LOCUS       sequence-468280-        2044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRAV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2044)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRAV12-3
FEATURES             Location/Qualifiers
     source          1..2044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(374..390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(374..390)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      429..460
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        461..563
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             564..1223
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(630..649)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1247..1274
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1286..1873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1366..1385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1895..1924)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1997..2014
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1998..2014
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagat gatgaaatcc ttgagagttt tactggtgat cctgtggctt cagttaagct
       61 gggtttggag ccaacagaag gaggtggagc aggatcctgg accactcagt gttccagagg
      121 gagccattgt ttctctcaac tgcacttaca gcaacagtgc ttttcaatac ttcatgtggt
      181 acagacagta ttccagaaaa ggccctgagt tgctgatgta cacatactcc agtggtaaca
      241 aagaagatgg aaggtttaca gcacaggtcg ataaatccag caagtatatc tccttgttca
      301 tcagagactc acagcccagt gattcagcca cctacctctg ttgagacgca gggaagatgg
      361 ccgcgaagaa aacgtcatag ctgtttcctg actgaccatt taaatcatac ctgacctcca
      421 tagcagaaag tcaaaagcct ccgaccggag gcttttgact tgatcggcac gtaagaggtt
      481 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      541 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      601 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      661 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      721 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      781 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      841 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      901 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      961 atttccctaa agggtttatt gagaatatgt tttttgtctc agccaatccc tgggtgagtt
     1021 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1081 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1141 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1201 atgagtggca gggcggggcg taatgagggc ccaaatgtaa tcacctggct caccttcggg
     1261 tgggcctttc tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1321 aaaatcgatg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1381 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1441 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1501 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1561 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1621 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1681 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1741 tctgcgctct gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa
     1801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1861 aaaaggatct caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg
     1921 agccagtgag ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat
     1981 caagcttgaa ttcgtttgta aaacgacggc cagtgctaac cgtatcgcat tggccatacc
     2041 taca
//
