LOCUS       sequence-468243-       16977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low copy number provides expression of human DNA-PKcs with an 
            N-terminal halo-flag tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16977)
  AUTHORS   Buehl CJ, Goff NJ, Hardwick SW, Gellert M, Blundell TL, Yang W, 
            Chaplin AK, Meek K
  TITLE     Two distinct long-range synaptic complexes promote different aspects
            of end processing prior to repair of DNA breaks by non-homologous 
            end joining.
  JOURNAL   Mol Cell. 2023 Mar 2;83(5):698-714.e4. doi: 
            10.1016/j.molcel.2023.01.012. Epub 2023 Jan 31.
  PUBMED    36724784
REFERENCE   2  (bases 1 to 16977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.012"; journalName: "Mol Cell"; date: 
            "2023-03-2- 2"; volume: "83"; issue: "5"; pages: "698-714.e4"
COMMENT     Sequence Label: RMCE halo-flag DNA-PKcs
FEATURES             Location/Qualifiers
     source          1..16977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..37)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        105..209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(210..278,279..1070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(428..447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1241..1829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1730..1749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1983..2000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2065..2087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2084..2100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2084..2100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2134..2167
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2311..2614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             3066..3131
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    3132..3165
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3174..4064
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             4077..4097
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(16551..16568)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    16557..16781
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(16819..16852)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(16885..16901)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16934..16950)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16943..16965)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
       61 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      421 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
      481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
      721 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
      781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
      841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
      901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
      961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1381 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1981 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     2041 atctgtgcgg tatttcacac cgcaagcgga taacaatttc acacaggaaa cagctatgac
