LOCUS       sequence-468253-       15945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low copy number provides expression of human DNA-PKcs with a single 
            K>R substitution that ablates kinase activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15945)
  AUTHORS   Buehl CJ, Goff NJ, Hardwick SW, Gellert M, Blundell TL, Yang W, 
            Chaplin AK, Meek K
  TITLE     Two distinct long-range synaptic complexes promote different aspects
            of end processing prior to repair of DNA breaks by non-homologous 
            end joining.
  JOURNAL   Mol Cell. 2023 Mar 2;83(5):698-714.e4. doi: 
            10.1016/j.molcel.2023.01.012. Epub 2023 Jan 31.
  PUBMED    36724784
REFERENCE   2  (bases 1 to 15945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.01.012"; journalName: "Mol Cell"; date: 
            "2023-03-2- 2"; volume: "83"; issue: "5"; pages: "698-714.e4"
COMMENT     Sequence Label: RMCE K>R DNA-PKcs
FEATURES             Location/Qualifiers
     source          1..15945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..37)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        105..209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(210..278,279..1070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(428..447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1241..1829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1730..1749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1983..2000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2065..2087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2084..2100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2084..2100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2134..2167
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2311..2614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(15519..15536)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15525..15749
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(15787..15820)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(15853..15869)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15902..15918)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15911..15933)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
       61 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      421 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
      481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
      721 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
      781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
      841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
      901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
      961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1381 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1981 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     2041 atctgtgcgg tatttcacac cgcaagcgga taacaatttc acacaggaaa cagctatgac
     2101 catgattacg ccaagcttgc atgcctgcag gggataactt cgtataatgt atgctatacg
     2161 aagttatggg gccgctctag cctcgaggcc gtaatgagac gcacaaacta atatcacaaa
     2221 ctggaaatgt ctatcaatat atagttgctc tagttattaa tagtaatcaa ttacggggtc
     2281 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2341 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2401 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2461 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2521 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2581 gtacatctac gtattagtca tcgctattac catgcatggt cgaggtgagc cccacgttct
