LOCUS       sequence-468245-         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of NFAST on the ER membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Garcia Casas P, Rossini M, Pavenius L, Saeed M, Arnst N, Sonda S, 
            Fernandes T, D'Arsie I, Bruzzone M, Berno V, Raimondi A, Sassano ML,
            Naia L, Barbieri E, Sigismund S, Agostinis P, Sturlese M, Niemeyer 
            BA, Brismar H, Ankarcrona M, Gautier A, Pizzo P, Filadi R
  TITLE     Simultaneous detection of membrane contact dynamics and associated 
            Ca(2+) signals by reversible chemogenetic reporters.
  JOURNAL   Nat Commun. 2024 Nov 12;15(1):9775. doi: 10.1038/s41467-024-52985-0.
  PUBMED    39532847
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-11-12"; volume: "15(1):9775. doi"; pages: " 
            10.1038/s41467-024-52985-0"
COMMENT     Sequence Label: ER-NFAST
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagcttgcca ccatggaccc tgtggtggtg ctggggctct gtctctcctg tttgcttctc
       61 ctttcactct ggaaacagag ctatggggga gggtctggtg gatctggagg taccgatatc
      121 gtctccggag gaggcggcag cggcggaggg ggatccatgg agcatgttgc ctttggcagt
      181 gaggacatcg agaacactct ggccaaaatg gacgacggac aactggatgg gttggccttt
      241 ggcgcaattc agctcgatgg tgacgggaat atcctgcagt acaatgctgc tgaaggagac
      301 atcacaggca gagatcccaa acaggtgatt gggaagaact tcttcaagga tgttgcacct
      361 ggaacggatt ctcccgagtt ttacggcaaa ttcaaggaag gcgtagcgtc agggaatctg
      421 aacaccatgt tcgaatggat gataccgaca agcaggggac caaccaaggt caaggtgcac
      481 atgaagaaag ccctttccag cggccgctaa ctcgag
//