     2101 catgattacg ccaagcttgc atgcctgcag gggataactt cgtataatgt atgctatacg
     2161 aagttatggg gccgctctag cctcgaggcc gtaatgagac gcacaaacta atatcacaaa
     2221 ctggaaatgt ctatcaatat atagttgctc tagttattaa tagtaatcaa ttacggggtc
     2281 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2341 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2401 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2461 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2521 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2581 gtacatctac gtattagtca tcgctattac catgcatggt cgaggtgagc cccacgttct
     2641 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     2701 aattattttg tgcagcgatg ggggcggggg gggggggggg cgcgcgccag gggggggggg
     2761 cggggggggg gggggggggg gggggggggg gggggggggg ggggggcgga gaggggcggc
     2821 ggcagccaat cagggcggcg cgctcggaaa gtttcctttt agggcggggg ggcggcggcg
     2881 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
     2941 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
     3001 cccacaggtg agcgggcggg acggcccttc tccttcgggc tgtaattagc atcacgcgta
     3061 ccatggacta caaagaccat gacggtgatt ataaagatca tgatatcgat tacaaggatg
     3121 acgatgacaa gataacttcg tataatgtat gctatacgaa gttatccggt accatggcag
     3181 aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc gagcgcatgc
     3241 actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac ggtaacccga
     3301 cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat cgctgcattg
     3361 ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat ttcttcgacg
     3421 accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag gtcgtcctgg
     3481 tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat ccagagcgcg
     3541 tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac gaatggccag
     3601 aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc aagctgatca
     3661 tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg
     3721 aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt
     3781 ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg
     3841 aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc tggggcaccc
     3901 caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg cctaactgca
     3961 aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg gacctgatcg
     4021 gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc cggcgagcca accactgagg
     4081 atctgtactt tcagagcgat aacgcgatcg cttccggtac catggcagga agtggagcag
     4141 gtgtgagatg cagtctacta agactacaag aaaccttaag tgctgcggac cgctgcggtg
     4201 ctgccctggc cggtcatcaa ctgatccgcg gcctggggca ggaatgcgtc ctgagcagca
     4261 gccccgcggt gctggcatta cagacatctt tagttttttc cagagatttc ggtttgcttg
     4321 tatttgtccg gaagtcactc aacagtattg aatttcgtga atgtagagaa gaaatcctaa
     4381 agtttttatg tattttctta gaaaaaatgg gccagaagat cgcaccttac tctgttgaaa
     4441 ttaagaacac ttgtaccagt gtttatacaa aagatagagc tgctaaatgt aaaattccag
     4501 ccctggacct tcttattaag ttacttcaga cttttagaag ttctagactc atggatgaat
     4561 ttaaaattgg agaattattt agtaaattct atggagaact tgcattgaaa aaaaaaatac
