tgttccgcgc
     3721 ttttctgggt gaacttaaga cccagatgac atcagcagta agagagccca aactacctgt
     3781 tctggcagga tgtctgaagg ggttgtcctc acttctgtgc aacttcacta agtccatgga
     3841 agaagatccc cagacttcaa gggagatttt taattttgta ctaaaggcaa ttcgtcctca
     3901 gattgatctg aagagatatg ctgtgccctc agctggcttg cgcctatttg ccctgcatgc
     3961 atctcagttt agcacctgcc ttctggacaa ctacgtgtct ctatttgaag tcttgttaaa
     4021 gtggtgtgcc cacacaaatg tagaattgaa aaaagctgca ctttcagccc tggaatcctt
     4081 tctgaaacag gtttctaata tggtggcgaa aaatgcagaa atgcataaaa ataaactgca
     4141 gtactttatg gagcagtttt atggaatcat cagaaatgtg gattcgaaca acaaggagtt
     4201 atctattgct atccgtggat atggactttt tgcaggaccg tgcaaggtta taaacgcaaa
     4261 agatgttgac ttcatgtacg ttgagctcat tcagcgctgc aagcagatgt tcctcaccca
     4321 gacagacact ggtgacgacc gtgtttatca gatgccaagc ttcctccagt ctgttgcaag
     4381 cgtcttgctg taccttgaca cagttcctga ggtgtatact ccagttctgg agcacctcgt
     4441 ggtgatgcag atagacagtt tcccacagta cagtccaaaa atgcagctgg tgtgttgcag
     4501 agccatagtg aaggtgttcc tagctttggc agcaaaaggg ccagttctca ggaattgcat
     4561 tagtactgtg gtgcatcagg gtttaatcag aatatgttct aaaccagtgg tccttccaaa
     4621 gggacctgag tctgaatctg aagaccaccg tgcttcaggg gaagtcagaa ctggcaaatg
     4681 gaaggtgccc acatacaaag actacgtgga tctcttcaga catctcctga gctctgacca
     4741 gatgatggat tctattttag cagatgaagc atttttctct gtgaattcct ccagtgaaag
     4801 tctgaatcat ttactttatg atgaatttgt aaaatccgtt ttgaagattg ttgagaaatt
     4861 ggatcttaca cttgaaatac agactgttgg ggaacaagag aatggagatg aggcacctgg
     4921 tgtttggatg atcccaactt cagatccagc ggctaacttg catccagcta aacctaaaga
     4981 tttttcggct ttcattaacc tggtggaatt ttgcagagag attctccctg agaaacaagc
     5041 agaatttttt gaaccatggg tgtactcatt ttcatatgaa ttaattttgc aatctacaag
     5101 gttgcccctc atcagtggtt tctacaaatt gctttctatt acagtaagaa atgccaagaa
     5161 aataaaatat ttcgagggag ttagtccaaa gagtctgaaa cactctcctg aagacccaga
     5221 aaagtattct tgctttgctt tatttgtgaa atttggcaaa gaggtggcag ttaaaatgaa
     5281 gcagtacaaa gatgaacttt tggcctcttg tttgaccttt cttctgtcct tgccacacaa
     5341 catcattgaa ctcgatgtta gagcctacgt tcctgcactg cagatggctt tcaaactggg
     5401 cctgagctat acccccttgg cagaagtagg actgaatgct ctagaagaat ggtcaattta
     5461 tattgacaga catgtaatgc agccttatta caaagacatt ctcccctgcc tggatggata
     5521 cctgaagact tcagccttgt cagatgagac caagaataac tgggaagtgt cagctctttc
     5581 tcgggctgcc cagaaaggat ttaataaagt ggtgttaaag catctgaaga agacaaagaa
     5641 cctttcatca aacgaagcaa tatccttaga agaaataaga attagagtag tacaaatgct
     5701 tggatctcta ggaggacaaa taaacaaaaa tcttctgaca gtcacgtcct cagatgagat
     5761 gatgaagagc tatgtggcct gggacagaga gaagcggctg agctttgcag tgccctttag
     5821 agagatgaaa cctgtcattt tcctggatgt gttcctgcct cgagtcacag aattagcgct
     5881 cacagccagt gacagacaaa ctaaggttgc agcctgtgaa cttttacata gcatggttat
     5941 gtttatgttg ggcaaagcca cgcagatgcc agaaggggga cagggagccc cacccatgta
     6001 ccagctctat aagcggacgt ttcctgtgct gcttcgactt gcgtgtgatg ttgatcaggt
     6061 gacaaggcaa ctgtatgagc cactagttat gcagctgatt cactggttca ctaacaacaa
     6121 gaaatttgaa agtcaggata ctgttgcctt actagaagct atattggatg gaattgtgga
