LOCUS       sequence-468251-         510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of frNFAST on the PM facing the cytosol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Garcia Casas P, Rossini M, Pavenius L, Saeed M, Arnst N, Sonda S, 
            Fernandes T, D'Arsie I, Bruzzone M, Berno V, Raimondi A, Sassano ML,
            Naia L, Barbieri E, Sigismund S, Agostinis P, Sturlese M, Niemeyer 
            BA, Brismar H, Ankarcrona M, Gautier A, Pizzo P, Filadi R
  TITLE     Simultaneous detection of membrane contact dynamics and associated 
            Ca(2+) signals by reversible chemogenetic reporters.
  JOURNAL   Nat Commun. 2024 Nov 12;15(1):9775. doi: 10.1038/s41467-024-52985-0.
  PUBMED    39532847
REFERENCE   2  (bases 1 to 510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-11-12"; volume: "15(1):9775. doi"; pages: " 
            10.1038/s41467-024-52985-0"
COMMENT     Sequence Label: PM-frNFAST
FEATURES             Location/Qualifiers
     source          1..510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..84
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aagcttgcca ccatgggctg catcaagtcc aagggcaagg actccgccgg cggcgaacaa
       61 aaacttattt ctgaagagga cttggaattc ggcagcggag gaggctccgg aggaggcggc
      121 agcggcggag ggaagaaaag cggaggcgga ggctccggaa tggagcatgt tgcctttggc
      181 agtgaggaca tcgagaacac tttggccaaa atggacgacg gacaactgga tgggttggcc
      241 tttggcgcaa ttcagctcga tggtgacggg aatatcctgc agtacaatgc tgctgaagga
      301 gacatcacag gcagagatcc caaacaggtg attgggaaga acttattcaa ggatgttgca
      361 cctggaacgg tttcctccgg gttttacggc aaattcaagg aaggcgtagc gtcagggaat
      421 ctgaacacca tgttcgaatg gatgataccg acaagcaggg gaccaaccaa ggtcaagata
      481 cacatgaaga aagccctttc ctaaggtacc
//