     4621 cagatacagt tttagaaaaa gtatatgagc tcctaggatt attgggtgaa gttcatccta
     4681 gtgagatgat aaataatgca gaaaacctgt tccgcgcttt tctgggtgaa cttaagaccc
     4741 agatgacatc agcagtaaga gagcccaaac tacctgttct ggcaggatgt ctgaaggggt
     4801 tgtcctcact tctgtgcaac ttcactaagt ccatggaaga agatccccag acttcaaggg
     4861 agatttttaa ttttgtacta aaggcaattc gtcctcagat tgatctgaag agatatgctg
     4921 tgccctcagc tggcttgcgc ctatttgccc tgcatgcatc tcagtttagc acctgccttc
     4981 tggacaacta cgtgtctcta tttgaagtct tgttaaagtg gtgtgcccac acaaatgtag
     5041 aattgaaaaa agctgcactt tcagccctgg aatcctttct gaaacaggtt tctaatatgg
     5101 tggcgaaaaa tgcagaaatg cataaaaata aactgcagta ctttatggag cagttttatg
     5161 gaatcatcag aaatgtggat tcgaacaaca aggagttatc tattgctatc cgtggatatg
     5221 gactttttgc aggaccgtgc aaggttataa acgcaaaaga tgttgacttc atgtacgttg
     5281 agctcattca gcgctgcaag cagatgttcc tcacccagac agacactggt gacgaccgtg
     5341 tttatcagat gccaagcttc ctccagtctg ttgcaagcgt cttgctgtac cttgacacag
     5401 ttcctgaggt gtatactcca gttctggagc acctcgtggt gatgcagata gacagtttcc
     5461 cacagtacag tccaaaaatg cagctggtgt gttgcagagc catagtgaag gtgttcctag
     5521 ctttggcagc aaaagggcca gttctcagga attgcattag tactgtggtg catcagggtt
     5581 taatcagaat atgttctaaa ccagtggtcc ttccaaaggg acctgagtct gaatctgaag
     5641 accaccgtgc ttcaggggaa gtcagaactg gcaaatggaa ggtgcccaca tacaaagact
     5701 acgtggatct cttcagacat ctcctgagct ctgaccagat gatggattct attttagcag
     5761 atgaagcatt tttctctgtg aattcctcca gtgaaagtct gaatcattta ctttatgatg
     5821 aatttgtaaa atccgttttg aagattgttg agaaattgga tcttacactt gaaatacaga
     5881 ctgttgggga acaagagaat ggagatgagg cacctggtgt ttggatgatc ccaacttcag
     5941 atccagcggc taacttgcat ccagctaaac ctaaagattt ttcggctttc attaacctgg
     6001 tggaattttg cagagagatt ctccctgaga aacaagcaga attttttgaa ccatgggtgt
     6061 actcattttc atatgaatta attttgcaat ctacaaggtt gcccctcatc agtggtttct
     6121 acaaattgct ttctattaca gtaagaaatg ccaagaaaat aaaatatttc gagggagtta
     6181 gtccaaagag tctgaaacac tctcctgaag acccagaaaa gtattcttgc tttgctttat
     6241 ttgtgaaatt tggcaaagag gtggcagtta aaatgaagca gtacaaagat gaacttttgg
     6301 cctcttgttt gacctttctt ctgtccttgc cacacaacat cattgaactc gatgttagag
     6361 cctacgttcc tgcactgcag atggctttca aactgggcct gagctatacc cccttggcag
     6421 aagtaggact gaatgctcta gaagaatggt caatttatat tgacagacat gtaatgcagc
     6481 cttattacaa agacattctc ccctgcctgg atggatacct gaagacttca gccttgtcag
     6541 atgagaccaa gaataactgg gaagtgtcag ctctttctcg ggctgcccag aaaggattta
     6601 ataaagtggt gttaaagcat ctgaagaaga caaagaacct ttcatcaaac gaagcaatat
     6661 ccttagaaga aataagaatt agagtagtac aaatgcttgg atctctagga ggacaaataa
     6721 acaaaaatct tctgacagtc acgtcctcag atgagatgat gaagagctat gtggcctggg
     6781 acagagagaa gcggctgagc tttgcagtgc cctttagaga gatgaaacct gtcattttcc
     6841 tggatgtgtt cctgcctcga gtcacagaat tagcgctcac agccagtgac agacaaacta
     6901 aggttgcagc ctgtgaactt ttacatagca tggttatgtt tatgttgggc aaagccacgc
     6961 agatgccaga agggggacag ggagccccac ccatgtacca gctctataag cggacgtttc
     7021 ctgtgctgct tcgacttgcg tgtgatgttg atcaggtgac aaggcaactg tatgagccac