     2641 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     2701 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcccgg gggggggggg
     2761 gggggggggg gggggggggg gggggggggg ggggggggcg aggggcgggg cggggcgagg
     2821 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     2881 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     2941 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     3001 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctccttc gggctgtaat
     3061 tagcatcacg cgtggttttt ccgctagcca tggcgggctc cggagccggt gtgcgttgct
     3121 ccctgctgcg gctgcaggag accttgtccg ctgcggaccg ctgcggtgct gccctggccg
     3181 gtcatcaact gatccgcggc ctggggcagg aatgcgtcct gagcagcagc cccgcggtgc
     3241 tggcattaca gacatcttta gttttttcca gagatttcgg tttgcttgta tttgtccgga
     3301 agtcactcaa cagtattgaa tttcgtgaat gtagagaaga aatcctaaag tttttatgta
     3361 ttttcttaga aaaaatgggc cagaagatcg caccttactc tgttgaaatt aagaacactt
     3421 gtaccagtgt ttatacaaaa gatagagctg ctaaatgtaa aattccagcc ctggaccttc
     3481 ttattaagtt acttcagact tttagaagtt ctagactcat ggatgaattt aaaattggag
     3541 aattatttag taaattctat ggagaacttg cattgaaaaa aaaaatacca gatacagttt
     3601 tagaaaaagt atatgagctc ctaggattat tgggtgaagt tcatcctagt gagatgataa
     3661 ataatgcaga aaacctgttc cgcgcttttc tgggtgaact taagacccag atgacatcag
     3721 cagtaagaga gcccaaacta cctgttctgg caggatgtct gaaggggttg tcctcacttc
     3781 tgtgcaactt cactaagtcc atggaagaag atccccagac ttcaagggag atttttaatt
     3841 ttgtactaaa ggcaattcgt cctcagattg atctgaagag atatgctgtg ccctcagctg
     3901 gcttgcgcct atttgccctg catgcatctc agtttagcac ctgccttctg gacaactacg
     3961 tgtctctatt tgaagtcttg ttaaagtggt gtgcccacac aaatgtagaa ttgaaaaaag
     4021 ctgcactttc agccctggaa tcctttctga aacaggtttc taatatggtg gcgaaaaatg
     4081 cagaaatgca taaaaataaa ctgcagtact ttatggagca gttttatgga atcatcagaa
     4141 atgtggattc gaacaacaag gagttatcta ttgctatccg tggatatgga ctttttgcag
     4201 gaccgtgcaa ggttataaac gcaaaagatg ttgacttcat gtacgttgag ctcattcagc
     4261 gctgcaagca gatgttcctc acccagacag acactggtga cgaccgtgtt tatcagatgc
     4321 caagcttcct ccagtctgtt gcaagcgtct tgctgtacct tgacacagtt cctgaggtgt
     4381 atactccagt tctggagcac ctcgtggtga tgcagataga cagtttccca cagtacagtc
     4441 caaaaatgca gctggtgtgt tgcagagcca tagtgaaggt gttcctagct ttggcagcaa
     4501 aagggccagt tctcaggaat tgcattagta ctgtggtgca tcagggttta atcagaatat
     4561 gttctaaacc agtggtcctt ccaaagggac ctgagtctga atctgaagac caccgtgctt
     4621 caggggaagt cagaactggc aaatggaagg tgcccacata caaagactac gtggatctct
     4681 tcagacatct cctgagctct gaccagatga tggattctat tttagcagat gaagcatttt
     4741 tctctgtgaa ttcctccagt gaaagtctga atcatttact ttatgatgaa tttgtaaaat
     4801 ccgttttgaa gattgttgag aaattggatc ttacacttga aatacagact gttggggaac
     4861 aagagaatgg agatgaggca cctggtgttt ggatgatccc aacttcagat ccagcggcta
     4921 acttgcatcc agctaaacct aaagattttt cggctttcat taacctggtg gaattttgca
     4981 gagagattct ccctgagaaa caagcagaat tttttgaacc atgggtgtac tcattttcat
     5041 atgaattaat tttgcaatct acaaggttgc ccctcatcag tggtttctac aaattgcttt
     5101 ctattacagt aagaaatgcc aagaaaataa aatatttcga gggagttagt ccaaagagtc
     5161 tgaaacactc tcctgaagac ccagaaaagt attcttgctt tgctttattt gtgaaatttg
