LOCUS       sequence-46825-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pMartini Gate A R2-R1
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..178
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(203..208,209..226,227..233)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(353..372)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcggccgcc ggcctcgagg gccggatccg aattcccggg agagctcgat atcacaagtt
       61 tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
      121 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggc
      181 ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat
      241 tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
      301 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
      361 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
      421 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
      481 cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg
      541 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
      601 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
      661 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
      721 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
      781 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
      841 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
      901 taatgaatta caacagt
//