     7081 tagttatgca gctgattcac tggttcacta acaacaagaa atttgaaagt caggatactg
     7141 ttgccttact agaagctata ttggatggaa ttgtggaccc tgttgacagt actttaagag
     7201 atttttgtgg tcggtgtatt cgagaattcc ttaaatggtc cattaagcaa ataacaccac
     7261 agcagcagga gaagagtcca gtaaacacca aatcgctttt caagcgactt tatagccttg
     7321 cgcttcaccc caatgctttc aagaggctgg gagcatcact tgcctttaat aatatctaca
     7381 gggaattcag ggaagaagag tctctggtgg aacagtttgt gtttgaagcc ttggtgatat
     7441 acatggagag tctggcctta gcacatgcag atgagaagtc cttaggtaca attcaacagt
     7501 gttgtgatgc cattgatcac ctatgccgca tcattgaaaa gaagcatgtt tctttaaata
     7561 aagcaaagaa acgacgtttg ccgcgaggat ttccaccttc cgcatcattg tgtttattgg
     7621 atctggtcaa gtggctttta gctcattgtg ggaggcccca gacagaatgt cgacacaaat
     7681 ccattgaact cttttataaa ttcgttcctt tattgccagg caacagatcc cctaatttgt
     7741 ggctgaaaga tgttctcaag gaagaaggtg tctcttttct catcaacacc tttgaggggg
     7801 gtggctgtgg ccagccctcg ggcatcctgg cccagcccac cctcttgtac cttcgggggc
     7861 cattcagcct gcaggccacg ctatgctggc tggacctgct cctggccgcg ttggagtgct
     7921 acaacacgtt cattggcgag agaactgtag gagcgctcca ggtcctaggt actgaagccc
     7981 agtcttcact tttgaaagca gtggctttct tcttagaaag cattgccatg catgacatta
     8041 tagcagcaga aaagtgcttt ggcactgggg cagcaggtaa cagaacaagc ccacaagagg
     8101 gagaaaggta caactacagc aaatgcaccg ttgtggtccg gattatggag tttaccacga
     8161 ctctgctaaa cacctccccg gaaggatgga agctcctgaa gaaggacttg tgtaatacac
     8221 acctgatgag agtcctggtg cagacgctgt gtgagcccgc aagcataggt ttcaacatcg
     8281 gagacgtcca ggttatggct catcttcctg atgtttgtgt gaatctgatg aaagctctaa
     8341 agatgtcccc atacaaagat atcctagaga cccatctgag agagaaaata acagcacaga
     8401 gcattgagga gctttgtgcc gtcaacttgt atggccctga cgcgcaagtg gacaggagca
     8461 ggctggctgc tgttgtgtct gcctgtaaac agcttcacag agctgggctt ctgcataata
     8521 tattaccgtc tcagtccaca gatttgcatc attctgttgg cacagaactt ctttccctgg
     8581 tttataaagg cattgcccct ggagatgaga gacagtgtct gccttctcta gacctcagtt
     8641 gtaagcagct ggccagcgga cttctggagt tagcctttgc ttttggagga ctgtgtgagc
     8701 gccttgtgag tcttctcctg aacccagcgg tgctgtccac ggcgtccttg ggcagctcac
     8761 agggcagcgt catccacttc tcccatgggg agtatttcta tagcttgttc tcagaaacga
     8821 tcaacacgga attattgaaa aatctggatc ttgctgtatt ggagctcatg cagtcttcag
     8881 tggataatac caaaatggtg agtgccgttt tgaacggcat gttagaccag agcttcaggg
     8941 agcgagcaaa ccagaaacac caaggactga aacttgcgac tacaattctg caacactgga
     9001 agaagtgtga ttcatggtgg gccaaagatt cccctctcga aactaaaatg gcagtgctgg
     9061 ccttactggc aaaaatttta cagattgatt catctgtatc ttttaataca agtcatggtt
     9121 cattccctga agtctttaca acatatatta gtctacttgc tgacacaaag ctggatctac
     9181 atttaaaggg ccaagctgtc actcttcttc cattcttcac cagcctcact ggaggcagtc
     9241 tggaggaact tagacgtgtt ctggagcagc tcatcgttgc tcacttcccc atgcagtcca
     9301 gggaatttcc tccaggaact ccgcggttca ataattatgt ggactgcatg aaaaagtttc
     9361 tagatgcatt ggaattatct caaagcccta tgttgttgga attgatgaca gaagttcttt
     9421 gtcgggaaca gcagcatgtc atggaagaat tatttcaatc cagtttcagg aggattgcca
     9481 gaaggggttc atgtgtcaca caagtaggcc ttctggaaag cgtgtatgaa atgttcagga
