LOCUS       sequence-46826-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pMartini Gate B R1-R2
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..179
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(220..525)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(322..341)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agcggccgcc ggcctcgagg gccggatccg aattcccggg agagctcgat atcaaccact
       61 ttgtacaaga aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
      121 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
      181 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag
      241 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat
      301 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat
      361 atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag
      421 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag
      481 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt
      541 tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct
      601 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc
      661 ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc
      721 aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac
      781 atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg
      841 gcttttagta agccggatcc agatctttac gccccgccct g
//
