LOCUS       sequence-468302-        2017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRAV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2017)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRAV40
FEATURES             Location/Qualifiers
     source          1..2017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(347..363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(347..363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      402..433
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        434..536
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             537..1196
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(603..622)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1220..1247
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1259..1846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1339..1358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1868..1897)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1970..1987
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1971..1987
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagat gaactcctct ctggactttc taattctgat cttaatgttt ggaggaacca
       61 gcagcaattc agtcaagcag acgggccaaa taactgtctc ggagggagca tctgtgacta
      121 tgaactgcac atacacatcc acggggtacc ctaccctttt ctggtatgtg gaatacccca
      181 gcaaacctct gcagcttctt cagagagaga caatggaaaa cagcaaaaac ttcggaggcg
      241 gaaatattaa agacaaaaac tcccccattg tgaaatattc agtccaggta tcagactcag
      301 ccgtgtacta ctgttgagac gcagggaaga tggccgcgaa gaaaacgtca tagctgtttc
      361 ctgactgacc atttaaatca tacctgacct ccatagcaga aagtcaaaag cctccgaccg
      421 gaggcttttg acttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
      481 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
      541 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
      601 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
      661 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
      721 ttcttgcccg cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc
      781 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
      841 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
      901 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
      961 tgttttttgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     1021 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     1081 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     1141 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatgag
     1201 ggcccaaatg taatcacctg gctcaccttc gggtgggcct ttctgcgttg ctggcgtttt
     1261 tccataggct ccgcccccct gacgagcatc acaaaaatcg atgctcaagt cagaggtggc
     1321 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1381 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1441 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1501 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1561 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1621 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1681 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1741 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1801 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1861 tttctaccga agaaaggccc acccgtgaag gtgagccagt gagttgattg cagtccagtt
     1921 acgctggagt ctgaggctcg tcctgaatga tatcaagctt gaattcgttt gtaaaacgac
     1981 ggccagtgct aaccgtatcg cattggccat acctaca
//