     9541 aggatgaccc ccgcctaagt ttcacacgcc agtcctttgt ggaccgctcc ctcctcactc
     9601 tgctgtggca ctgtagcctg gatgctttga gagaattctt cagcacaatt gtggtggatg
     9661 ccattgatgt gttgaagtcc aggtttacaa agctaaatga atctaccttt gatactcaaa
     9721 tcaccaagaa gatgggctac tataagattc tagacgtgat gtattctcgc cttcccaaag
     9781 atgatgttca tgctaaggaa tcaaaaatta atcaagtttt ccatggctcg tgtattacag
     9841 aaggaaatga acttacaaag acattgatta aattgtgcta cgatgcattt acagagaaca
     9901 tggcaggaga gaatcagctg ctggagagga gaagacttta ccattgtgca gcatacaact
     9961 gcgccatatc tgtcatctgc tgtgtcttca atgagttaaa attttaccaa ggttttctgt
    10021 ttagtgaaaa accagaaaag aacttgctta tttttgaaaa tctgatcgac ctgaagcgcc
    10081 gctataattt tcctgtagaa gttgaggttc ctatggaaag aaagaaaaag tacattgaaa
    10141 ttaggaaaga agccagagaa gcagcaaatg gggattcaga tggtccttcc tatatgtctt
    10201 ccctgtcata tttggcagac agtaccctga gtgaggaaat gagtcaattt gatttctcaa
    10261 ccggagttca gagctattca tacagctccc aagaccctag acctgccact ggtcgttttc
    10321 ggagacggga gcagcgggac cccacggtgc atgatgatgt gctggagctg gagatggacg
    10381 agctcaatcg gcatgagtgc atggcgcccc tgacggccct ggtcaagcac atgcacagaa
    10441 gcctgggccc gcctcaagga gaagaggatt cagtgccaag agatcttcct tcttggatga
    10501 aattcctcca tggcaaactg ggaaatccaa tagtaccatt aaatatccgt ctcttcttag
    10561 ccaagcttgt tattaataca gaagaggtct ttcgccctta cgcgaagcac tggcttagcc
    10621 ccttgctgca gctggctgct tctgaaaaca atggaggaga aggaattcac tacatggtgg
    10681 ttgagatagt ggccactatt ctttcatgga caggcttggc cactccaaca ggggtcccta
    10741 aagatgaagt gttagcaaat cgattgctta atttcctaat gaaacatgtc tttcatccaa
    10801 aaagagctgt gtttagacac aaccttgaaa ttataaagac ccttgtcgag tgctggaagg
    10861 attgtttatc catcccttat aggttaatat ttgaaaagtt ttccggtaaa gatcctaatt
    10921 ctaaagacaa ctcagtaggg attcaattgc taggcatcgt gatggccaat gacctgcctc
    10981 cctatgaccc acagtgtggc atccagagta gcgaatactt ccaggctttg gtgaataata
    11041 tgtcctttgt aagatataaa gaagtgtatg ccgctgcagc agaagttcta ggacttatac
    11101 ttcgatatgt tatagagaga aaaaacatac tggaggagtc tctgtgtgaa ctggttgcga
    11161 aacaattgaa gcaacatcag aatactatgg aggacaagtt tattgtgtgc ttgaacaaag
    11221 tgaccaagag cttccctcct cttgcagaca ggttcatgaa tgctgtgttc tttctgctgc
    11281 caaaatttca tggagtgttg aaaacactct gtctggaggt ggtactttgt cgtgtggagg
    11341 gaatgacaga gctgtacttc cagttaaaga gcaaggactt cgttcaagtc atgagacata
    11401 gagatgatga aagacaaaaa gtatgtttgg acataattta taagatgatg ccaaagttaa
    11461 aaccagtaga actccgagaa cttctgaacc ccgttgtgga attcgtttcc catccttcta
    11521 caacatgtag ggaacaaatg tataatattc tcatgtggat tcatgataat tacagagatc
    11581 cagaaagtga gacagataat gactcccagg aaatatttaa gttggcaaaa gatgtgctga
    11641 ttcaaggatt gatcgatgag aaccctggac ttcaattaat tattcgaaat ttctggagcc
    11701 atgaaactag gttaccttca aataccttgg accggttgct ggcactaaat tccttatatt
    11761 ctcctaagat agaagtgcac tttttaagtt tagcaacaaa ttttctgctc gaaatgacca
    11821 gcatgagccc agattatcca aaccccatgt tcgagcatcc tctgtcagaa tgcgaatttc
    11881 aggaatatac cattgattct gattggcgtt tccgaagtac tgttctcact ccgatgtttg
    11941 tggagaccca ggcctcccag ggcactctcc aggcccgtac ccaggaaggg tccctctcag