     5221 gcaaagaggt ggcagttaaa atgaagcagt acaaagatga acttttggcc tcttgtttga
     5281 cctttcttct gtccttgcca cacaacatca ttgaactcga tgttagagcc tacgttcctg
     5341 cactgcagat ggctttcaaa ctgggcctga gctatacccc cttggcagaa gtaggactga
     5401 atgctctaga agaatggtca atttatattg acagacatgt aatgcagcct tattacaaag
     5461 acattctccc ctgcctggat ggatacctga agacttcagc cttgtcagat gagaccaaga
     5521 ataactggga agtgtcagct ctttctcggg ctgcccagaa aggatttaat aaagtggtgt
     5581 taaagcatct gaagaagaca aagaaccttt catcaaacga agcaatatcc ttagaagaaa
     5641 taagaattag agtagtacaa atgcttggat ctctaggagg acaaataaac aaaaatcttc
     5701 tgacagtcac gtcctcagat gagatgatga agagctatgt ggcctgggac agagagaagc
     5761 ggctgagctt tgcagtgccc tttagagaga tgaaacctgt cattttcctg gatgtgttcc
     5821 tgcctcgagt cacagaatta gcgctcacag ccagtgacag acaaactaag gttgcagcct
     5881 gtgaactttt acatagcatg gttatgttta tgttgggcaa agccacgcag atgccagaag
     5941 ggggacaggg agccccaccc atgtaccagc tctataagcg gacgtttcct gtgctgcttc
     6001 gacttgcgtg tgatgttgat caggtgacaa ggcaactgta tgagccacta gttatgcagc
     6061 tgattcactg gttcactaac aacaagaaat ttgaaagtca ggatactgtt gccttactag
     6121 aagctatatt ggatggaatt gtggaccctg ttgacagtac tttaagagat ttttgtggtc
     6181 ggtgtattcg agaattcctt aaatggtcca ttaagcaaat aacaccacag cagcaggaga
     6241 agagtccagt aaacaccaaa tcgcttttca agcgacttta tagccttgcg cttcacccca
     6301 atgctttcaa gaggctggga gcatcacttg cctttaataa tatctacagg gaattcaggg
     6361 aagaagagtc tctggtggaa cagtttgtgt ttgaagcctt ggtgatatac atggagagtc
     6421 tggccttagc acatgcagat gagaagtcct taggtacaat tcaacagtgt tgtgatgcca
     6481 ttgatcacct atgccgcatc attgaaaaga agcatgtttc tttaaataaa gcaaagaaac
     6541 gacgtttgcc gcgaggattt ccaccttccg catcattgtg tttattggat ctggtcaagt
     6601 ggcttttagc tcattgtggg aggccccaga cagaatgtcg acacaaatcc attgaactct
     6661 tttataaatt cgttccttta ttgccaggca acagatcccc taatttgtgg ctgaaagatg
     6721 ttctcaagga agaaggtgtc tcttttctca tcaacacctt tgaggggggt ggctgtggcc
     6781 agccctcggg catcctggcc cagcccaccc tcttgtacct tcgggggcca ttcagcctgc
     6841 aggccacgct atgctggctg gacctgctcc tggccgcgtt ggagtgctac aacacgttca
     6901 ttggcgagag aactgtagga gcgctccagg tcctaggtac tgaagcccag tcttcacttt
     6961 tgaaagcagt ggctttcttc ttagaaagca ttgccatgca tgacattata gcagcagaaa
     7021 agtgctttgg cactggggca gcaggtaaca gaacaagccc acaagaggga gaaaggtaca
     7081 actacagcaa atgcaccgtt gtggtccgga ttatggagtt taccacgact ctgctaaaca
     7141 cctccccgga aggatggaag ctcctgaaga aggacttgtg taatacacac ctgatgagag
     7201 tcctggtgca gacgctgtgt gagcccgcaa gcataggttt caacatcgga gacgtccagg
     7261 ttatggctca tcttcctgat gtttgtgtga atctgatgaa agctctaaag atgtccccat
     7321 acaaagatat cctagagacc catctgagag agaaaataac agcacagagc attgaggagc
     7381 tttgtgccgt caacttgtat ggccctgacg cgcaagtgga caggagcagg ctggctgctg
     7441 ttgtgtctgc ctgtaaacag cttcacagag ctgggcttct gcataatata ttaccgtctc
     7501 agtccacaga tttgcatcat tctgttggca cagaacttct ttccctggtt tataaaggca
     7561 ttgcccctgg agatgagaga cagtgtctgc cttctctaga cctcagttgt aagcagctgg
     7621 ccagcggact tctggagtta gcctttgctt ttggaggact gtgtgagcgc cttgtgagtc
     7681 ttctcctgaa cccagcggtg ctgtccacgg cgtccttggg cagctcacag ggcagcgtca
     7741 tccacttctc ccatggggag tatttctata gcttgttctc agaaacgatc aacacggaat
     7801 tattgaaaaa tctggatctt gctgtattgg agctcatgca gtcttcagtg gataatacca
     7861 aaatggtgag tgccgttttg aacggcatgt tagaccagag cttcagggag cgagcaaacc
