LOCUS       sequence-46828-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pMartini Gate C R2-R1
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..180
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(205..210,211..228,229..235)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(355..374)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcggccgcc ggcctcgagg gccggatccg aattcccggg agagctcgat atcaaacaag
       61 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      121 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
      181 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
      241 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      301 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      361 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      421 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
      481 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
      541 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
      601 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
      661 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
      721 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
      781 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
      841 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
      901 cttaatgaat tacaacagta
//