    12001 ctcgctggcc agtggcaggg cagataaggg ccacccagca gcagcatgac ttcacactga
    12061 cacagactgc agatggaaga agctcatttg attggctgac cgggagcagc actgacccgc
    12121 tggtcgacca caccagtccc tcatctgact ccttgctgtt tgcccacaag aggagtgaaa
    12181 ggttacagag agcacccttg aagtcagtgg ggcctgattt tgggaaaaaa aggctgggcc
    12241 ttccagggga cgaggtggat aacaaagtga aaggtgcggc cggccggacg gacctactac
    12301 gactgcgcag acggtttatg agggaccagg agaagcttag tttgatgtat gcccgaaaag
    12361 gcgttgctga gcaaaaacga gagaaggaaa tcaagagtga gttaaaaatg aagcaggatg
    12421 cccaggtcgt tctgtacaga agctaccggc acggagacct tcctgacatt cagatcaagc
    12481 acagcagcct catcaccccg ttacaggccg tggcccagag ggacccaata atcgcgaaac
    12541 agctctttag cagcttgttt tctggaattt tgaaagagat ggataaattt aagacactgt
    12601 ctgaaaaaaa caacatcact caaaagttgc ttcaagactt caatcgtttt cttaatacca
    12661 ccttctcttt ctttccaccc tttgtctctt gtattcagga cattagctgt cagcacgcag
    12721 ccctgctgag cctcgaccca gcggctgtta gcgctggttg cctggccagc ctacagcagc
    12781 ctgttggcat ccgcctacta gaggaggcta ttctttgtct gctgcctgct gagctgcccg
    12841 ccaagcgagt ccgtgggaag gctagacctc ctcctgatgt cctcagatgg gtggagcttg
    12901 ctaagctgta tagatcaatt ggagaatacg acgtcctccg tgggattttt accagtgaga
    12961 taggaacaaa gcaaatcact cagagtgcat tattagcaga agccagaagt gattattctg
    13021 aagctgctaa gcagtatgat gaggctctca ataaacaaga ctgggtagat ggtgagccca
    13081 cagaagccga gaaggatttt tgggaacttg catcccttga ctgttacaac caccttgctg
    13141 agtggaaatc acttgaatac tgttctacag ccagtataga cagtgagaac cccccagacc
    13201 taaataaaat ctggagtgaa ccattttatc aggaaacata tctaccttac atgatccgca
    13261 gcaagctgaa gctgctgctc cagggagagg ctgaccagtc cctgctgaca tttattgaca
    13321 aagctatgca cggggagctc cagaaggcga ttctagagct tcattacagt caagagctga
    13381 gtctgcttta cctcctgcaa gatgatgttg acagagccaa atattacatt caaaatggca
    13441 ttcagagttt tatgcagaat tattctagta ttgatgtcct cttacaccaa agtagactca
    13501 ccaaattgca gtctgtacag gctttaacag aaattcagga gttcatcagc tttataagca
    13561 aacaaggcaa tttatcatct caagttcccc ttaagagact tctgaacacc tgggcaaaca
    13621 gatatccaga tgctaaaatg gacccaatga acatctggga tgacatcatc acaaatcgat
    13681 gtttctttct cagcaaaata gaggagaagc ttacccctct tccagaagat aatagtatga
    13741 atgtggatca agatggagac cccagtgaca ggatggaagt gcaagagcag gaagaagata
    13801 tcagctccct gatcaggagt tgcaagtttt ccatgaaaat gaagatgata gacagtgccc
    13861 ggaagcagaa caatttctca cttgctatga aactactgaa ggagctgcat aaagagtcaa
    13921 aaaccagaga cgattggctg gtgagctggg tgcagagcta ctgccgcctg agccactgcc
    13981 ggagccggtc ccagggctgc tctgagcagg tgctcactgt gctgaaaaca gtctctttgt
    14041 tggatgagaa caacgtgtca agctacttaa gcaaaaatat tctggctttc cgtgaccaga
    14101 acattctctt gggtacaact tacaggatca tagcgaatgc tctcagcagt gagccagcct
    14161 gccttgctga aatcgaggag gacaaggcta gaagaatctt agagctttct ggatccagtt
    14221 cagaggattc agagaaggtg atcgcgggtc tgtaccagag agcattccag cacctctctg
    14281 aggctgtgca ggcggctgag gaggaggccc agcctccctc ctggagctgt gggcctgcag
    14341 ctggggtgat tgatgcttac atgacgctgg cagatttctg tgaccaacag ctgcgcaagg
    14401 aggaagagaa tgcatcagtt actgattctg cagaactgca ggcgtatcca gcacttgtgg