     7921 agaaacacca aggactgaaa cttgcgacta caattctgca acactggaag aagtgtgatt
     7981 catggtgggc caaagattcc cctctcgaaa ctaaaatggc agtgctggcc ttactggcaa
     8041 aaattttaca gattgattca tctgtatctt ttaatacaag tcatggttca ttccctgaag
     8101 tctttacaac atatattagt ctacttgctg acacaaagct ggatctacat ttaaagggcc
     8161 aagctgtcac tcttcttcca ttcttcacca gcctcactgg aggcagtctg gaggaactta
     8221 gacgtgttct ggagcagctc atcgttgctc acttccccat gcagtccagg gaatttcctc
     8281 caggaactcc gcggttcaat aattatgtgg actgcatgaa aaagtttcta gatgcattgg
     8341 aattatctca aagccctatg ttgttggaat tgatgacaga agttctttgt cgggaacagc
     8401 agcatgtcat ggaagaatta tttcaatcca gtttcaggag gattgccaga aggggttcat
     8461 gtgtcacaca agtaggcctt ctggaaagcg tgtatgaaat gttcaggaag gatgaccccc
     8521 gcctaagttt cacacgccag tcctttgtgg accgctccct cctcactctg ctgtggcact
     8581 gtagcctgga tgctttgaga gaattcttca gcacaattgt ggtggatgcc attgatgtgt
     8641 tgaagtccag gtttacaaag ctaaatgaat ctacctttga tactcaaatc accaagaaga
     8701 tgggctacta taagattcta gacgtgatgt attctcgcct tcccaaagat gatgttcatg
     8761 ctaaggaatc aaaaattaat caagttttcc atggctcgtg tattacagaa ggaaatgaac
     8821 ttacaaagac attgattaaa ttgtgctacg atgcatttac agagaacatg gcaggagaga
     8881 atcagctgct ggagaggaga agactttacc attgtgcagc atacaactgc gccatatctg
     8941 tcatctgctg tgtcttcaat gagttaaaat tttaccaagg ttttctgttt agtgaaaaac
     9001 cagaaaagaa cttgcttatt tttgaaaatc tgatcgacct gaagcgccgc tataattttc
     9061 ctgtagaagt tgaggttcct atggaaagaa agaaaaagta cattgaaatt aggaaagaag
     9121 ccagagaagc agcaaatggg gattcagatg gtccttccta tatgtcttcc ctgtcatatt
     9181 tggcagacag taccctgagt gaggaaatga gtcaatttga tttctcaacc ggagttcaga
     9241 gctattcata cagctcccaa gaccctagac ctgccactgg tcgttttcgg agacgggagc
     9301 agcgggaccc cacggtgcat gatgatgtgc tggagctgga gatggacgag ctcaatcggc
     9361 atgagtgcat ggcgcccctg acggccctgg tcaagcacat gcacagaagc ctgggcccgc
     9421 ctcaaggaga agaggattca gtgccaagag atcttccttc ttggatgaaa ttcctccatg
     9481 gcaaactggg aaatccaata gtaccattaa atatccgtct cttcttagcc aagcttgtta
     9541 ttaatacaga agaggtcttt cgcccttacg cgaagcactg gcttagcccc ttgctgcagc
     9601 tggctgcttc tgaaaacaat ggaggagaag gaattcacta catggtggtt gagatagtgg
     9661 ccactattct ttcatggaca ggcttggcca ctccaacagg ggtccctaaa gatgaagtgt
     9721 tagcaaatcg attgcttaat ttcctaatga aacatgtctt tcatccaaaa agagctgtgt
     9781 ttagacacaa ccttgaaatt ataaagaccc ttgtcgagtg ctggaaggat tgtttatcca
     9841 tcccttatag gttaatattt gaaaagtttt ccggtaaaga tcctaattct aaagacaact
     9901 cagtagggat tcaattgcta ggcatcgtga tggccaatga cctgcctccc tatgacccac
     9961 agtgtggcat ccagagtagc gaatacttcc aggctttggt gaataatatg tcctttgtaa
    10021 gatataaaga agtgtatgcc gctgcagcag aagttctagg acttatactt cgatatgtta
    10081 tagagagaaa aaacatactg gaggagtctc tgtgtgaact ggttgcgaaa caattgaagc
    10141 aacatcagaa tactatggag gacaagttta ttgtgtgctt gaacaaagtg accaagagct
    10201 tccctcctct tgcagacagg ttcatgaatg ctgtgttctt tctgctgcca aaatttcatg
    10261 gagtgttgaa aacactctgt ctggaggtgg tactttgtcg tgtggaggga atgacagagc
    10321 tgtacttcca gttaaagagc aaggacttcg ttcaagtcat gagacataga gatgatgaaa
    10381 gacaaaaagt atgtttggac ataatttata agatgatgcc aaagttaaaa ccagtagaac
    10441 tccgagaact tctgaacccc gttgtggaat tcgtttccca tccttctaca acatgtaggg
    10501 aacaaatgta taatattctc atgtggattc atgataatta cagagatcca gaaagtgaga