     6181 ccctgttgac agtactttaa gagatttttg tggtcggtgt attcgagaat tccttaaatg
     6241 gtccattaag caaataacac cacagcagca ggagaagagt ccagtaaaca ccaaatcgct
     6301 tttcaagcga ctttatagcc ttgcgcttca ccccaatgct ttcaagaggc tgggagcatc
     6361 acttgccttt aataatatct acagggaatt cagggaagaa gagtctctgg tggaacagtt
     6421 tgtgtttgaa gccttggtga tatacatgga gagtctggcc ttagcacatg cagatgagaa
     6481 gtccttaggt acaattcaac agtgttgtga tgccattgat cacctatgcc gcatcattga
     6541 aaagaagcat gtttctttaa ataaagcaaa gaaacgacgt ttgccgcgag gatttccacc
     6601 ttccgcatca ttgtgtttat tggatctggt caagtggctt ttagctcatt gtgggaggcc
     6661 ccagacagaa tgtcgacaca aatccattga actcttttat aaattcgttc ctttattgcc
     6721 aggcaacaga tcccctaatt tgtggctgaa agatgttctc aaggaagaag gtgtctcttt
     6781 tctcatcaac acctttgagg ggggtggctg tggccagccc tcgggcatcc tggcccagcc
     6841 caccctcttg taccttcggg ggccattcag cctgcaggcc acgctatgct ggctggacct
     6901 gctcctggcc gcgttggagt gctacaacac gttcattggc gagagaactg taggagcgct
     6961 ccaggtccta ggtactgaag cccagtcttc acttttgaaa gcagtggctt tcttcttaga
     7021 aagcattgcc atgcatgaca ttatagcagc agaaaagtgc tttggcactg gggcagcagg
     7081 taacagaaca agcccacaag agggagaaag gtacaactac agcaaatgca ccgttgtggt
     7141 ccggattatg gagtttacca cgactctgct aaacacctcc ccggaaggat ggaagctcct
     7201 gaagaaggac ttgtgtaata cacacctgat gagagtcctg gtgcagacgc tgtgtgagcc
     7261 cgcaagcata ggtttcaaca tcggagacgt ccaggttatg gctcatcttc ctgatgtttg
     7321 tgtgaatctg atgaaagctc taaagatgtc cccatacaaa gatatcctag agacccatct
     7381 gagagagaaa ataacagcac agagcattga ggagctttgt gccgtcaact tgtatggccc
     7441 tgacgcgcaa gtggacagga gcaggctggc tgctgttgtg tctgcctgta aacagcttca
     7501 cagagctggg cttctgcata atatattacc gtctcagtcc acagatttgc atcattctgt
     7561 tggcacagaa cttctttccc tggtttataa aggcattgcc cctggagatg agagacagtg
     7621 tctgccttct ctagacctca gttgtaagca gctggccagc ggacttctgg agttagcctt
     7681 tgcttttgga ggactgtgtg agcgccttgt gagtcttctc ctgaacccag cggtgctgtc
     7741 cacggcgtcc ttgggcagct cacagggcag cgtcatccac ttctcccatg gggagtattt
     7801 ctatagcttg ttctcagaaa cgatcaacac ggaattattg aaaaatctgg atcttgctgt
     7861 attggagctc atgcagtctt cagtggataa taccaaaatg gtgagtgccg ttttgaacgg
     7921 catgttagac cagagcttca gggagcgagc aaaccagaaa caccaaggac tgaaacttgc
     7981 gactacaatt ctgcaacact ggaagaagtg tgattcatgg tgggccaaag attcccctct
     8041 cgaaactaaa atggcagtgc tggccttact ggcaaaaatt ttacagattg attcatctgt
     8101 atcttttaat acaagtcatg gttcattccc tgaagtcttt acaacatata ttagtctact
     8161 tgctgacaca aagctggatc tacatttaaa gggccaagct gtcactcttc ttccattctt
     8221 caccagcctc actggaggca gtctggagga acttagacgt gttctggagc agctcatcgt
     8281 tgctcacttc cccatgcagt ccagggaatt tcctccagga actccgcggt tcaataatta
     8341 tgtggactgc atgaaaaagt ttctagatgc attggaatta tctcaaagcc ctatgttgtt
     8401 ggaattgatg acagaagttc tttgtcggga acagcagcat gtcatggaag aattatttca
     8461 atccagtttc aggaggattg ccagaagggg ttcatgtgtc acacaagtag gccttctgga
     8521 aagcgtgtat gaaatgttca ggaaggatga cccccgccta agtttcacac gccagtcctt
