LOCUS       sequence-46829-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pMartini Gate A R1-R2
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..183
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(208..213,214..231,232..238)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(358..377)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cggatctgca ggtcgaccag ctggccaagc ttcttctaga ggtaccgcat gcgatatcac
       61 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
      121 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
      181 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
      241 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
      301 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      361 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
      421 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
      481 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
      541 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
      601 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
      661 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
      721 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
      781 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
      841 ctgatgccgc tggcgat
//
