LOCUS       sequence-468249-         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of RspA-NFAST on the ER membrane after a 55 aminoacid 
            linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Garcia Casas P, Rossini M, Pavenius L, Saeed M, Arnst N, Sonda S, 
            Fernandes T, D'Arsie I, Bruzzone M, Berno V, Raimondi A, Sassano ML,
            Naia L, Barbieri E, Sigismund S, Agostinis P, Sturlese M, Niemeyer 
            BA, Brismar H, Ankarcrona M, Gautier A, Pizzo P, Filadi R
  TITLE     Simultaneous detection of membrane contact dynamics and associated 
            Ca(2+) signals by reversible chemogenetic reporters.
  JOURNAL   Nat Commun. 2024 Nov 12;15(1):9775. doi: 10.1038/s41467-024-52985-0.
  PUBMED    39532847
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-11-12"; volume: "15(1):9775. doi"; pages: " 
            10.1038/s41467-024-52985-0"
COMMENT     Sequence Label: ER-long-RspA-NFAST
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             115..138
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aagcttgcca ccatggaccc tgtggtggtg ctggggctct gtctctcctg tttgcttctc
       61 ctttcactct ggaaacagag ctatggggga gggtctggtg gatctggagg taccgactac
      121 aaagacgatg acgacaaggg cagcggagga ggctccggag gaggcggcag cggcggaggg
      181 aagaaaagcg gaggcggagg ctccggagga ggcggcagcg gcggagggaa gaaatccgga
      241 ggaggcggca gcggcggagg gtctggtgga tccatggaga ccgtgagatt cggcggcgac
      301 gacatcgaga acagcctggc caagatggac gacaaggccc tggacaagct ggccttcggc
      361 gccatccagc tggacggcaa cggcaagatc atccactaca acgccgccga gggcaccatc
      421 accggcagag accccaagac cgtgatcggc aagaacttct tcaccgacgt ggcccccggc
      481 acccagagca aggagttcca gggcagattc aaggagggcg tgcagaaggg cgacctgaac
      541 accatgttcg agtggatgat ccccaccagc agaggcccca ccaaggtgaa ggtgcacatg
      601 aagaaggcca tgacctaaga attc
//
