LOCUS       sequence-468327-        2035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRBV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2035)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRBV29-1
FEATURES             Location/Qualifiers
     source          1..2035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(365..381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(365..381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      420..451
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        452..554
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             555..1214
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(621..640)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1238..1265
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1277..1864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1357..1376)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1886..1915)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1988..2005
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1989..2005
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcacca tgctgagtct tctgctcctt ctcctgggac taggctctgt gttcagtgct
       61 gtcatctctc aaaagccaag cagggatatc tgtcaacgtg gaacctccct gacgatccag
      121 tgtcaagtcg atagccaagt caccatgatg ttctggtacc gtcagcaacc tggacagagc
      181 ctgacactga tcgcaactgc aaatcagggc tctgaggcca catatgagag tggatttgtc
      241 attgacaagt ttcccatcag ccgcccaaac ctaacattct caactctgac tgtgagcaac
      301 atgagccctg aagacagcag catatatctc tgtgagacgc agggaagatg gccgcgaaga
      361 aaacgtcata gctgtttcct gactgaccat ttaaatcata cctgacctcc atagcagaaa
      421 gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca cgtaagaggt tccaactttc
      481 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
      541 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      601 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      661 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      721 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
      781 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
      841 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
      901 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
      961 aagggtttat tgagaatatg ttttttgtct cagccaatcc ctgggtgagt ttcaccagtt
     1021 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     1081 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     1141 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     1201 agggcggggc gtaatgaggg cccaaatgta atcacctggc tcaccttcgg gtgggccttt
     1261 ctgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgat
     1321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1681 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1741 tgctgaagcc agttacctcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1801 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1861 tcaagaagat cctttgattt tctaccgaag aaaggcccac ccgtgaaggt gagccagtga
     1921 gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata tcaagcttga
     1981 attcgtttgt aaaacgacgg ccagtgctaa ccgtatcgca ttggccatac ctaca
//
