LOCUS       sequence-468284-        2029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRAV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2029)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRAV16
FEATURES             Location/Qualifiers
     source          1..2029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(359..375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(359..375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      414..445
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        446..548
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             549..1208
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(615..634)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1232..1259
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1271..1858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1351..1370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1880..1909)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1982..1999
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1983..1999
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagat gaagcccacc ctcatctcag tgcttgtgat aatatttata ctcagaggaa
       61 caagagccca gagagtgact cagcccgaga agctcctctc tgtctttaaa ggggccccag
      121 tggagctgaa gtgcaactat tcctattctg ggagtcctga actcttctgg tatgtccagt
      181 actccagaca acgcctccag ttactcttga gacacatctc tagagagagc atcaaaggct
      241 tcactgctga ccttaacaaa ggcgagacat ctttccacct gaagaaacca tttgctcaag
      301 aggaagactc agccatgtat tactgttgag acgcagggaa gatggccgcg aagaaaacgt
      361 catagctgtt tcctgactga ccatttaaat catacctgac ctccatagca gaaagtcaaa
      421 agcctccgac cggaggcttt tgacttgatc ggcacgtaag aggttccaac tttcaccata
      481 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
      541 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      601 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      661 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      721 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
      781 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
      841 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
      901 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
      961 ttattgagaa tatgtttttt gtctcagcca atccctgggt gagtttcacc agttttgatt
     1021 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     1081 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     1141 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     1201 gggcgtaatg agggcccaaa tgtaatcacc tggctcacct tcgggtgggc ctttctgcgt
     1261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgatgctcaa
     1321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1681 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1741 agccagttac ctcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1801 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1861 agatcctttg attttctacc gaagaaaggc ccacccgtga aggtgagcca gtgagttgat
     1921 tgcagtccag ttacgctgga gtctgaggct cgtcctgaat gatatcaagc ttgaattcgt
     1981 ttgtaaaacg acggccagtg ctaaccgtat cgcattggcc atacctaca
//