     8581 tgtggaccgc tccctcctca ctctgctgtg gcactgtagc ctggatgctt tgagagaatt
     8641 cttcagcaca attgtggtgg atgccattga tgtgttgaag tccaggttta caaagctaaa
     8701 tgaatctacc tttgatactc aaatcaccaa gaagatgggc tactataaga ttctagacgt
     8761 gatgtattct cgccttccca aagatgatgt tcatgctaag gaatcaaaaa ttaatcaagt
     8821 tttccatggc tcgtgtatta cagaaggaaa tgaacttaca aagacattga ttaaattgtg
     8881 ctacgatgca tttacagaga acatggcagg agagaatcag ctgctggaga ggagaagact
     8941 ttaccattgt gcagcataca actgcgccat atctgtcatc tgctgtgtct tcaatgagtt
     9001 aaaattttac caaggttttc tgtttagtga aaaaccagaa aagaacttgc ttatttttga
     9061 aaatctgatc gacctgaagc gccgctataa ttttcctgta gaagttgagg ttcctatgga
     9121 aagaaagaaa aagtacattg aaattaggaa agaagccaga gaagcagcaa atggggattc
     9181 agatggtcct tcctatatgt cttccctgtc atatttggca gacagtaccc tgagtgagga
     9241 aatgagtcaa tttgatttct caaccggagt tcagagctat tcatacagct cccaagaccc
     9301 tagacctgcc actggtcgtt ttcggagacg ggagcagcgg gaccccacgg tgcatgatga
     9361 tgtgctggag ctggagatgg acgagctcaa tcggcatgag tgcatggcgc ccctgacggc
     9421 cctggtcaag cacatgcaca gaagcctggg cccgcctcaa ggagaagagg attcagtgcc
     9481 aagagatctt ccttcttgga tgaaattcct ccatggcaaa ctgggaaatc caatagtacc
     9541 attaaatatc cgtctcttct tagccaagct tgttattaat acagaagagg tctttcgccc
     9601 ttacgcgaag cactggctta gccccttgct gcagctggct gcttctgaaa acaatggagg
     9661 agaaggaatt cactacatgg tggttgagat agtggccact attctttcat ggacaggctt
     9721 ggccactcca acaggggtcc ctaaagatga agtgttagca aatcgattgc ttaatttcct
     9781 aatgaaacat gtctttcatc caaaaagagc tgtgtttaga cacaaccttg aaattataaa
     9841 gacccttgtc gagtgctgga aggattgttt atccatccct tataggttaa tatttgaaaa
     9901 gttttccggt aaagatccta attctaaaga caactcagta gggattcaat tgctaggcat
     9961 cgtgatggcc aatgacctgc ctccctatga cccacagtgt ggcatccaga gtagcgaata
    10021 cttccaggct ttggtgaata atatgtcctt tgtaagatat aaagaagtgt atgccgctgc
    10081 agcagaagtt ctaggactta tacttcgata tgttatagag agaaaaaaca tactggagga
    10141 gtctctgtgt gaactggttg cgaaacaatt gaagcaacat cagaatacta tggaggacaa
    10201 gtttattgtg tgcttgaaca aagtgaccaa gagcttccct cctcttgcag acaggttcat
    10261 gaatgctgtg ttctttctgc tgccaaaatt tcatggagtg ttgaaaacac tctgtctgga
    10321 ggtggtactt tgtcgtgtgg agggaatgac agagctgtac ttccagttaa agagcaagga
    10381 cttcgttcaa gtcatgagac atagagatga tgaaagacaa aaagtatgtt tggacataat
    10441 ttataagatg atgccaaagt taaaaccagt agaactccga gaacttctga accccgttgt
    10501 ggaattcgtt tcccatcctt ctacaacatg tagggaacaa atgtataata ttctcatgtg
    10561 gattcatgat aattacagag atccagaaag tgagacagat aatgactccc aggaaatatt
    10621 taagttggca aaagatgtgc tgattcaagg attgatcgat gagaaccctg gacttcaatt
    10681 aattattcga aatttctgga gccatgaaac taggttacct tcaaatacct tggaccggtt
    10741 gctggcacta aattccttat attctcctaa gatagaagtg cactttttaa gtttagcaac
    10801 aaattttctg ctcgaaatga ccagcatgag cccagattat ccaaacccca tgttcgagca
    10861 tcctctgtca gaatgcgaat ttcaggaata taccattgat tctgattggc gtttccgaag
    10921 tactgttctc actccgatgt ttgtggagac ccaggcctcc cagggcactc tccaggcccg
    10981 tacccaggaa gggtccctct cagctcgctg gccagtggca gggcagataa gggccaccca