    10561 cagataatga ctcccaggaa atatttaagt tggcaaaaga tgtgctgatt caaggattga
    10621 tcgatgagaa ccctggactt caattaatta ttcgaaattt ctggagccat gaaactaggt
    10681 taccttcaaa taccttggac cggttgctgg cactaaattc cttatattct cctaagatag
    10741 aagtgcactt tttaagttta gcaacaaatt ttctgctcga aatgaccagc atgagcccag
    10801 attatccaaa ccccatgttc gagcatcctc tgtcagaatg cgaatttcag gaatatacca
    10861 ttgattctga ttggcgtttc cgaagtactg ttctcactcc gatgtttgtg gagacccagg
    10921 cctcccaggg cactctccag gcccgtaccc aggaagggtc cctctcagct cgctggccag
    10981 tggcagggca gataagggcc acccagcagc agcatgactt cacactgaca cagactgcag
    11041 atggaagaag ctcatttgat tggctgaccg ggagcagcac tgacccgctg gtcgaccaca
    11101 ccagtccctc atctgactcc ttgctgtttg cccacaagag gagtgaaagg ttacagagag
    11161 cacccttgaa gtcagtgggg cctgattttg ggaaaaaaag gctgggcctt ccaggggacg
    11221 aggtggataa caaagtgaaa ggtgcggccg gccggacgga cctactacga ctgcgcagac
    11281 ggtttatgag ggaccaggag aagctcagtt tgatgtatgc cagaaaaggc gttgctgagc
    11341 aaaaacgaga gaaggaaatc aagagtgagt taaaaatgaa gcaggatgcc caggtcgttc
    11401 tgtacagaag ctaccggcac ggagaccttc ctgacattca gatcaagcac agcagcctca
    11461 tcaccccgtt acaggccgtg gcccagaggg acccaataat tgcaaaacag ctctttagca
    11521 gcttgttttc tggaattttg aaagagatgg ataaatttaa gacactgtct gaaaaaaaca
    11581 acatcactca aaagttgctt caagacttca atcgttttct taataccacc ttctctttct
    11641 ttccaccctt tgtctcttgt attcaggaca ttagctgtca gcacgcagcc ctgctgagcc
    11701 tcgacccagc ggctgttagc gctggttgcc tggccagcct acagcagccc gtgggcatcc
    11761 gcctgctaga ggaggctctg ctctgcctgc tgcctgctga gctgcccgcc aagcgagtcc
    11821 gtgggaaggc ccgcccccct cctgatgtcc tcagatgggt ggagcttgct aagctgtata
    11881 gatcaattgg agaatacgac gtcctccgtg ggatttttac cagtgagata ggaacaaagc
    11941 aaatcactca gagtgcatta ttagcagaag ccagaagtga ttattctgaa gctgctaagc
    12001 agtatgatga ggctctcaat aaacaagact gggtagatgg tgagcccaca gaagccgaga
    12061 aggatttttg ggaacttgca tcccttgact gttacaacca ccttgctgag tggaaatcac
    12121 ttgaatactg ttctacagcc agtatagaca gtgagaaccc cccagaccta aataaaatct
    12181 ggagtgaacc attttatcag gaaacatatc taccttacat gatccgcagc aagctgaagc
    12241 tgctgctcca gggagaggct gaccagtccc tgctgacatt tattgacaaa gctatgcacg
    12301 gggagctcca gaaggcgatt ctagagcttc attacagtca agagctgagt ctgctttacc
    12361 tcctgcaaga tgatgttgac agagccaaat attacattca aaatggcatt cagagtttta
    12421 tgcagaatta ttctagtatt gatgtcctct tacaccaaag tagactcacc aaattgcagt
    12481 ctgtacaggc tttaacagaa attcaggagt tcatcagctt tataagcaaa caaggcaatt
    12541 tatcatctca agttcccctt aagagacttc tgaacacctg ggcaaacaga tatccagatg
    12601 ctaaaatgga cccaatgaac atctgggatg acatcatcac aaatcgatgt ttctttctca
    12661 gcaaaataga ggagaagctt acccctcttc cagaagataa tagtatgaat gtggatcaag
    12721 atggagaccc cagtgacagg atggaagtgc aagagcagga agaagatatc agctccctga
    12781 tcaggagttg caagttttcc atgaaaatga agatgataga cagtgcccgg aagcagaaca
    12841 atttctcact tgctatgaaa ctactgaagg agctgcataa agagtcaaaa accagagacg
    12901 attggctggt gagctgggtg cagagctact gccgcctgag ccactgccgg agccggtccc
    12961 agggctgctc tgagcaggtg ctcactgtgc tgaaaacagt ctctttgttg gatgagaaca
    13021 acgtgtcaag ctacttaagc aaaaatattc tggctttccg tgaccagaac attctcttgg
    13081 gtacaactta caggatcata gcgaatgctc tcagcagtga gccagcctgc cttgctgaaa
    13141 tcgaggagga caaggctaga agaatcttag agctttctgg atccagttca gaggattcag
    13201 agaaggtgat cgcgggtctg taccagagag cattccagca cctctctgag gctgtgcagg
