LOCUS       sequence-468283-        2047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRAV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2047)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRAV14/DV4
FEATURES             Location/Qualifiers
     source          1..2047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(377..393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(377..393)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      432..463
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        464..566
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             567..1226
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(633..652)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1250..1277
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1289..1876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1369..1388)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1898..1927)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     2000..2017
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2001..2017
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagat gtcactttct agcctgctga aggtggtcac agcttcactg tggctaggac
       61 ctggcattgc ccagaagata actcaaaccc aaccaggaat gttcgtgcag gaaaaggagg
      121 ctgtgactct ggactgcaca tatgacacca gtgatccaag ttatggtcta ttctggtaca
      181 agcagcccag cagtggggaa atgatttttc ttatttatca ggggtcttat gaccagcaaa
      241 atgcaacaga aggtcgctac tcattgaatt tccagaaggc aagaaaatcc gccaaccttg
      301 tcatctccgc ttcacaactg ggggactcag caatgtactt ctgttgagac gcagggaaga
      361 tggccgcgaa gaaaacgtca tagctgtttc ctgactgacc atttaaatca tacctgacct
      421 ccatagcaga aagtcaaaag cctccgaccg gaggcttttg acttgatcgg cacgtaagag
      481 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
      541 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
      601 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
      661 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
      721 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
      781 aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
      841 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
      901 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
      961 cctatttccc taaagggttt attgagaata tgttttttgt ctcagccaat ccctgggtga
     1021 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     1081 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     1141 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     1201 gcgatgagtg gcagggcggg gcgtaatgag ggcccaaatg taatcacctg gctcaccttc
     1261 gggtgggcct ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1321 acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1381 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1441 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1501 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1561 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1621 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1681 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1741 gtatctgcgc tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg
     1801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1861 aaaaaaagga tctcaagaag atcctttgat tttctaccga agaaaggccc acccgtgaag
     1921 gtgagccagt gagttgattg cagtccagtt acgctggagt ctgaggctcg tcctgaatga
     1981 tatcaagctt gaattcgttt gtaaaacgac ggccagtgct aaccgtatcg cattggccat
     2041 acctaca
//