    11041 gcagcagcat gacttcacac tgacacagac tgcagatgga agaagctcat ttgattggct
    11101 gaccgggagc agcactgacc cgctggtcga ccacaccagt ccctcatctg actccttgct
    11161 gtttgcccac aagaggagtg aaaggttaca gagagcaccc ttgaagtcag tggggcctga
    11221 ttttgggaaa aaaaggctgg gccttccagg ggacgaggtg gataacaaag tgaaaggtgc
    11281 ggccggccgg acggacctac tacgactgcg cagacggttt atgagggacc aggagaagct
    11341 tagtttgatg tatgcccgaa aaggcgttgc tgagcaaaaa cgagagaagg aaatcaagag
    11401 tgagttaaaa atgaagcagg atgcccaggt cgttctgtac agaagctacc ggcacggaga
    11461 ccttcctgac attcagatca agcacagcag cctcatcacc ccgttacagg ccgtggccca
    11521 gagggaccca ataatcgcga aacagctctt tagcagcttg ttttctggaa ttttgaaaga
    11581 gatggataaa tttaagacac tgtctgaaaa aaacaacatc actcaaaagt tgcttcaaga
    11641 cttcaatcgt tttcttaata ccaccttctc tttctttcca ccctttgtct cttgtattca
    11701 ggacattagc tgtcagcacg cagccctgct gagcctcgac ccagcggctg ttagcgctgg
    11761 ttgcctggcc agcctacagc agcctgttgg catccgccta ctagaggagg ctattctttg
    11821 tctgctgcct gctgagctgc ccgccaagcg agtccgtggg aaggctagac ctcctcctga
    11881 tgtcctcaga tgggtggagc ttgctaagct gtatagatca attggagaat acgacgtcct
    11941 ccgtgggatt tttaccagtg agataggaac aaagcaaatc actcagagtg cattattagc
    12001 agaagccaga agtgattatt ctgaagctgc taagcagtat gatgaggctc tcaataaaca
    12061 agactgggta gatggtgagc ccacagaagc cgagaaggat ttttgggaac ttgcatccct
    12121 tgactgttac aaccaccttg ctgagtggaa atcacttgaa tactgttcta cagccagtat
    12181 agacagtgag aaccccccag acctaaataa aatctggagt gaaccatttt atcaggaaac
    12241 atatctacct tacatgatcc gcagcaagct gaagctgctg ctccagggag aggctgacca
    12301 gtccctgctg acatttattg acaaagctat gcacggggag ctccagaagg cgattctaga
    12361 gcttcattac agtcaagagc tgagtctgct ttacctcctg caagatgatg ttgacagagc
    12421 caaatattac attcaaaatg gcattcagag ttttatgcag aattattcta gtattgatgt
    12481 cctcttacac caaagtagac tcaccaaatt gcagtctgta caggctttaa cagaaattca
    12541 ggagttcatc agctttataa gcaaacaagg caatttatca tctcaagttc cccttaagag
    12601 acttctgaac acctgggcaa acagatatcc agatgctaaa atggacccaa tgaacatctg
    12661 ggatgacatc atcacaaatc gatgtttctt tctcagcaaa atagaggaga agcttacccc
    12721 tcttccagaa gataatagta tgaatgtgga tcaagatgga gaccccagtg acaggatgga
    12781 agtgcaagag caggaagaag atatcagctc cctgatcagg agttgcaagt tttccatgaa
    12841 aatgaagatg atagacagtg cccggaagca gaacaatttc tcacttgcta tgaaactact
    12901 gaaggagctg cataaagagt caaaaaccag agacgattgg ctggtgagct gggtgcagag
    12961 ctactgccgc ctgagccact gccggagccg gtcccagggc tgctctgagc aggtgctcac
    13021 tgtgctgaaa acagtctctt tgttggatga gaacaacgtg tcaagctact taagcaaaaa
    13081 tattctggct ttccgtgacc agaacattct cttgggtaca acttacagga tcatagcgaa
    13141 tgctctcagc agtgagccag cctgccttgc tgaaatcgag gaggacaagg ctagaagaat
    13201 cttagagctt tctggatcca gttcagagga ttcagagaag gtgatcgcgg gtctgtacca
    13261 gagagcattc cagcacctct ctgaggctgt gcaggcggct gaggaggagg cccagcctcc
    13321 ctcctggagc tgtgggcctg cagctggggt gattgatgct tacatgacgc tggcagattt
    13381 ctgtgaccaa cagctgcgca aggaggaaga gaatgcatca gttactgatt ctgcagaact
    13441 gcaggcgtat ccagcacttg tggtggagaa aatgttgaaa gctttaaaat taaattccaa
