LOCUS       sequence-468287-        2038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRAV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2038)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRAV20
FEATURES             Location/Qualifiers
     source          1..2038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(368..384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(368..384)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      423..454
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        455..557
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             558..1217
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(624..643)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1241..1268
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1280..1867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1360..1379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1889..1918)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1991..2008
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1992..2008
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagat ggagaaaatg ttggagtgtg cattcatagt cttgtggctt cagcttggct
       61 ggttgagtgg agaagaccag gtgacgcaga gtcccgaggc cctgagactc caggagggag
      121 agagtagcag tctcaactgc agttacacag tcagcggttt aagagggctg ttctggtata
      181 ggcaagatcc tgggaaaggc cctgaattcc tcttcaccct gtattcagct ggggaagaaa
      241 aggagaaaga aaggctaaaa gccacattaa caaagaagga aagctttctg cacatcacag
      301 cccctaaacc tgaagactca gccacttatc tctgttgaga cgcagggaag atggccgcga
      361 agaaaacgtc atagctgttt cctgactgac catttaaatc atacctgacc tccatagcag
      421 aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact
      481 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
      541 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
      601 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
      661 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
      721 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta
      781 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
      841 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
      901 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
      961 ctaaagggtt tattgagaat atgttttttg tctcagccaa tccctgggtg agtttcacca
     1021 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     1081 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     1141 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     1201 ggcagggcgg ggcgtaatga gggcccaaat gtaatcacct ggctcacctt cgggtgggcc
     1261 tttctgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1321 gatgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1681 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1741 ctctgctgaa gccagttacc tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1861 atctcaagaa gatcctttga ttttctaccg aagaaaggcc cacccgtgaa ggtgagccag
     1921 tgagttgatt gcagtccagt tacgctggag tctgaggctc gtcctgaatg atatcaagct
     1981 tgaattcgtt tgtaaaacga cggccagtgc taaccgtatc gcattggcca tacctaca
//
