LOCUS       sequence-468272-        2668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  control for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2668)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_amilCP_control_insert
FEATURES             Location/Qualifiers
     source          1..2668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..77
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             86..97
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    914..934
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      935..992
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1029..1045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1029..1045)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1084..1115
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1116..1218
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1219..1878
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1285..1304)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1902..1929
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1941..2528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2021..2040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2550..2579)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 cgtctcacca ttgctaaccg tatcgcattg gccataccta catttacggc tagctcagtc
       61 ctaggtacaa tgctagctac tagagaaaga ggagaaatac tagatgagcg tgattgcaaa
      121 gcagatgacc tataaagttt atatgagcgg caccgtgaac ggccattatt ttgaagttga
      181 aggtgatggt aaaggcaaac cgtatgaagg tgaacagacc gttaaactga ccgttaccaa
      241 aggtggtcag agacgcgata atccgcgtct caggtccgct gccgtttgca tgggatattc
      301 tgagtccgca gtgtcagtat ggtagcattc cgtttaccaa atatccggaa gatatcccgg
      361 attatgtgaa acagagcttt ccggaaggtt atacctggga acgtattatg aattttgaag
      421 atgagagacg cggctgtagc tcgtctcaga tggtgccgtt tgtaccgtta gcaatgatag
      481 cagcattcag ggtaattgct ttatctacca cgtgaaattt agcggtctga attttccgcc
      541 taatggtccg gttatgcaga aaaaaaccca aggttgggaa ccgaataccg aacgtctgta
      601 gagacggtca gacgaagcgt ctcactgttt gcacgtgatg gtatgctgct gggtaataac
      661 tttatggcac tgaaactgga aggtggtggt cattatctgt gagagacgcg ccatgtatgc
      721 gtctcatgtg tgagtttaaa accacctaca aagccaaaaa accggtgaaa atgcctggct
      781 atcattatgt tgatcgtaaa ctggatagag acgcttgcaa gatacgtctc aggatgtgac
      841 caaccacaac aaagattaca ccagcgttga acagtgcgaa attagcattg cacgtaaacc
      901 ggttgttgcc taatactagt agcggccgct gcagtccggc aaaaaagggc aaggtgtcac
      961 caccctgccc tttttcttta aaaccgaaaa gacagggaag atggccgcga agaaaactaa
     1021 cagagacggt catagctgtt tcctgactga ccatttaaat catacctgac ctccatagca
     1081 gaaagtcaaa agcctccgac cggaggcttt tgacttgatc ggcacgtaag aggttccaac
     1141 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
     1201 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     1261 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     1321 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     1381 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt
     1441 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     1501 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     1561 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     1621 cctaaagggt ttattgagaa tatgtttttt gtctcagcca atccctgggt gagtttcacc
     1681 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     1741 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     1801 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     1861 tggcagggcg gggcgtaatg agggcccaaa tgtaatcacc tggctcacct tcgggtgggc
     1921 ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1981 cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2041 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2101 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2161 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2221 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2281 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2341 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2401 gctctgctga agccagttac ctcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2521 gatctcaaga agatcctttg attttctacc gaagaaaggc ccacccgtga aggtgagcca
     2581 gtgagttgat tgcagtccag ttacgctgga gtctgaggct cgtcctgaat gatatcaagc
     2641 ttgaattcgt ttgtaaaacg acggccag
//