    13501 tgaagccaga ttgaagtttc ctagattact tcagattata gaacggtatc cagaggagac
    13561 tttgagcctc atgacaaaag agatctcttc cgttccctgc tggcagttca tcagctggat
    13621 cagccacatg gtggccttac tggacaaaga ccaagccgtt gctgttcagc actctgtgga
    13681 agaaatcact gataactacc cgcaggctat tgtttatccc ttcatcataa gcagcgaaag
    13741 ctattccttc aaggatactt ctactggtca taagaataag gagtttgtgg caaggattaa
    13801 aagtaagttg gatcaaggag gagtgattca agattttatt aatgccttag atcagctctc
    13861 taatcctgaa ctgctcttta aggattggag caatgatgta agagctgaac tagcaaaaac
    13921 ccctgtaaat aaaaaaaaca ttgaaaaaat gtatgaaaga atgtatgcag ccttgggtga
    13981 cccaaaggct ccaggcctgg gggcctttag aaggaagttt attcagactt ttggaaaaga
    14041 atttgataaa cattttggga aaggaggttc taaactactg agaatgaagc tcagtgactt
    14101 caacgacatt accaacatgc tacttttaaa aatgaacaaa gactcaaagc cccctgggaa
    14161 tctgaaagaa tgttcaccct ggatgagcga cttcaaagtg gagttcctga gaaatgagct
    14221 ggagattccc ggtcagtatg acggtagggg aaagccattg ccagagtacc acgtgcgaat
    14281 cgccgggttt gatgagcggg tgacagtcat ggcgtctctg cgaaggccca agcgcatcat
    14341 catccgtggc catgacgaga gggaacaccc tttcctggtg aagggtggcg aggacctgcg
    14401 gcaggaccag cgcgtggagc agctcttcca ggtcatgaat gggatcctgg cccaagactc
    14461 cgcctgcagc cagagggccc tgcagctgag gacctatagc gttgtgccca tgacctccag
    14521 gttaggatta attgagtggc ttgaaaatac tgttaccttg aaggaccttc ttttgaacac
    14581 catgtcccaa gaggagaagg cggcttacct gagtgatccc agggcaccgc cgtgtgaata
    14641 taaagattgg ctgacaaaaa tgtcaggaaa acatgatgtt ggagcttaca tgctaatgta
    14701 taagggcgct aatcgtactg aaacagtcac gtcttttaga aaacgagaaa gtaaagtgcc
    14761 tgctgatctc ttaaagcggg ccttcgtgag gatgagtaca agccctgagg ctttcctggc
    14821 gctccgctcc cacttcgcca gctctcacgc tctgatatgc atcagccact ggatcctcgg
    14881 gattggagac agacatctga acaactttat ggtggccatg gagactggtg gcgtgatcgg
    14941 gatcgacttt gggcatgcgt ttggatccgc tacacagttt ctgccagtcc ctgagttgat
    15001 gccttttcgg ctaactcgcc agtttatcaa tctgatgtta ccaatgaaag aaacgggcct
    15061 tatgtacagc atcatggtac acgcactccg ggccttccgc tcagaccctg gcctgctcac
    15121 caacaccatg gatgtgtttg tcaaggagcc ctcctttgat tggaaaaatt ttgaacagaa
    15181 aatgctgaaa aaaggagggt catggattca agaaataaat gttgctgaaa aaaattggta
    15241 cccccgacag aaaatatgtt acgctaagag aaagttagca ggtgccaatc cagcagtcat
    15301 tacttgtgat gagctactcc tgggtcatga gaaggcccct gccttcagag actatgtggc
    15361 tgtggcacga ggaagcaaag atcacaacat tcgtgcccaa gaaccagaga gtgggctttc
    15421 agaagagact caagtgaagt gcctgatgga ccaggcaaca gaccccaaca tccttggcag
    15481 aacctgggaa ggatgggagc cctggatgtg aggtctgcgg ccgcgatctg tcgacgataa
    15541 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
    15601 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
    15661 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
    15721 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
    15781 atggcttctg aggcggaaag aaccagatcg gatccatcga tataacttcg tataggagac
    15841 tttatacgaa gttatctcga tgaaggggtt ctctagatcg agaattcact ggccgtcgtt
    15901 ttacaaggcg cgcccccggg taccgagctc gaattcactg gccgtcgttt tacaacgtcg
    15961 tgactgggaa aaccctggcg
//