    14461 tggagaaaat gttgaaagct ttaaaattaa attccaatga agccagattg aagtttccta
    14521 gattacttca gattatagaa cggtatccag aggagacttt gagcctcatg acaaaagaga
    14581 tctcttccgt tccctgctgg cagttcatca gctggatcag ccacatggtg gccttactgg
    14641 acaaagacca agccgttgct gttcagcact ctgtggaaga aatcactgat aactacccgc
    14701 aggctattgt ttatcccttc atcataagca gcgaaagcta ttccttcaag gatacttcta
    14761 ctggtcataa gaataaggag tttgtggcaa ggattaaaag taagttggat caaggaggag
    14821 tgattcaaga ttttattaat gccttagatc agctctctaa tcctgaactg ctctttaagg
    14881 attggagcaa tgatgtaaga gctgaactag caaaaacccc tgtaaataaa aaaaacattg
    14941 aaaaaatgta tgaaagaatg tatgcagcct tgggtgaccc aaaggctcca ggcctggggg
    15001 cctttagaag gaagtttatt cagacttttg gaaaagaatt tgataaacat tttgggaaag
    15061 gaggttctaa actactgaga atgaagctca gtgacttcaa cgacattacc aacatgctac
    15121 ttttaaaaat gaacaaagac tcaaagcccc ctgggaatct gaaagaatgt tcaccctgga
    15181 tgagcgactt caaagtggag ttcctgagaa atgagctgga gattcccggt cagtatgacg
    15241 gtaggggaaa gccattgcca gagtaccacg tgcgaatcgc cgggtttgat gagcgggtga
    15301 cagtcatggc gtctctgcga aggcccaagc gcatcatcat ccgtggccat gacgagaggg
    15361 aacacccttt cctggtgaag ggtggcgagg acctgcggca ggaccagcgc gtggagcagc
    15421 tcttccaggt catgaatggg atcctggccc aagactccgc ctgcagccag agggccctgc
    15481 agctgaggac ctatagcgtt gtgcccatga cctccaggtt aggattaatt gagtggcttg
    15541 aaaatactgt taccttgaag gaccttcttt tgaacaccat gtcccaagag gagaaggcgg
    15601 cttacctgag tgatcccagg gcaccgccgt gtgaatataa agattggctg acaaaaatgt
    15661 caggaaaaca tgatgttgga gcttacatgc taatgtataa gggcgctaat cgtactgaaa
    15721 cagtcacgtc ttttagaaaa cgagaaagta aagtgcctgc tgatctctta aagcgggcct
    15781 tcgtgaggat gagtacaagc cctgaggctt tcctggcgct ccgctcccac ttcgccagct
    15841 ctcacgctct gatatgcatc agccactgga tcctcgggat tggagacaga catctgaaca
    15901 actttatggt ggccatggag actggtggcg tgatcgggat cgactttggg catgcgtttg
    15961 gatccgctac acagtttctg ccagtccctg agttgatgcc ttttcggcta actcgccagt
    16021 ttatcaatct gatgttacca atgaaagaaa cgggccttat gtacagcatc atggtacacg
    16081 cactccgggc cttccgctca gaccctggcc tgctcaccaa caccatggat gtgtttgtca
    16141 aggagccctc ctttgattgg aaaaattttg aacagaaaat gctgaaaaaa ggagggtcat
    16201 ggattcaaga aataaatgtt gctgaaaaaa attggtaccc ccgacagaaa atatgttacg
    16261 ctaagagaaa gttagcaggt gccaatccag cagtcattac ttgtgatgag ctactcctgg
    16321 gtcatgagaa ggcccctgcc ttcagagact atgtggctgt ggcacgagga agcaaagatc
    16381 acaacattcg tgcccaagaa ccagagagtg ggctttcaga agagactcaa gtgaagtgcc
    16441 tgatggacca ggcaacagac cccaacatcc ttggcagaac ctgggaagga tgggagccct
    16501 ggatgtgagg tctgcggccg cgatctgtcg acgataaacc cgctgatcag cctcgactgt
    16561 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
    16621 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
    16681 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
    16741 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
    16801 cagatcggat ccatcgatat aacttcgtat aggagacttt atacgaagtt atctcgatga
    16861 aggggttctc tagatcgaga attcactggc cgtcgtttta caaggcgcgc ccccgggtac
    16921 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcg
//