    13261 cggctgagga ggaggcccag cctccctcct ggagctgtgg gcctgcagct ggggtgattg
    13321 atgcttacat gacgctggca gatttctgtg accaacagct gcgcaaggag gaagagaatg
    13381 catcagttac tgattctgca gaactgcagg cgtatccagc acttgtggtg gagaaaatgt
    13441 tgaaagcttt aaaattaaat tccaatgaag ccagattgaa gtttcctaga ttacttcaga
    13501 ttatagaacg gtatccagag gagactttga gcctcatgac aaaagagatc tcttccgttc
    13561 cctgctggca gttcatcagc tggatcagcc acatggtggc cttactggac aaagaccaag
    13621 ccgttgctgt tcagcactct gtggaagaaa tcactgataa ctacccgcag gctattgttt
    13681 atcccttcat cataagcagc gaaagctatt ccttcaagga tacttctact ggtcataaga
    13741 ataaggagtt tgtggcaagg attaaaagta agttggatca aggaggagtg attcaagatt
    13801 ttattaatgc cttagatcag ctctctaatc ctgaactgct ctttaaggat tggagcaatg
    13861 atgtaagagc tgaactagca aaaacccctg taaataaaaa aaacattgaa aaaatgtatg
    13921 aaagaatgta tgcagccttg ggtgacccaa aggctccagg cctgggggcc tttagaagga
    13981 agtttattca gacttttgga aaagaatttg ataaacattt tgggaaagga ggttctaaac
    14041 tactgagaat gaagctcagt gacttcaacg acattaccaa catgctactt ttaaaaatga
    14101 acaaagactc aaagccccct gggaatctga aagaatgttc accctggatg agcgacttca
    14161 aagtggagtt cctgagaaat gagctggaga ttcccggtca gtatgacggt aggggaaagc
    14221 cattgccaga gtaccacgtg cgaatcgccg ggtttgatga gcgggtgaca gtcatggcgt
    14281 ctctgcgaag gcccaagcgc atcatcatcc gtggccatga cgagagggaa caccctttcc
    14341 tggtcagggg aggcgaggac ctgcggcagg accagcgcgt ggagcagctc ttccaggtca
    14401 tgaatgggat cctggcccaa gactccgcct gcagccagag ggccctgcag ctgaggacct
    14461 atagcgttgt gcccatgacc tccaggttag gattaattga gtggcttgaa aatactgtta
    14521 ccttgaagga ccttcttttg aacaccatgt cccaagagga gaaggcggct tacctgagtg
    14581 atcccagggc accgccgtgt gaatataaag attggctgac aaaaatgtca ggaaaacatg
    14641 atgttggagc ttacatgcta atgtataagg gcgctaatcg tactgaaaca gtcacgtctt
    14701 ttagaaaacg agaaagtaaa gtgcctgctg atctcttaaa gcgggccttc gtgaggatga
    14761 gtacaagccc tgaggctttc ctggcgctcc gctcccactt cgccagctct cacgctctga
    14821 tatgcatcag ccactggatc ctcgggattg gagacagaca tctgaacaac tttatggtgg
    14881 ccatggagac tggtggcgtg atcgggatcg actttgggca tgcgtttgga tccgctacac
    14941 agtttctgcc agtccctgag ttgatgcctt ttcggctaac tcgccagttt atcaatctga
    15001 tgttaccaat gaaagaaacg ggccttatgt acagcatcat ggtacacgca ctccgggcct
    15061 tccgctcaga ccctggcctg ctcaccaaca ccatggatgt gtttgtcaag gagccctcct
    15121 ttgattggaa aaattttgaa cagaaaatgc tgaaaaaagg agggtcatgg attcaagaaa
    15181 taaatgttgc tgaaaaaaat tggtaccccc gacagaaaat atgttacgct aagagaaagt
    15241 tagcaggtgc caatccagca gtcattactt gtgatgagct actcctgggt catgagaagg
    15301 cccctgcctt cagagactat gtggctgtgg cacgaggaag caaagatcac aacattcgtg
    15361 cccaagaacc agagagtggg ctttcagaag agactcaagt gaagtgcctg atggaccagg
    15421 caacagaccc caacatcctt ggcagaacct gggaaggatg ggagccctgg atgtgaggtc
    15481 tgcggccgcg atctgtcgac gataaacccg ctgatcagcc tcgactgtgc cttctagttg
    15541 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
    15601 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
    15661 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
    15721 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gatcggatcc
    15781 atcgatataa cttcgtatag gagactttat acgaagttat ctcgatgaag gggttctcta
    15841 gatcgagaat tcactggccg tcgttttaca aggcgcgccc ccgggtaccg agctcgaatt
    15901 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcg
//
