LOCUS       sequence-468316-        2046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRBV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2046)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRBV12-3
FEATURES             Location/Qualifiers
     source          1..2046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(376..392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(376..392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      431..462
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        463..565
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             566..1225
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(632..651)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1249..1276
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1288..1875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1368..1387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1897..1926)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1999..2016
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2000..2016
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcacca tggactcctg gaccttctgc tgtgtgtccc tttgcatcct ggtagcgaag
       61 catacagatg ctggagttat ccagtcaccc cgccatgagg tgacagagat gggacaagaa
      121 gtgactctga gatgtaaacc aatttcaggc cacaactccc ttttctggta cagacagacc
      181 atgatgcggg gactggagtt gctcatttac tttaacaaca acgttccgat agatgattca
      241 gggatgcccg aggatcgatt ctcagctaag atgcctaatg catcattctc cactctgaag
      301 atccagccct cagaacccag ggactcagct gtgtacttct gtgtgagacg cagggaagat
      361 ggccgcgaag aaaacgtcat agctgtttcc tgactgacca tttaaatcat acctgacctc
      421 catagcagaa agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg
      481 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
      541 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
      601 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
      661 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
      721 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
      781 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
      841 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
      901 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
      961 ctatttccct aaagggttta ttgagaatat gttttttgtc tcagccaatc cctgggtgag
     1021 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     1081 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     1141 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     1201 cgatgagtgg cagggcgggg cgtaatgagg gcccaaatgt aatcacctgg ctcaccttcg
     1261 ggtgggcctt tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1321 caaaaatcga tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1741 tatctgcgct ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc
     1801 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1861 aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca cccgtgaagg
     1921 tgagccagtg agttgattgc agtccagtta cgctggagtc tgaggctcgt cctgaatgat
     1981 atcaagcttg aattcgtttg taaaacgacg gccagtgcta accgtatcgc attggccata
     2041 cctaca
//
