LOCUS       sequence-468308-        2041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRAV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2041)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRAV8-4
FEATURES             Location/Qualifiers
     source          1..2041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(371..387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(371..387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      426..457
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        458..560
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             561..1220
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(627..646)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1244..1271
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1283..1870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1363..1382)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1892..1921)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1994..2011
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1995..2011
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagat gctcctgctg ctcgtcccag tgctcgaggt gatttttacc ctgggaggaa
       61 ccagagccca gtcggtgacc cagcttggca gccatgtctc tgtctctgaa ggagccctgg
      121 ttctgttgag gtgcaactac tcatcgtctg ttccaccata tctcttctgg tatgtgcaat
      181 accccaacca aggactccag cttctcctga agtacacatc agcggccacc ctggttaaag
      241 gcatcaacgg ttttgaggct gaatttaaga agagtgaaac ctccttccac ctgacgaaac
      301 catcagccca tatgagcgac gcggctgagt acttctgttg agacgcaggg aagatggccg
      361 cgaagaaaac gtcatagctg tttcctgact gaccatttaa atcatacctg acctccatag
      421 cagaaagtca aaagcctccg accggaggct tttgacttga tcggcacgta agaggttcca
      481 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
      541 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
      601 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      661 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      721 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc
      781 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      841 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      901 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      961 tccctaaagg gtttattgag aatatgtttt ttgtctcagc caatccctgg gtgagtttca
     1021 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     1081 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     1141 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     1201 agtggcaggg cggggcgtaa tgagggccca aatgtaatca cctggctcac cttcgggtgg
     1261 gcctttctgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1321 atcgatgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1381 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1441 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1501 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1561 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1621 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1681 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1741 gcgctctgct gaagccagtt acctcggaaa aagagttggt agctcttgat ccggcaaaca
     1801 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1861 aggatctcaa gaagatcctt tgattttcta ccgaagaaag gcccacccgt gaaggtgagc
     1921 cagtgagttg attgcagtcc agttacgctg gagtctgagg ctcgtcctga atgatatcaa
     1981 gcttgaattc gtttgtaaaa cgacggccag tgctaaccgt atcgcattgg ccatacctac
     2041 a
//
